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Charophytes possess the capacity to store large amounts of various elements, but until today no studies about known methods of determining CaCO₃ and elements in charophytes have been conducted. Because of the large and varying amounts of CaCO₃, it is difficult to estimate the actual element content in dry mass. Simple comparisons of elements in charophyte plant tissue or between charophytes and higher plants are impossible. The main aim of this study was to investigate through laboratory experiments content of carbonates and elements in charophyte tissue using different methods of decomposition (mineralization). The proposed protocol: determination of calcium carbonate content in plants, determination of elements present in the plant dry mass, and calculation of the element content in the plant dry mass as described.
Interdisciplinary investigations of 39 lakes of the Lubuskie Lakeland (mid-Western Poland) were undertaken in the vegetation seasons 2002–2009. The aim of presented study was to characterize the significance of charophytes as indicators of habitat differentiation of lakes using the number of species, number of communities and the percentage share in the phytolittoral area. Each lake was characterized based on the results of macrophyte vegetation, vegetation depth extent, pelagic phytoplankton biomass, lakes area, max. depth, SD visibility, pH, conductivity, O₂, total hardness, Ca, Mg, PO₄, TP, NH₄, NO₃, NO₂, N org., TN. The statistical analyses (PCA), revealed that the investigated lakes were differentiated according to the basic morphometrical and physicochemical parameters and that the differentiation was reflected in the percentage share of charophytes in phytolittoral (in general – the bigger, deeper and more clear water were the lakes, the greater part of phytolittoral was covered by charophytes). Using simple cluster analysis based on the number of different types of macrophyte communities (helophytes, elodeids, nymphaeids and charophytes) two distinct groups of lakes were distinguished. Charophyte communities were at most responsible for obtained differentiation (one group with 0 or 1 and second with 1–6 charophyte communities). The distinguished groups of lakes differed significantly as regards 12, out of all 18 considered parameters. The greatest differences were evidenced for area, max. depth, SD visibility, Ca, Mg, NH₄, NO₃, phytoplankton biomass and vegetation depth extent. Similar analyses were made for the number of charophyte species, for which the lakes were divided into four groups (0– 1, 2–3, 4–5 and 6–7 charophyte species per lake). In this case 11 parameters significantly differed between distinguished groups of lakes, showing, that better habitat conditions were in the lakes where more charophyte species were stated. The obtained results revealed that all analyzed charophyte indicators (the number of species, number of communities as well as the percentage share in the phytolittoral area) very clearly reflect habitat variation and water quality of lakes and thus may be used as valuable bioindicators of lakes condition.
Primary producers are able to strongly affect calcium budget in hardwater lakes. The relative contribution of phytoplankton and charophytes to water decalcification (by precipitation of calcium carbonate) is, however, unclear. In this study we checked the effect of natural phytoplankton community and a charophyte (Nitellopsis obtusa) on the decline of calcium concentration in experimental outdoor conditions. The experiment was carried out in original lake water and two variants of enrichment with inorganic nitrogen and phosphorus to test the changing efficiency in decalcification by both primary producers. At low nutrient concentrations, N. obtusa was responsible for calcium decline in original lake water by 12 mg Ca+2 dm–3 during 20 days of experiment. In these conditions the effect of phytoplankton was negligible. In lake water enriched with nutrients, the exponential growth of phytoplankton led to the decrease of calcium concentration from initial 35 mg Ca+2 dm–3 to 9 mg Ca+2 dm–3 in the same time period. The maximum effect of N. obtusa was the same as in original lake water but manifested itself earlier to decline in the end of experiment. Supersaturation of water with calcium carbonate was always more than threefold and saturation index reached 27 in mixed cultures of phytoplankton and N. obtusa in lake water enriched with nutrients. In this context we hypothesise on a possible role of charophytes as nucleation sites necessary for calcite precipitation. Based on our own and literature data we also discuss expected immobilisation of phosphate incorporated in calcite precipitated by the growth of phytoplankton and N. obtusa.
Regarding their distribution in lakes, Chara delicatula and Chara globularis are considered species of different or even opposite ecological requirements. C. delicatula is usually reported from oligotrophic lakes, but C. globularis from more fertile ones. Within Poland, both species rarely build extensive stands in the same ecosystem. The present study was carried out in a stratified, mid-forest lake in midwestern Poland where C. delicatula had not been found earlier. Based on the physical-chemical properties and analyses of phytoplankton, a transitional, meso-eutrophic status of the lake was stated. In the group of 15 more frequent macrophyte species, C. delicatula and C. globularis were among plants predominating the lake’s macrovegetation. Both stoneworts built separate patches as well as contributed to other macrophyte assemblages. Growing under the same light and trophic conditions, the species revealed differences in ecological optima in relation to the depth of occurrence and bottom slope. The results are discussed in the context of the species identity: are C. delicatula and C. globularis separate species or forms within the same taxon?
Microorganisms associated with aquatic macrophytes can in various ways interact with a plant and influence its activity and vice versa. A low-salinity intrusion into freshwater environment can affect plant-microorganism interactions. In this study, effects of different salinity conditions on the abundance and community composition of associated microorganisms with charophytes in the Curonian Lagoon were assessed. From the results, we found that short term salinity changes affected the abundance of bacteria and fungi associated with charophytes, whereas no response was reflected in the taxa composition of fungi, showing that other factors could be of more importance. The increased fungi abundances and different fungi composition in August in comparison to June was probably related to senescence process of aquatic vegetation. 8 fungi taxa were isolated and identified in association with charophytes, while higher diversity was revealed by DGGE technique.
At present Lychnothamnus barbatus (Meyen) Leonhardi belongs to the rarest species of charophytes in the world. In Europe it is classified as threatened with extinction. The problem of extinction of this species is intriguing, in particular in the context of its widespread occurrence in Europe and Asia till the last decade of the 20th century. Records of L. barbatus from Wielkopolska region (Western Poland) are know from 15 lakes. The most of them was stated in 19th and on beginning of 20th centuries. Now, this species is growing in 6 lakes, from among 2 sites are new. This study was undertaken to a) determine the abundance of L. barbatus and the co-occurring plant species at different sites in lakes, b) determine the most important ecological parameters controlling the structure communities with L. barbatus co-occurring and quantitative responses of this species. In 7 lakes (area 5.5-197 ha, depth max. 7.8– 38 m, trophic state: meso-eutrophic) in western Poland the species composition and coverage of vegetation were studied at the 23 plots with L. barbatus occurrence in relation to the measured variables. Seventeen environmental parameters were measured including: depth of water, pH, conductivity, SO₄²⁻, NH₄⁺, NO₃⁻, PO₄³⁻, Na⁺, K⁺, Ca²⁺, Mg²⁺, chlorophyll a, Secchi disc visibility, colour, O₂ dissolved, saturation, total Fe during the period July–September. The DCA and CCA analyses were used to assess the relation between vegetation parameters and environmental variables. L. barbatus preferred the water rich in Ca⁺, Mg²⁺ and SO₄²⁻ and with high concentrations of nutrients, especially NH₄⁺ and PO₄³⁻, and moderate values of electrolytic conductivity. In the studied lakes, L. barbatus occupied the separate niche. This species formed the communities in very shallow marginal zones of lakes (0.4–1.5 m) with other macrophytes like: Chara vulgaris, C. tomentosa, Potamogeton ´ nitens, and monospecific stands on margins of steep lake slopes (4–6 m) with Chara globularis fo. hedwigii and Nitella mucronata. The process of extinction of this species seems to be related with increasing turbidity related in turn to algal blooming in lakes and with the spatial competition of vascular macrophytes, especially Ceratophyllum demersum.
Morphological features of oospores of Chara baueri A. Braun, one of the rarest charophyte species worldwide, were studied based on 100 oospores collected from a small and temporarily dried mid-field pond near Cedynia, Western Poland. This is the first Polish and fifth presently known locality of this species. For comparison 67 oospores from a German population (similar pond localized near Batzlow, Germany) were also measured. So far, data on morphology of C. baueri oospores as well as the species ecology are limited. The only more detailed study of oospores for this species was earlier performed on 15 oospores from Kazakhstan. Largest polar axis (LPA, length), largest equatorial diameter (LED, width), isopolarity index (ISI = LPA/LED × 100), number of ridges, width of fossa, distance from apical pole to LED (AND) and anisipolarity index (ANI = AND/LPA × 100) were measured. The comparative analysis revealed that the oospores from Poland are generally bigger and more prolate than the German ones. The differences for most of studied parameters were statistically significant. The finding is discussed in the context of habitat differentiation of both studied sites. Moreover, the results obtained of oospore measurements for both populations differs from most of the data known so far from the literature.
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The distribution and ecology of charophytes in Lubelszczyzna (Eastern Poland) is described based on herbaria collections, literature data, and the author’s own investigations. Maps showing the distribution of all 22 charophyte species identified so far are presented, as well as the details of habitats ecology. Some data on species frequency, conservation, and threats are added.
This paper presents recent localities of charophytes in the peatland complex of the northern part of Noteć Forest (Wielkopolska, Poland). In alkaline or calcareous fens and shallow waters, seven species of charophytes were found: Chara globularis, C. intermedia, C. virgata, C. vulgaris, Nitella capillaris, N. mucronata and N. syncarpa. Some of the charophytes, which were observed in this area, are regarded as endangered and vulnerable in Poland, especially, C. intermedia, N. capillaris and N. syncarpa. The preliminary research results on the studied charophyte distribution and ecology are presented.
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