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This study deals with the polyviewed expression of the altered contractility of the isolated ileum of the guinea-pig after ischemia/superfusion (I/S). Intestinal ischemia was produced by clamping the superior mesenteric artery for 40, 80 or 160 min. Ischemic and non-ischemic segments taken from the same guinea-pig were mounted for tension recording in organ baths and superfused (120 min) with an oxygenated Krebs-bicarbonate solution. Data were analyzed by means of the Polyview System software, which allows detecting simultaneously several events of one response. Histopathological changes in myenteric neurons were also examined. We found that ischemia in situ followed by superfusion in vitro (reoxygenation) severely reduces the spontaneous intestine contractile activity, and significantly decreases the maximal contractile response to ACh and to electrical field stimulation (EFS), the maximal rate of tension, and the sensitivity of the tissue to EFS. In addition, these ischemic intestines respond with a long-lasting contracture when electrical stimulation was started at supramaximal voltage. Functional alterations were time dependent. Neurons exhibited features of necrosis. These results provide clear evidence of detrimental effects of I/S on intestine contractile function. Digital analysis allows quantification of additional parameters important for evaluation of functional changes after I/S and of the degree of neuroprotection.
The anterior tibial artery is of great clinical relevance to vascular infrapopliteal surgery. The sources (origins), length and luminal diameter of the anterior tibial artery in 46 men and 30 women with Lerich syndrome were studied by means of radiological and digital methods. The results obtained were described by twoway analysis of variance (Multi-group ANOVA) for unpaired data — the means for six subtypes with regard to sex and side of the body, using the STATISTICA 5.5 program. The anterior tibial artery occurred most frequently (92.11%) as a terminal branch of the popliteal artery in its normal (IA: 87.5 %, IB: 2.63%) and high (IIA 1: 1.32%, IIA 2: 0.66%) division. In the remainder (7.89%), the anterior tibial artery arose from both the anterior tibioperoneal trunks (IC: 1.97%, IIB: 5.92%). The statistical analysis of the sources of the anterior tibial artery did not show gender differences. Symmetry of the left and right popliteal patterns was observed in the two most frequent subtypes: IA (r₁ = 0.80) and IIB (r₂ = 0.83). The anterior tibial artery was the longest (p = 0.02 for men, p = 0.04 for women) in subtype IIA 2. The greatest diameter of the anterior tibial artery was characteristic for a trifurcation (IB) and the smallest for subtype IIA 2 (p = 0.04). Both the length (p = 0.03) and luminal diameter (p = 0.04) of the anterior tibial artery in men were significantly greater than in women in all the popliteal subtypes observed. Morphometric parameters of the right and left anterior tibial artery showed no statistically significant differences. The anterior tibial artery was the predominant vessel in a trifurcation (IB) and in the two subtypes with an anterior tibioperoneal trunk (IC, IIB). These results have implications in vascular grafting below the knee.
A novel diagnostic method was adapted from studies in horses and humans to postmortal research on ostrich bones. The research made it possible to determine referential values in the method of digital analysis of a radiological image for the tibio-tarsal bone of the ostrich, following exposure to injuries. In the case of a computer-generated analysis of a radiological image, the optimal conditions for taking images were selected, along with their digital record and processing: 0.096 mm/pixel, 256 greyness level (8 bits), and scanning in BMP (a bitmap that is an open format of raster graphics, a block of bytes, which describes an image pixel by pixel). The research was conducted on the rectangular-shaped (160 x 320 points) spongy bone taken about 80 mm below the articular surface near the proximal metaphysis. The research allowed defining parameters of the spongious matrix of the tibio-tarsal bone of healthy 14-month-old ostriches. The average number of trabeculae on lines was 10.41 mm² for both sexes, the average volume was 2.21 %, the density of the trabeculation (the percentage of the surface covered with trabeculae) was 47.44 % for both sexes and the average width of the trabeculae was 0.23 mm. On the basis of the conducted calculations, it is possible to draw a conclusion that the average number of radiological trabeculae, the volume of the trabeculae, and their density do not differ significantly for cocks and hens.
W artykule opisano korzystne cechy fluorescencji warstwy barwników uporządkowanych na powierzchni roztworów. Przedstawiono warunki tworzenia się takiego układu fizycznego oraz schemat układu pomiarowego odpowiedniego do rejestracji fluorescencji w nim powstającej. Przedstawiono także założenia programu komputerowego zastosowanego do ilościowej analizy kształtów wykresów widm synchronicznych fluorescencji powierzchniowej próbek miodów.
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