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F₁, F₂ populations as well as F₃ and F₄ wheat lines coming from more than 300 cross combinations have been used for androgenic wheat studies and more than 5000 haploid plants were produced. Promising doubled haploid lines were tested together with lines produced in the conventional way in yield trials. The two best doubled haploid lines overyielded the standard. On the basis of results related to the general combining ability it is advisible to cross parents with a good combining ability for characters such as high percentage of responding anthers and embryo/callus induction with parents indicating a good combining ability for green plant production. In this way ideal lines can be obtained for doubled haploid production.
Three steps of multi-stage selection for more vigorous seminal roots were done in two cross combinations of oat. The total length of roots (TRL) was measured in seedlings of the F₂, F₃ and F₄ generations grown in filter-paper rollers. On average, the finally selected F₅ (or F₆) progenies distinguished by 7-11% improved rooting ability than their midparents. Transgressive forms were selected only in one cross combination. Coefficients of realized heritability for TRL were low and ranged from 0.08 to 0.42 indicatinga relatively high proportion of non-additive gene effects in the variance of TRL. Tolerance of the F₆-progenies to water and nutrient limitations was evaluated in pot and field experiments. Correlated selection effects were dependent upon initial cross combination. Only progenies from one cross combination exhibited an improved drought tolerance at the 8-9 Feekes' stage. The both enhanced rooting and decreased S : R ratio of the F₆-progenies correlated with their improved tolerance to reduced P supply at the 4-leaf stage. However, no significant consistence occurred between indices of the tolerance to reduced nutrition at the vegetative growth and those at field maturity. Performed root selection identified more frequently high yielding genotypes with less stable grain yield. No progenies were obtained that outperform their parents under low-input conditions. It was emphasized that root selection should be accompanied by selection for integrated response components.
The valuable genes of Aegilops biuncialis, Ae. ovala, Ae. kotschyi, and Ae. variabilis were transferred to rye, by crossing Aegilops-rye amphiploids with tetraploid and diploid substitution rye. The C-banded karyotype of the BC₁ and BC₂ generations of amphiploids with 4x substitution rye and BC₁ with 2x substitution rye showed great variation in chromosome number and composition. In the BC₁ generation of amphiploids with 4x and 2x substitution rye, seed set success rate and germination rate varied depending on origin. However, plant sterility in all cross combinations of amphiploids with 4x and 2x substitution rye resulted in their elimination from further experiments in the BC₃ and BC₂ generations, respectively. In backcrosses of 4x substitution rye with amphiploids Ae. variabilis x rye 4x, fertile 4x rye plants containing Aegilops chromatin were produced in the BC₂ generation.
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