Colchicine treatment was done to obtain the tetraploid forms of chamomile rich in α-bisabolol. The following morphological modifications of organs of chamomile plants were observed i.e.: enlarged, thicken, dark green leaves, wide, accreted, flattened and convex-like inflorescences. 1,0% colchicine in agar gave the most visible, morphological modifications and all new tetraploid strains descended from this combination.