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Phylogeography analysis and molecular evolution patterns of the nematode parasite Heligmosomum mixtum based on mitochondrial DNA sequences

100%

Sakka H., Henttonen G., Baraket G., Amel S.-H., Michaux J.

Acta Parasitologica

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2015

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tom 60

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nr 1

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Paramecium tredecaurelia: a unique non-polymorphic species of the P. aurelia spp. complex (Oligohymenophorea, Ciliophora)

67%

Przybos E., Tarcz S., Surmacz M., Sawka N., Fokin S. I.

Acta Protozoologica

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2013

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tom 52

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nr 4

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Mitochondrial cytochrome b sequences resolve the taxonomy of field mice (Apodemus) in the western Balkan refugium

67%

Krystufek B., Luznik M., Buzan E. V.

Acta Theriologica

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2012

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tom 57

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nr 1

Apodemus sylvaticus stankovici, described from the topographically rough landscape of the western Balkan glacial refugium, was recently proposed as being either a junior synonym of Apodemus flavicollis or a species on its own right. To untangle this taxonomic vagueness, we sequenced complete cytochrome b gene in 28 field mice collected at 12 locations in the mountains of Bosnia and Herzegovina, Montenegro, western Macedonia and northern Greece. Samples yielded 27 new haplotypes which clustered into two distinct groups. One of these clades also included the reference haplotype of A. flavicollis, while another cluster emerged as being identical with the reference sample for A. sylvaticus. As is common in Apodemus, both species retrieved in our analysis were characterized by low levels of intraspecific variation (0.4–0.9%) as opposed to a high level of differentiation between them (8.0–10.0%); therefore, the taxonomic classification of our material was without doubt. We found no evidence regarding the presence of an additional cryptic species in the mountains of the western Balkans. The very similar values of genetic variability in the two species imply their common evolutionary history of a long-term coexistence in the western Balkan refugium.

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Further studies of mitochondrial genome variability in Ponto-Caspian Proterorhinus species (Actinopterygii: Perciformes: Gobiidae) and their taxonomic implications

67%

Sorokin P. A., Medvedev D. A., Vasil'ev V. P., Vasil'eva E. D.

Acta Ichthyologica et Piscatoria

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2011

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tom 41

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nr 2

Background. A new taxonomy of tubenose gobies of the genus Proterorhinus has been proposed recently. However, some of taxonomic hypotheses have not been confirmed by recent genetic studies or seem not to correspond with ecological features and genetic differentiation of these fishes. The aim of this paper was to test the new taxonomy through a phylogeographic analysis of the fish from 15 sites in Southern Ukraine and Russia, through analyzing the genotypes deposited in the GenBank and to define the range of those species. Materials and methods. Fin clips were sampled from 52 specimens of tubenose gobies collected from 2003 up to 2009 in 15 localities of the Ponto-Caspian basin. Genomic DNA for amplification of mitochondrial cyt b gene fragment (about 408 bp) was extracted using the Diatom®DNA Prep 100. The haplotype MedianJoining Network was constructed using the Network 4.5.1.0. software. Results. Tubenose gobies from the Black Sea basin are represented by two euryhaline species historically distributed in different areas: P. semilunaris in the north-western part of the Black Sea basin (and possibly eastern Aegean basin) and P. marmoratus in the north-eastern part. The hypothesis about freshwater and marine Black Sea Proterorhinus species seems to be not probable. The validity of endemic Crimean P. tataricus is not confirmed, several independent findings presume it to be conspecific with P. marmoratus. Some Crimean rivers and reservoirs are populated by P. semilunaris which could spread from the Dnieper River basin through the Severo-Krymskyi channel or may be accidentally introduced. The populations from the Caspian Sea and Sea of Azov basins represent the distinct evolutionary lineage of tubenose goby. But their taxonomic state should be analyzed by further molecular genetic studies of a wide scope of Caspian samples. Conclusion. The present data on the variability of the mitochondrial cyt b gene fragment in different tubenose goby populations result in revision of some previous taxonomic hypotheses in this fish group. At the same time further molecular genetic researches of Caspian populations seem to be the main tool for future development of taxonomic conclusions.

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Genetic variation and molecular evolution of endangered Kozlov's pika (Ochotona koslowi Buchner) based on mitochondrial cytochrome b gene

67%

Lin G., Ci H., Thirgood S. J., Zhang T., Su J.

Polish Journal of Ecology

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2010

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tom 58

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nr 3

Kozlov’s pika is a rare and endangered lagomorph species with a limited distribution in the southern Kunlun Mountains in western China. Because of its endangered status, Kozlov’s pika is considered a priority species for research and conservation action. Genetic variation and molecular evolution of the Kozlov’s pika were studied based on a total of 14 individuals from four locations along the eastern boundary between Xinjiang and Tibet province (35.20–36.48°N, 86.08–83.04°E) on extremely high elevation (usually over 4800 m a.s.l.). The density of local populations was about 3–4 per ha, living in a typical alpine desert grassland habitat. The complete mitochondrial cytochrome b (cytb) gene was amplified and sequenced. Based on the cytb gene sequences the genetic variation and molecular evolution were analyzed. Unexpected high haplotype diversity (0.956 ± 0.045) but low nucleotide diversity (0.00537 ± 0.00126) was found, indicating past demographic expansion. Significant partitioning of variance (P <0.01) among populations (46.7%), and within populations (53.3%), indicating low level of genetic differentiations among local populations. Our results gave an optimistic survival status of Kozlov’s pika at the genetic level. Bayes Empirical Bayes analysis with model M2a and M8 detected three positively selected amino acid sites at the significance level of 0.05. The mutant types with either or both of the mutations aspartic acid to asparagine and glutamic acid to lysine had higher isoelectric point values. We suggested these mutant types might have biological significance to help individuals to adapt to the extremely high elevation habitats.

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A taxonomical re-evaluation of the Valais chromosome race of the common shrew Sorex araneus [Insectivora: Soricidae]

67%

Brunner H., Lugon-Moulin N., Balloux F., Fumagalli L., Hausser J.

Acta Theriologica

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2002

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tom 47

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nr 3

The common shrew Sorex araneus Linnaeus, 1758 is subject to intense chromosomal polymorphism. About 65 chromosome races are presently known. One of these chromosome races (the Valais race) is karyologically, morphologically, biochemically, and genetically clearly distinct from all other chromosome races of the species. Recent studies of hybrid zones between the Valais race and other chromosome races in the Swiss and French Alps add further strong evidence for the specific taxonomic status of the Valais race. Chromosomes and diagnostic protein markers reveal sharp frequency clines and strong heterozygote deficits. In one hybrid zone, the maintenance of the strong genetic differentiation of the hybridizing taxa was confirmed by a study with autosomal microsatellites indicating minimal gene flow. A microsatellite marker on the Y-chromosome showed complete absence of male mediated gene flow suggesting hybrid male sterility. To clarify the taxonomic status of this taxon, additional analyses were conducted. A morphometric analysis of the mandible indicated the Valais race is morphologically as distinct from neighbouring chromosome races of S. araneus as from other related Sorex species. In a phylogeny based on complete mitochondrial DNA cytochrome b gene sequences, the Valais race clearly appears as the sister taxon to all other races of S. araneus. Therefore, the chromosome race Valais of S. araneus herein is elevated to specific status and the name Sorex antinorii Bonaparte, 1840 is applied.

Ograniczanie wyników

Phylogeography analysis and molecular evolution patterns of the nematode parasite Heligmosomum mixtum based on mitochondrial DNA sequences

Paramecium tredecaurelia: a unique non-polymorphic species of the P. aurelia spp. complex (Oligohymenophorea, Ciliophora)

Mitochondrial cytochrome b sequences resolve the taxonomy of field mice (Apodemus) in the western Balkan refugium

Further studies of mitochondrial genome variability in Ponto-Caspian Proterorhinus species (Actinopterygii: Perciformes: Gobiidae) and their taxonomic implications

Genetic variation and molecular evolution of endangered Kozlov's pika (Ochotona koslowi Buchner) based on mitochondrial cytochrome b gene

A taxonomical re-evaluation of the Valais chromosome race of the common shrew Sorex araneus [Insectivora: Soricidae]