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Three accessions of winter savory (S. montana) and two other perennials from Satureja genus (S. amoena and S. coeuela) were compared in respect of the content and composition of essential oil, and tannin content as well. The content of essential oil ranged from 0.70% to 1.90%. The main component of essential oil in all accessions was carvacrol. In two populations (5. amoena from Poland and S. montana from Canada) it constituted more than 70%, in three other about 40%. The important constituents were also: γ-terpinene and p-cymene. The content of γ-terpinene ranged from 6.49 to 17.28% and p-cymene from 5.58 to 29.98%. S. coeuela from Austria was characterized by high concentration of terpinene-4-ol (13.92%). The content of tannins in herb ranged from 2.31 (S. montana from Canada) to 4.01% (S. coeuela from Austria).
Common oregano is an important medicinal and aromatic plant, characterized by a high morphological and chemical variability. The aim of the work was to determine the diversity of 14 common oregano populations introduced into ex situ conditions, in respect of morphological and developmental traits as well as content and composition of essential oil in herb. Observations and harvest of raw material were performed in the second year of plant vegetation. Morphological and developmental characters were estimated. Total content of essential oil was determined according to European Pharmacopoeia 8th. The composition of essential oil was carried out by gas chromatography. Populations differed in respect of examined traits, i.a.: plant’s height from 52.13 to 88.66 cm, number of stems per plant from 45.6 to 123.3, number of internodes per stem from 4.6 to 9.2. Fresh mass of herb was at a level of 249.0–896.6 g per plant. Total content of essential oil ranged from 0.35 to 0.87%. Sabinene, 1,8-cineole, linalool, p-cymene, β-caryophyllene and caryophyllene oxide were the dominant compounds in essential oil. Number of stems and fresh mass of herb differentiated examined populations at the highest degree. Most of populations were classified as a sabinyl chemotype rich in sesquiterpenes.
The aim of the study was an ex situ evaluation of morphological and chemical variability within the population of common avens. Plant height, number of shoots, stage of generative development (number of flower buds, flowers, and fruits), as well as weight of herb and underground organs were determined. In the raw materials total content of tannins and content of particular phenolic compounds identified by HPLC was determined. In underground organs also content of eugenol – the dominant constituent of essential oil was assessed. High diversity of morphological traits (especially weight of herb and underground organs) and the content of determined compounds (especially in herb) was found. Above- and underground organs differed in the composition and content of phenolic compounds. Underground organs were characterised by higher content of flavan-3-ols, but they did not contain chlorogenic acid, which was the dominant phenolic compound in herb.
Eight accessions of St. John's wort (Hypericum perforatum L.) originating from natural sites in Lithuania were investigated ex situ. The content and composition of flavonoids and phenolic acids, as well as the contents of hypericin and pseudohypericin in the herb were determined. The total contents of flavonoids and phenolic acids ranged from 2.42% to 3.10% and from 1.16% to 3.17%, respectively. The dominant flavonoid compounds were rutin, hyperoside and isorhamnetin-3-rutinoside. The presence of two phenolic acids (chlo- rogenic and 4-hydroxyphenylacetic one) was also stated. The content of hypericin and pseudohypericin ranged from 37.01 to 99.12 mg/100g.
The study determined genetic and chemical variability of 40 populations of E. angustifolium and E. hirsutum, growing wild in the eastern Poland. The RAPD analysis indicated higher genetic variability in E. angustifolium than in E. hirsutum. The contents of biologically active phenolic compounds (flavonoids, phenolic acids, tannins) in dry herb were determined. Qualitative analysis of flavonoids was performed by HPLC. There were differences in the content of flavonoids, phenolic acids and tannins in the populations of both species. The main flavonoid compounds were - luteolin-7-glucoside in E. angustifolium herb and rutin in E. hirsutum herb.
The experiment was conducted in 2003-2004. The aim of the research was to investigate the chemical variability of three thyme species (Thymus pulegioides L., Thymus alpestris Tausch., Thymus kotschyanus Boiss. & Hohen) under „in situ” conditions. Six localities were selected and fixed by GPS. Samples of raw materials from the stands were collected, dried and chemically analysed. The content of essential oil and its chemical composition as well the content of flavonoids, tannins, polyphenolic acids and bitters were determined. The obtained results indicated significantly chemical differences between the investigated populations of Thymus species. The content of essential oil in the dry raw material ranged from 0.46 ml·100 g⁻¹ DM to 0.93 ml·100 g⁻¹ DM. The content of tannins was similar for T. pulegioides (5.44% on the average). However the content of tannins for T. alpestris ranged from 2.56% to 7.12%. The highest content of polyphenolic acids was determined in the herb of T. pulegioides (2.61%) from „Jaworzec”.
The chemical composition of the herb of four populations of common burstwort (Herniaria glabra L.) growing wild in the middle part of the Bug river valley was compared. The investigated populations differed significantly as to the content of saponins (3.47-5.29 g/100 g), tannins (0.75- 1.40 g/100 g) and flavonoids (620.7-1071.9 mg/100 g), but only slightly in the content of polyphenolic acids (0.64-0.84 g/100 g). The predominant phenolic acids turned out to be pyrocatechuic and p- hydroxybenzoic acids, and the main flavonoid compounds were isorhamnetin 3-rhamnoglucoside and rutin.
The inflorescences of yellow everlasting collected from twenty two natural sites in the middle part of the Bug river valley and two in the Puszcza Knyszyńska forest were compared in respect to the content and composition of flavonoids and phenolic acids. Genetic distances among populations, evaluated by RAPD markers, fluctuated from 0.0968 to 0.4855. There were no dependences between genetic relationships and geographical distributions of populations, as well as between their genetic relationships and chemical compositions. The total content of flavonoids ranged from 0.15 to 0.70% and phenolic acids from 0.45 to 1.52%. As a result of the HPLC analysis no flavonoid compounds were identified, with isosalipurposide as a dominant constituent. The chlorogenic, caffeic, ferulic and rosmarinic acids were also detected.
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