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Conformation polymorphism in myogenin gene in pigs

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Nowak Z., Hermanowicz-Swierczek A., Charon K. M., Kulisiewicz J.

Animal Science Papers and Reports

2003

tom 21

nr 4

Conformation polymorphism (SSCP) in exons 1-3 of myogenin gene was analysed in two groups of fatteners, both out of crossbred sows (Polish Large White × Polish Landrace) sired by Duroc (group I) or Duroc × Pietrain (group II) boars. The total DNA was isolated from a whole blood using phenol/chloroform extraction. Amplifications of exons was carried out using PCR method and primers designed by computer software “Primer 3” (www.genome.wi.mit.edu).Exon 1 showed low polymorphism with two SSCP patterns: A (two bands) in 98% of group I and 94% of group I, and E (three bands) in 2% of group I and 6% of group II fatteners. Exon 2 in all fatteners was found monomorphic. Exon 3 showed high polymorphism with four SSCP patterns:A, B, C and D (one to three bands). Most frequently observed was pattern A (88% in group I and 82% in group II), while the remaining patterns occurred in 2-10% of fatteners. Sequence analysis of conformation patterns did not show any mutation sites.

Ograniczanie wyników

1 Animal Science Papers and Reports

1 Charon K. M.

1 Hermanowicz-Swierczek A.

1 Kulisiewicz J.

1 Nowak Z.

1 2003

Wyniki wyszukiwania

Conformation polymorphism in myogenin gene in pigs