Wyniki wyszukiwania

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Genistein and daidzein affect in vitro steroidogenesis but not gene expression of steroidogenic enzymes in adrenals of pigs

100%

Kaminska B., Czerwinska J., Wojciechowicz B., Nynca A., Ciereszko R.

Journal of Physiology and Pharmacology

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2014

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tom 65

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nr 1

2

In vitro effects of genistein and daidzein on the activity of adrenocortical steroidogenic enzymes in mature female pigs

100%

Kaminska B., Ciereszko R., Kiezun M., Dusza L.

Journal of Physiology and Pharmacology

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2013

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tom 64

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nr 1

3

Effects of phytoestrogen daidzein and estradiol on steroidogenesis and expression of estrogen receptors in porcine luteinized granulosa cells from large follicles

80%

Nynca A., Jablonska O., Slomczynska M., Petroff B. K., Ciereszko R. E.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 2

95-105

The aims of the study were to compare the in vitro effects of daidzein or 17ß-estradiol (E2) on: 1) progesterone (P4) secretion by luteinized granulosa cells harvested from large porcine follicles, as well as 2) estrogen receptor and ß (ER and ERß) mRNA and protein expression in the cells. In addition, the effect of daidzein on E2 secretion and viability of the granulosa cells was examined. We found that basal and gonadotropin-stimulated P4 secretion were inhibited in granulosa cells cultured in the presence of daidzein either for 24 or 48 hours. In contrast to daidzein, E2 reduced P4 secretion only during 24-hour cell cultures increasing it during longer cultures. Daidzein did not affect E2 secretion by granulosa cells. The expression of ER and ERß mRNA, as well as ERß protein, was up-regulated by daidzein but unaffected by E2. To conclude, the soy estrogen daidzein acts directly on the porcine ovary to decrease progesterone production and to increase expression of ERß mRNA and protein. Daidzein actions in porcine luteinized granulosa cells differ from those of estradiol and it may suggest disadvantageous effects of the phytoestrogen on reproductive processes in females.

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Effect of daidzein, a soy isoflavone, on bone metabolism in Cd-treated ovariectomized rats

80%

Om A. S., Shim J. Y.

Acta Biochimica Polonica

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2007

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tom 54

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nr 3

This study compared the ability of daidzein, a soy isoflavone, with that of 17 β-estradiol to prevent bone loss in cadmium (Cd)-exposed ovariectomized (OVX) rats during growth. Four week-old female Wistar rats were randomly assigned to five treatment groups of 9 rats each, either (1) sham-operated (SH); (2) OVX and placed on experimental diets (OVX); (3) OVX fed 50 ppm of CdCl2 (OVX-Cd); (4) OVX fed 50 ppm of CdCl2 and 10 µg of daidzein per kg of body mass (OVX-CD-D); or (5) OVX fed 50 ppm of CdCl2 and 10 µg of estrogen per kg of body mass (OVX-CD-E). All rats were given free access to AIN-76 modified diet and drinking water, with or without Cd, for 8 weeks. The OVX groups gained more (P < 0.05) body mass than the SH group. Femoral mass was increased by feeding daidzein and estradiol, whereas femoral length was not (P > 0.05) significantly different among groups. Femoral breaking force was not significantly different among groups, however, femoral BMD was significantly lower in OVX-Cd than in the SH and OVX groups. Morphologically proliferative cartilage and hypertrophic cells in femur showed normal distribution in OVX-Cd-D and OVX-Cd-E groups unlike those in OVX-Cd group. These findings suggest that Cd-OVX-induced osteopenia or osteoporosis probably results from an increase in bone turnover.

Ograniczanie wyników

3 Journal of Physiology and Pharmacology

1 Acta Biochimica Polonica

2 Ciereszko R.

2 Kaminska B.

2 Nynca A.

1 Ciereszko R. E.

1 Czerwinska J.

1 Dusza L.

1 Jablonska O.

1 Kiezun M.

1 Om A. S.

1 Petroff B. K.

1 Shim J. Y.

1 Slomczynska M.

1 Wojciechowicz B.

1 2014

1 2013

1 2009

1 2007

Genistein and daidzein affect in vitro steroidogenesis but not gene expression of steroidogenic enzymes in adrenals of pigs

In vitro effects of genistein and daidzein on the activity of adrenocortical steroidogenic enzymes in mature female pigs

Effects of phytoestrogen daidzein and estradiol on steroidogenesis and expression of estrogen receptors in porcine luteinized granulosa cells from large follicles

Effect of daidzein, a soy isoflavone, on bone metabolism in Cd-treated ovariectomized rats