Wyniki wyszukiwania

1

Structural domains of plant nuclear DNA as a constitutive component of the topoisomerase II-DNA complex

100%

Solovyan V. T., Andreyev I. O.

Acta Biochimica Polonica

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1995

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tom 42

|

nr 2

The treatment of agarose embedded plant nuclei by strong protein dénaturants was demonstrated to result in discrete self-fragmentation of intact nuclear DNA. The set of resultant DNA cleavage products involves two main types of DNA fragments sized about 50-100 kb and 300-500 kb, being of the same type in various eukaryotic representatives. The pattern of ordered DNA fragmentation has been shown to be similar both in intact nuclei and in histone-depleted ones thus suggesting that the observed DNA fragments represent preexisting DNA structural domains, corresponding to the higher levels of chromatin folding. The topoisomerase II-speeific poison teniposide (VM-26) has been shown to increase the ordered DNA cleavage while the conditions stimulating the topoisomerase II-mediated reverse reaction lead to the reassociation of the cleaved DNA domains. The data presented suggest that the nuclear DNA structural domains are involved in functioning of the topoisomerase II/DNA complex, the main property of which is its ability to mediate the cleavage/ /reassociation reactions.

2

Oocyte maturation in the presence of randomly pooled follicular fluid increases bovine blastocyst yield in vitro

86%

Mohammed A. A., Karasiewicz J., Papis K., Modlinski J. A.

Journal of Animal and Feed Sciences

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2005

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tom 14

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nr 3

501-512

3

Specificity and mechanism of the cleavages induced in yeast tRNAPhe by magnesium ions

86%

Marciniec T., Ciesiolka J., Wrzesinski J., Wiewiorowski M., Krzyzosiak W. J.

Acta Biochimica Polonica

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1989

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tom 36

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nr 3-4

4

Karyomere formation in cleaving eggs of Tetrodontophora bielanensis [Collembola]

86%

Klag J., Jura C., Krzysztofowicz A., Kisiel E.

Folia Biologica

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1995

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tom 43

|

nr 1-2

5

Caspase-mediated cleavage of the chromatin-binding domain of lamina-associated polypeptide LAP2alpha

86%

Gotzmann J., Vlcek S., Foisner R.

Cellular and Molecular Biology Letters

|

2000

|

tom 05

|

nr 2

6

Cleavage of prothrombin bound in immune complexes results in high thrombin enzymatic activity

86%

Pajdak W., Radwan J., Guzik T. J.

Journal of Physiology and Pharmacology

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2004

|

tom 55

|

nr 2

Thrombin plays a pivotal role in blood clotting as well as in the regulation of vascular remodeling and oxidative stress. Recent evidence suggests that auto-antibodies directed against prothrombin, may play an important role in the pathogenesis of atherosclerosis. It is however not clear, if prothrombin bound in an immune complex retains its clotting and regulatory properties or acts solely by increasing vascular inflammation. In order to answer this question, we used a newly developed stain for the detection of thrombin activity of such complexes. Plasma and serum samples were subjected to rocket immunoelectrophoresis in an anti-prothrombin antiserum containing agarose gel. Gel plates, covered with a nitrocellulose membrane were soaked with chromogenic thrombin substrate. The product of thrombin activity was diazotized to red azo dye bound to nitrocellulose. Activity stain revealed barely discernible rockets in plasma, but heavily stained ones in serum. Pre-incubation with trypsin enhanced activity of immunoprecipitates deriving from plasma, but not from serum. Densitometric analysis showed, that the trypsin-enhanced activity in plasma derived immune complexes was twice as high as in serum derived immunoprecipitates. Thrombin active centre is not blocked by anti-prothrombin antiserum allowing to retain thrombin activity. Moreover, prothrombin in immunoprecipitate is readily cleaved by proteolytic enzymes. This cleavage could potentially be enhanced by antibody binding, although these results need to be confirmed using different antibodies.

7

Predetermination of cytokinesis and transition from holoblastic to partial superficial cleavage in early embryonic development of Tetrodontophora bielanensis [Collembola]

86%

Klag J., Krzysztofowicz A., Jura C., Kisiel E.

Folia Biologica

|

2000

|

tom 48

|

nr 3-4

8

Holoblastic early cleavage of Tetrodontophora bielanensis [Collembola] eggs, with special reference to its irregularity

86%

Klag J., Jura C., Krzysztofowicz A., Kisiel E.

Folia Histochemica et Cytobiologica

|

1999

|

tom 37

|

nr 3

9

Stereological analysis of mitochondria in embryos of Rana temporaria and Bufo bufo during cleavage

86%

Romek M., Krzysztofowicz E.

Folia Histochemica et Cytobiologica

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2005

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tom 43

|

nr 1

10

The level of non-hydrophobic lipids in pig embryos during cleavage

72%

Romek M., Gajda B., Weber P., Krzysztofowicz E., Smorag Z.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

|

nr 1

11

Utilization of yolk platelets during early embryonic development of Rana temporaria and Bufo bufo

72%

Romek M., Krzysztofowicz E.

Folia Histochemica et Cytobiologica

|

2001

|

tom 39

|

nr 3

12

Scanning electron microscopy of the egg surface of the common toad, Bufo bufo, following fertilization and during first cleavage

72%

Semik D., Kilarski W.

Folia Histochemica et Cytobiologica

|

1998

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tom 36

|

nr 1

13

Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites

58%

Kur J.

Acta Microbiologica Polonica

|

1993

|

tom 42

|

nr 2

14

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Cleavage in Ixodes ricinus [L.] [Acari: Ixodidae]

58%

Jasik K.

Wiadomości Parazytologiczne

|

2006

|

tom 52

|

nr 4

The author analyzed the first 5 days of embryonic development of Ixodes ricinus. The cleavage takes 4 days, being terminated on the 5th day of embryogenesis, when the cells start to invaginate and differentiate. The karyomeres play a role in the initial mitotic divisions. Cellularization occurs on the 2nd and 3rd day of embryogenesis. The blastoderm cells maintain their potential for division, and as from the 5th day cellular differentiation starts.

15

Rana esculenta developmental syndrome: fates of abnormal embryos from the first cleavage until spontaneous death

58%

Ogielska M.

Zoologica Poloniae

|

1994

|

tom 39

|

nr 3-4

16

Dynamic changes in the tubulin cytoskeleton during oogenesis and early development in the anuran amphibian Xenopus laevis [Daudin]

58%

Palecek J., Ubbels G. A.

Folia Histochemica et Cytobiologica

|

1997

|

tom 35

|

nr 1

Ograniczanie wyników

Structural domains of plant nuclear DNA as a constitutive component of the topoisomerase II-DNA complex

Oocyte maturation in the presence of randomly pooled follicular fluid increases bovine blastocyst yield in vitro

Specificity and mechanism of the cleavages induced in yeast tRNAPhe by magnesium ions

Karyomere formation in cleaving eggs of Tetrodontophora bielanensis [Collembola]

Caspase-mediated cleavage of the chromatin-binding domain of lamina-associated polypeptide LAP2alpha

Cleavage of prothrombin bound in immune complexes results in high thrombin enzymatic activity

Predetermination of cytokinesis and transition from holoblastic to partial superficial cleavage in early embryonic development of Tetrodontophora bielanensis [Collembola]

Holoblastic early cleavage of Tetrodontophora bielanensis [Collembola] eggs, with special reference to its irregularity

Stereological analysis of mitochondria in embryos of Rana temporaria and Bufo bufo during cleavage

The level of non-hydrophobic lipids in pig embryos during cleavage

Utilization of yolk platelets during early embryonic development of Rana temporaria and Bufo bufo

Scanning electron microscopy of the egg surface of the common toad, Bufo bufo, following fertilization and during first cleavage

Use of IHF - mediated Achilles' heel cleavage [IHF-AC] method for mapping ihf sites

Cleavage in Ixodes ricinus [L.] [Acari: Ixodidae]

Rana esculenta developmental syndrome: fates of abnormal embryos from the first cleavage until spontaneous death

Dynamic changes in the tubulin cytoskeleton during oogenesis and early development in the anuran amphibian Xenopus laevis [Daudin]