Znaleziono wyników: 3

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: dehydroepiandrosterone sulphate

Sortuj według:

Ogranicz wyniki do:

Excitatory neurosteroids attenuate apoptotic and excitotoxic cell death in primary cortical neurons

100%

Leskiewicz M., Jantas D., Budziszewska B., Lason W.

Journal of Physiology and Pharmacology

2008

tom 59

nr 3

457-475

Some neurosteroids show neuroprotective action in in vitro and in vivo studies, but their interaction with apoptotic/necrotic processes has been only partially unraveled. The aim of the present study was to examine the effect of dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (DHEAS), pregnenolone (PGL) and allopregnanolone (Allo) on staurosporine-, glutamate-, and NMDA-induced damage in primary cortical neuronal culture. DHEA, DHEAS and PGL (0.1 and 1 µM) inhibited the staurosporine-evoked LDH release and decreased the number of apoptotic cells as shown by Hoechst`s staining, whereas Allo was without effect. The neurosteroids affected neither the staurosporine-evoked changes in caspase-3 activity nor the decrease in mitochondrial membrane potential. It was also shown that protective effects of DHEA, DHEAS and PGL against staurosporine-induced LDH release were attenuated by extracellular signal-regulated kinase (ERK) - mitogen-activated protein kinase (MAPK) inhibitor – PD 98059 (5 µM) but not by phosphatidylinositol-3-kinase (PI3-K) inhibitors such as LY 294002 (1 µM) or wortmannin (10 nM). The involvement of ERK2-MAPK in protective effects of neurosteroids was confirmed by Western blot study. Further study demonstrated that glutamate-induced cell damage was attenuated by DHEA, DHEAS, and PGL, but not by Allo. None of the steroids influenced NMDA-induced LDH release. The results of the present in vitro studies suggest that excitatory neurosteroids DHEA, DHEAS and PGL at physiological concentrations participate in the inhibition of cortical neuronal degeneration elicited by staurosporine and glutamate, whereas the most potent positive modulator of GABAA receptor - Allo - has no effect. Moreover, neurosteroids appear to attenuate the staurosporine-induced cell damage in a caspase-3 independent way and their neuroprotective mechanism of action involves the increase in ERK-MAPK phosphorylation.

Hormone concentration, metabolic disorders and immunoexpression of androgen and estrogen--alpha receptors in men with benign prostatic hyperplasia and testosterone deficiency syndrome

80%

Ryl A., Rotter I., Slojewski M., Dolegowska B., Grabowska M., Baranowska-Bosiacka I., Laszczynska M.

Folia Histochemica et Cytobiologica

2015

tom 53

nr 3

Sulphohydrolase activities in subcellular fractions of human placenta - estimation with natural and synthetic substrates

80%

Gniot-Szulzycka J., Wojczuk B.

Acta Universitatis Nicolai Copernici. Biologia

1997

tom 52[96A]

Ograniczanie wyników

1 Acta Universitatis Nicolai Copernici. Biologia

1 Folia Histochemica et Cytobiologica

1 Journal of Physiology and Pharmacology

1 Baranowska-Bosiacka I.

1 Budziszewska B.

1 Dolegowska B.

1 Gniot-Szulzycka J.

1 Grabowska M.

1 Jantas D.

1 Lason W.

1 Laszczynska M.

1 Leskiewicz M.

1 Rotter I.

1 Ryl A.

1 Slojewski M.

1 Wojczuk B.

1 2015

1 2008

1 1997

Wyniki wyszukiwania

Excitatory neurosteroids attenuate apoptotic and excitotoxic cell death in primary cortical neurons

Hormone concentration, metabolic disorders and immunoexpression of androgen and estrogen--alpha receptors in men with benign prostatic hyperplasia and testosterone deficiency syndrome

Sulphohydrolase activities in subcellular fractions of human placenta - estimation with natural and synthetic substrates