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Rola czynników troficznych w rozwoju i regeneracji mięśni szkieletowych

100%

Ciecierska A., Sadkowski T., Motyl T.

Medycyna Weterynaryjna

2019

tom 75

nr 12

The process of skeletal muscle development is regulated by many biologically active factors, which are responsible for stimulating the proliferation and differentiation of muscle cells. Biologically active factors function in paracrine, autocrine and endocrine manner to control myogenesis. The main regulators include hormones, growth and differentiation factors, as well as cytokines. The process of skeletal muscle regeneration associated with the activation of satellite cells for their proliferation and differentiation requires the involvement of many growth factors secreted by the surrounding tissue, including inflammatory cells, blood vessels and damaged muscle fiber, as well as extracellular matrix. A number of trophic factors regulating the activity of satellite cells during muscle regeneration have been identified, e.g. fibroblast growth factors, transforming growth factors-β, insulin-like growth factors, hepatocyte growth factor, tumor necrosis factor-α, interleukin-6. These factors are responsible for maintaining a balance between the processes of proliferation and differentiation of satellite cells in order to restore the proper architecture and functioning of muscle tissue.

Ekspresja genow dla NP-I-OT i PGA w komorkach lutealnych i warstwy ziarnistej pecherzyka jajnikowego oraz czynnika LIF w komorkach endometrium krowy pod wplywem DDT i jego metabolitow

100%

Mlynarczuk J., Wrobel M., Kotwica J.

Medycyna Weterynaryjna

2008

tom 64

nr 04B

579-582

Ovarian oxytocin (OT) and endometrial leukemia inhibitory factor (LIF) are involved in estrous cycle regulation and implantation of the blastocyst in cows. For this reason the authors investigated the effect of DDT and its metabolites (DDE), known as environmental pollutants, on the expression of genes involved in OT and LIF synthesis. Granulosa from follicles (1 cm in diameter), luteal and endometrial cells from cows on days 8-12 of the estrous cycle were treated for 6 h with DDT, p,p’-DDE, o,p’-DDE and technical mixture (MIX) of DDE isomers (10 ng/ml each). Obtained RNA was reverse transcribed and cDNA was amplified by PCR using primers for genes of NP-I/OT, PGA, LIF and G3PDH as a reference gene. DDT and DDE in granulosa cells and DDE in luteal cells increased (P < 0.05) the expression of the NP-I/OT gene, while DDE in granulosa cells and MIX in luteal cells increased the expression of the PGA gene (P < 0.05). In contrast, p,p’-DDE and MIX reduced (P < 0.05) the LIF expression in endometrial cells. Obtained data allow the authors to assume that DDE and its metabolite impairs regulation of the estrous cycle, affecting OT and LIF synthesis on the genomic level.

Ograniczanie wyników

2 Medycyna Weterynaryjna

1 Ciecierska A.

1 Kotwica J.

1 Mlynarczuk J.

1 Motyl T.

1 Sadkowski T.

1 Wrobel M.

1 2019

1 2008

Wyniki wyszukiwania

Rola czynników troficznych w rozwoju i regeneracji mięśni szkieletowych

Ekspresja genow dla NP-I-OT i PGA w komorkach lutealnych i warstwy ziarnistej pecherzyka jajnikowego oraz czynnika LIF w komorkach endometrium krowy pod wplywem DDT i jego metabolitow