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1

Amplification of DNA markers from scat samples of the least weasel Mustela nivalis nivalis

100%
Wang R., Painter J. N., Hanski I.
Acta Theriologica
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2002
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tom 47
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nr 4
To test the feasibility of using field-collected scats as a source of DNA in the study of the least weasel Mustela nivalis nivalis Linnaeus, 1766, DNA was extracted from scat samples collected from captive weasels using a modified extraction protocol. Using universal primers, the control region of the mitochondrial genome was successfully amplified from scat-extracted DNA. This amplification resulted in two products; one equivalent in size and sequence to the product obtained from tissue-extracted weasel DNA, and the other slightly larger and equivalent in size and sequence to the domestic house mouse Mus musculus, the food source of the captive weasels. This demonstrates the reliability of DNA extraction from scats, as well as the possibility, under favourable circumstances, of identifying the prey species from the same samples. In addition, we attempted to amplify microsatellite loci from both tissue and scat-extracted DNA using six primer pairs designed for other mustelids, the American mink Mustela vison and the wolverine Gulo gulo. While three loci, Mvi57 (American mink), Ggu216 and Ggu234 (wolverine), were found to be polymorphic in the least weasel, amplification of these loci from the scat extracted DNA was only successful for approximately half of the samples. Although further work is needed, the present results suggest that it is possible to use scats as a source of DNA in field studies of the least weasel.
2

Sequence polymorphism in a novel noncoding region of Pacific oyster mitochondrial DNA

100%
Aranishi F., Okimoto T.
Journal of Applied Genetics
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2005
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tom 46
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nr 2
Nucleotide sequence polymorphism in a 641-bp novel major noncoding region of mitochondrial DNA (mtDNA-NC) of the Pacific oyster Crassostrea gigas was analysed for 29 cultured individuals within the Goseong population. A total of 30 variable sites were detected, and the relative frequency of nucleotide alteration was determined to be 4.68. Alterations were mostly single nucleotide substitutions. Transition, transversion, both transition and transversion, and both transversion and nucleotide deletion were observed at 18, 9,2 and 1 sites, respectively. Among 29 specimens, 22 haplotypes were identified, and pairwise genetic diversity of haplotypes was calculated to be 0.988 from multiple sequence substitutions using the two-parameter model. A phylogenetic tree, obtained for haplotypes by the neighbor-joining method, showed a single cluster of linkages. The cluster comprised 11 haplotypes associating with 14 specimens, while the other 11 haplotypes associating with 15 specimens were scattered. This mtDNA-NC presenting a high nucleotide sequence polymorphism is a potential mtDNA control region. It therefore can serve as a genetic marker for intraspecies phylogenetic analysis of the Pacific oyster and is more useful than the less polymorphic mtDNA coding genes.
3

The genetic analysis of trout skeletal remains: how should the potential of ancient fish DNA be utilized?

80%
Ciesielski S., Brzuzan P.
Archives of Polish Fisheries
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2003
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tom 11
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nr 2
DNA can be retrieved from preserved biological remains; this provides scientists with an opportunity to directly measure molecular evolution over large periods of time. This means that the taxonomic affinity of extinct taxa can be ascertained by studying archival material found at archaeological sites. This paper describes a molecular approach which was used to identify the taxonomical position of a fish whose bones came from an archaeological site in Wolin in northern Poland. Ancient DNA was successfully extracted, and a fragment of about 350 bp from the mitochondrial control region was amplified with PCR and sequenced. The molecular analysis based on ancient and modern sequences of the mitochondrial control region proved that the archival bone was a component of a Salmo trutta skeleton. However, the mtDNA sequence examined was more similar to the haplotype of trout from the Adriatic Sea basin than to fish inhabiting the waters of the southern Baltic Sea coast.
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