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Shoot tips from in vitro plants of four rose species were cryopreserved by the droplet vitrification method. Optimized conditions involved exposure to loading solution for 20 min, then treatment with plant vitrification solution (PVS2) for 20 min (Rosa agrestis, R. canina and R. dumalis) or 30 min (R. rubiginosa) followed by freezing in liquid nitrogen. Survival rate ranged from 78.3 to 95.1%, depending on the species. Regrowth rate of shoot tips was 50.5% for R. agrestis, 63.2% for R. rubiginosa, 71.4% for R. dumalis and 78% for R. canina. The preculture of donor plants in a medium with 0.25 цЫ sucrose facilitated the isolation of shoot tips and increased regrowth rate after cryopreservation. Plant regeneration was carried out in Murashige and Skoog medium with 1 цЫ 6-benzylaminopurine, 1.5 цЫ gibberellic acid and 0.087 M sucrose. Plants regenerated from cryopreserved shoot tips did not display morphological alterations in comparison with non-cryopreserved shoot tip - derived plants.
Determining suitable sites for species is of great importance in the choice of plant species to be used for ecosystem restoration. The number of plant species to be used in restoration of degraded ecosystems is restricted by climate in arid and semi arid regions. Planting with economically important species in addition to ecological aspects is preferred by decision makers in Mediterrenian countries including Turkey. This study was addressed to determine the significant environmental factors influencing the distribution of dog rose hip (Rosa canina) and to obtain its distribution model. The study was carried out in an area of 26,400 km2 in The Inner Anatolia which has semiarid climate. 106 sample plots, 20 x 20 m in size, were selected from different sites with and without Rosa canina in the summer of 2007. Wilcoxon rank-sum statistic for continuous variables and Pearson Chi-square tests for categorical variables were applied. Altitude, slope degree, available water capacity, soil reaction and soil K, Ca, Mg content as well and total calcium carbonate amount as the continuous variables and northwest- northeast group of aspect, metamorphic group of bedrock as the categorical variables were determined as the significant factors influencing the presence of dog rose. Generalized Additive Model (GAM) was performed for modeling the distribution of the species. To obtain the best model, all significant environmental factors were evaluated. The best model result was obtained (training Area Under Curve (AUC) = 0.902 and cross-validation AUC = 0.841) by means of altitude, slope degree, aspect, and bedrock. Interspecific correlation analysis (ICA) was applied to define indicator species accompanying the dog rose. Dog rose distribution was positively correlated with Cistus laurifolius, Pyrus elaeagnifolia, Lonicera caucasica subsp. orientalis, Quercus vulcanica and Amelanchier rotundifolia subsp. Rotundifolia. The results obtained from this study are crucial for estimation of the suitable areas for Dog rose in restoration studies of natural ecosystems in the Mediterranean region, including Anatolia in Turkey.
This study examined the potential of liquid wild dog rose (Rosa canina) seeds oil and solid seeds residue obtained after CO2 extraction in supercritical conditions to mitigate rumen methane production in vitro. Two experiments were carried out. The substrate comprised of a mixture of meadow hay and barley meal (60:40) for the control diets (CON1 in experiment with oil and CON2 in experiment with residue). The control diets were supplemented up to 5% in dry matter of rose seeds oil (RO) and 5% of rose seeds residue (RR). The following parameters were measured: pH, ammonia, volatile fatty acids, ciliate protozoa and bacteria count, methane concentration and methanogens population. In the RO treatment a decrease in methane production and an increase in the Archea population were observed. In the RR treatment no change in methane production was reported, whereas some variations in protozoal populations were detected in relation to CON2. The potential to mitigate methane production was reported only in wild dog rose seeds oil treatment. Besides, no negative effect of wild dog rose seeds residue on rumen processes was stated, what may predispose this protein and fibre containing by-product to be utilized as ruminants dietary ingredient.
The qualitative and quantitative analysis of polycyclic aromatic hydrocarbons contamination in selected popular brands of fruit and herbal teas was performed using GC-MS method. Among all teas similar quality profiles of PAHs were observed. In all determined compounds 4 light PAHs (phenanthrene, anthracene, fluoranthene, pyrene) from the group of PAHs listed by EPA were predominant. From group of 15 PAHs, marked out for analysis by The UE Scientific Committee on Food, only benzo[a]anthracene, chrysene, 5-metylchrysene, benzo[b]fluoranthene and benzo[k]fluoranthene were stated, although, their levels were relatively low. Benzo[a]pyrene and the most carcinogenic PAHs, namely dibenzopyrenes, were not detected. Moreover, the GC-MS analysis revealed significant differences in contamination level of particular brands of teas among all fruit and herbal teas under investigation.
The studied material were the fruits of 7 wild species: dog rose (Rosa canina L.), blackberry (Rubus caesius L.), elderberry (Sambucus nigra L.), blueberry (Vaccinium myrtillus L.), blackthorn (Prunus spinosa L.), rowan (Sorbus aucuparia L.) and wild strawberry (Fragaria vesca L.). The fruits were subjected to an antioxidant activity assay (AA). Two methods were used: FRAP and ABTS. The fruit samples were also subjected to analyses for phenolics (recalculated onto gallic acid) by means of the Folin-Ciocalteau method. The fruit samples were subjected to vitamin C analyses as well, applying the Tilmans' method. Dog rose fruits were characterized by the largest antioxidant capacity - 127.78±1.85 mM Fe100 g-1 of fresh mass (FRAP method) and 38.75±0.33 ^M TEg-1 of fresh mass (ABTS method). At the same time, they con­tained the largest amounts of vitamin C - 1252.37±6.58 mg100 g-1 of fresh mass and phenolics - 3217.28±11.94 mg100 g-1 of fresh mass. Blackthorn, rowan and wild strawberry had the lowest antioxidant capacity of all the fruits studied. The examin­ation confirmed a high correlation between antioxidant capacity, and vitamin C and phenolics content in fruits.
The main aim of the study was to evaluate the antioxidant activity and total polyphenols content in fifteen herbal plants, such as chamomile flower head, oak bark, St. John’s-wort herb, hawthorn flower, dog rose and elder fruits, lingonberry leaf, and many more. From the herbs both the water and methanolic extracts were prepared. The highest antioxidant activity and the highest polyphenols level were obtained for lingonberry leaves (1752.9 mg Trolox/g d.w. and 199.6 mg catechin/g d.w., respectively). The oak bark, St. John’s-wort herb, and flowers of hawthorn and elder were also a very valuable source of antioxidant compounds. It was demonstrated that, in contrary to water, methanol was more efficient solvent for extraction of antioxidant compounds from lingonberry leaf, lime flower, fruits of elder and doge rose, oak bark, flowers of hawthorn, St. John’s-wort herb and heartsease herb. Therapeutic activity of some examined herbs did not depend on antioxidant activity.
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