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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Phylogenetic inference of Ericales based on plastid genomes and implication of cp-SSRs

100%
Hazra A., Das S., Bhattacharya S., Sur S., Sengupta C., Das S.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
|
2021
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tom 102
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nr 3
2

The complete structure of the cucumber [Cucumis sativus L.] chloroplast genome: its composition and comparative analysis

86%
Plader W., Yukawa Y., Sugiura M., Malepszy S.
Cellular and Molecular Biology Letters
|
2007
|
tom 12
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nr 4
584-594
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp, which are separated by two single-copy regions, a small 18,222-bp one and a large 86,688-bp one. The chloroplast genome of cucumber contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes (4 rRNA species), and 37 tRNA genes (30 tRNA species), with 18 of them located in the inverted repeat region. Of these genes, 16 contain one intron, and two genes and one ycf contain 2 introns. Twenty-one small inversions that form stem-loop structures, ranging from 18 to 49 bp, have been identified. Eight of them show similarity to those of other species, while eight seem to be cucumber specific. Detailed comparisons of ycf2 and ycf15, and the overall structure to other chloroplast genomes were performed.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA

86%
Anand A., Chauhan S., Chodon A., Kumar K. V. V., Saravanakumar S., Pandi G.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2024
|
tom 105
|
nr 1
4

New DNA markers for discrimination between closely-related species and for the reconstruction of historical events; an example using liverworts

72%
Szweykowska-Kulinska Z., Pacak A., Jankowiak K.
Cellular and Molecular Biology Letters
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2002
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tom 07
|
nr 2A
A survey of fully-sequenced chloroplast genomes revealed that in land plants there are six tRNA genes that have introns. Moreover, the length of a particular tRNA gene intron remains relatively stable across species. However, in algae, the presence of chloroplast tRNA genes containing introns is exceptional. A survey of mitochondrial plant genomes revealed intron-containing tRNA genes are rather rare features, with the exception of tRNASerGCU genes in liverworts and peat-mosses. We isolated and sequenced one mitochondrial and three chloroplast intron-containing tRNA genes and a fragment of the mitochondrial coxIII gene containing the first intron from the following liverwort species: Pellia borealis, Pellia epiphylla-species N, Pellia epiphylla-species S and Porella baueri, Porella cordaeana, Porella platyphylla. We showed that, as in the case of higher plants, the rate of nucleotide substitution is lower in the mitochondrial genome than in the chloroplast genome. Moreover, the comparison of intron nucleotide sequences enabled us to show that in the case of one allopolyploid species, Pellia borealis, organelles were transmitted from one parent species, Pellia epiphylla-species N. In the case of another allopolyploid species, Porella baueri, organelles were also inherited from one parent species, Porella cordaeana. Therefore, organellar inheritance in liverworts seems to be uniparental. It remains clear that analysis of carefully chosen chloroplast and mitochondrial DNA sequences allowed us to reconstruct historical events.
5

Cytogenetic analysis of diploid Brassica species

58%
Hasterok R., Maluszynska J.
Acta Biologica Cracoviensia. Series Botanica
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2000
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tom 42
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nr 1
The chromosomes of Brassica species are small and poorly differentiated, and their identification is extremely difficult using conventional cytogenetic methods. Progress in molecular analysis of Brassica species requires cytogenetic maps of their chromosomes. Chromosome-specific markers are needed to distinguish particular pairs of homologous chromosomes and for karyotyping. In this study, three morphological groups of chromosomes in B. campestris (genome A) and B. oleracea (genome C) and two in B. nigra (genome B) were distinguished by the morphometric features of the chromosomes, based upon arm ratio and absolute length. Using fluorescence in situ hybridization and differential stainings it was possible to establish further markers for five pairs of chromosomes in genome A, three in genome B and two in genome C, and to present idiograms of the chromosomes for three diploid Brassica species. However, for clear identification of all chromosome pairs more cytogenetic markers are needed.
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