At the first stage of the study the experimental material included 300 clutching eggs of Hubbard Flex chickens. The eggs were divided into three groups: control (without injection in ovo), Nano50 (in ovo injection of colloidal copper nanoparticles) and NanoCuSO4 (in ovo injection of colloidal nanoparticles of copper sulphate). Experimental solutions were administered by in ovo injection using a sterile needle 0.3 mm as follows: Nano50 group - colloid of copper nanoparticles (concentration: 50 ppm), and NanoCuSO4 group - colloid of copper sulfate (concentration: 50 ppm), to the air cell of the egg. The eggs were incubated under standard conditions. After hatching, 50 chicks were selected from each group for 42-day rearing. Birds were fed standard complete feed mixtures for broilers. On the last day of rearing (day 42), 12 females and 12 males were selected from each group and their blood was sampled for assays of hematological and biochemical markers. Hematological analyses included determinations of: WBC, RBC, Hb, heterophils, lymphocytes, monocytes, eosinophils and basophils, whereas biochemical analyses included assays of the following markers in blood serum: glucose, cholesterol, triglycerides, HDL-cholesterol, urea, calcium, magnesium, phosphorus, iron, ASPAT, and ALAT. The use of copper nanoparticles evoked an increase in blood levels of RBC, HGB, HTC, heterophils, monocytes and basophils. In addition, in blood serum in contributed to reduced concentrations of glucose and cholesterol and increased levels of selected microelements: calcium, phosphorus and iron.
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