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Fe[II], Cu[II] and Zn[II] chelating activity of buckwheat and buckwheat groats tannin fractions

100%
Karamac M.
Polish Journal of Food and Nutrition Sciences
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2007
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tom 57
|
nr 3
357-362
Phenolic compounds were extracted from buckwheat and buckwheat groats into 80% acetone (v/v). The crude extracts were separated with Sephadex LH-20 column chromatography into low molecular weight phenolic compounds and tannin fractions. The tannin fractions were characterised by colour reaction with vanillin/HCl reagent, ability for protein precipitation as well as SE-HPLC method. Chelation of Cu(II) and Zn(II) by tannin fractions was determined by reaction with tetramethylmurexide (TMM) whereas Fe(II) chelation was investigated by forming complexes with ferrozine. The tannin fractions obtained from buckwheat and buckwheat groats gave similar results in vanillin test; A500/mg was 1.04. Also results obtained using precipitation method were comparable. SE-HPLC chromatograms showed that in tannin fraction of buckwheat groats polymers with molecular weight ≥ tannic acid were present. On the chromatogram of buckwheat tannins peaks were shifted to lower molecular weight compounds. Tannins of buckwheat were stronger chelators of all metal ions tested (Fe(II), Cu(II) and Zn(II)) than tannins of buckwheat groats. Ions of copper were chelated the most effectively; 1 mg of buckwheat and buckwheat groats tannin fractions added per assay bound 86.0% and 72.9% of Cu(II), respectively. Buckwheat tannins used in the test at a level of 2.5 mg/assay bound approximately twice more Fe(II) than the same addition of buckwheat groats tannins. Zn(II) was chelated to the lowest extent, 17.2% was bound by buckwheat tannins and 13.5% by buckwheat groats tannins (5 mg of tannin/assay).
2
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Stress-induced behaviour in adult and old rats: effects of neonatal asphyxia, body temperature and chelation of iron

80%
Rogalska J., Caputa M., Wentowska K., Nowakowska A.
Journal of Physiology and Pharmacology. Supplement
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2006
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tom 57
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nr 8
17-34
Perinatal asphyxia in mammals leads to iron accumulation in the brain, which results in delayed neurobehavioural disturbances, including impaired learning and abnormal alertness over their entire life span. The aim of this investigation was to verify our hypothesis that newborn rats, showing reduced normal body temperature, are protected against neurotoxicity of the asphyxia up to senescence. Alertness was studied in adult and old male Wistar rats after exposure to critical neonatal anoxia: (i) at physiological neonatal body temperature of 33°C, (ii) at body temperature elevated to 37°C, or (iii) at body temperature elevated to 39°C (the thermal conditions remained unchanged both during anoxia and for 2 h postanoxia). To elucidate the effect of iron-dependent postanoxic oxidative damage to the brain, half of the group (iii) was injected with deferoxamine, a chelator of iron. Postanoxic behavioural disturbances were recorded in open-field, elevated plus-maze, and sudden silence tests when the rats reached the age of 12 and 24 months. Open-field stress-induced motor activity was reduced in rats subjected to neonatal anoxia under hyperthermic conditions. In contrast, these rats were hyperactive in the plus-maze test. Both the plus-maze and sudden silence tests show reduced alertness of these rats to external stimuli signalling potential dangers. The behavioural disturbances were prevented by body temperature of 33°C and by administration of deferoxamine.
3

Aktywnosc przeciwutleniajaca wysokotaninowych ekstraktow z surowcow roslinnych

51%
Amarowicz R., Troszynska A., Karamac M.
Bromatologia i Chemia Toksykologiczna
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2005
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tom 38
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nr Supl.
193-195
Z nasion fasoli adzuki, gryki, kaszy gryczanej orzechów włoskich i laskowych oraz migdałów otrzymano ekstrakty związków fenolowych stosując 80% aceton. Zawartość fenoli ogółem i tanin w ekstraktach oznaczono metodami kolorymetrycznymi. Ekstrakty wykazywały zróżnicowaną aktywność przeciwutleniającą i antyrodnikową oraz różną zdolność do chelatowania Fe(II).
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