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Alkoholizm i neurobiologia farmakoterapii alkoholizmu

100%

Vetulani J.

Wszechświat

2013

tom 114

nr 01-03

Enzymatic oxidation of phthalazine with guinea pig liver aldehyde oxidase and liver slices: inhibition by isovanillin

100%

Panoutsopoulos G. I., Beedham C.

Acta Biochimica Polonica

2004

tom 51

nr 4

The enzymes aldehyde oxidase and xanthine oxidase catalyze the oxidation of a wide range of N-heterocycles and aldehydes. These enzymes are widely known for their role in the metabolism of N-heterocyclic xenobiotics where they provide a protective barrier by aiding in the detoxification of ingested nitrogen-containing heterocycles. Isovanillin has been shown to inhibit the metabolism of aromatic aldehydes by aldehyde oxidase, but its inhibition towards the heterocyclic compounds has not been studied. The present investigation examines the oxidation of phthalazine in the absence and in the presence of the inhibitor isovanillin by partially purified aldehyde oxidase from guinea pig liver. In addition, the interaction of phthalazine with freshly prepared guinea pig liver slices, both in the absence and presence of specific inhibitors of several liver oxidizing enzymes, was investigated. Aldehyde oxidase rapidly converted phthalazine into 1-phthalazinone, which was completely inhibited in the presence of isovanillin (a specific inhibitor of aldehyde oxidase). In freshly prepared liver slices, phthalazine was also rapidly converted to 1-phthalazinone. The formation of 1-phthalazinone was completely inhibited by isovanillin, whereas disulfiram (a specific inhibitor of aldehyde dehydrogenase) only inhibited 1-phthalazinone formation by 24% and allopurinol (a specific inhibitor of xanthine oxidase) had little effect. Therefore, isovanillin has been proved as an inhibitor of the metabolism of heterocyclic substrates, such as phthalazine, by guinea pig liver aldehyde oxidase, since it had not been tested before. Thus it would appear from the inhibitor results that aldehyde oxidase is the predominant enzyme in the oxidation of phthalazine to 1-phthalazinone in freshly prepared guinea pig liver slices, whereas xanthine oxidase only contributes to a small extent and aldehyde dehydrogenase does not take any part.

Wplyw tiuramu i disulfiramu na stan redox w komorkach fibroblastow pluca chomika chinskiego

85%

Grosicka E., Czeczot H., Skrzycki M., Szumilo M., Podsiad M., Rahden-Staron I.

Bromatologia i Chemia Toksykologiczna

2006

tom 39

nr 4

383-390

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Bromatologia i Chemia Toksykologiczna

1 Wszechświat

1 Beedham C.

1 Czeczot H.

1 Grosicka E.

1 Panoutsopoulos G. I.

1 Podsiad M.

1 Rahden-Staron I.

1 Skrzycki M.

1 Szumilo M.

1 Vetulani J.

1 2013

1 2006

1 2004

Wyniki wyszukiwania

Alkoholizm i neurobiologia farmakoterapii alkoholizmu

Enzymatic oxidation of phthalazine with guinea pig liver aldehyde oxidase and liver slices: inhibition by isovanillin

Wplyw tiuramu i disulfiramu na stan redox w komorkach fibroblastow pluca chomika chinskiego