Wyniki wyszukiwania

1

Purification and some properties of exo-1,4-beta-glucanase from Chaetomium olivaceum

100%

El-Gindy A. A., Saad R. R., Fawzi E.

Acta Microbiologica Polonica

|

2003

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tom 52

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nr 1

2

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Screening of cellulase and pectinase by using Pseudomonas fluorescens and Bacillus subtilis

88%

Reetha S., Selvakumar G., Bhuvaneswari G., Thamizhiniyan P., Ravimycin T.

International Letters of Natural Sciences

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2014

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tom 08

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nr 2

A study was conducted to determine the Production of cellulase and pectinase enzyme by using Plant growth promoting rhizobacteria like Pseudomonas fluorescence and Bacillus subtilis. These to micro organism are isolated by serial dilution method. One gram of soil sample was diluted in to 10 ml of sterile distilled water and 1 ml of sample solution was serially diluted in 9ml of sterile water up to 10 dilution. Each sample from dilution 10-5 and 10-6 were taken and streaked in to KB and NA medium and incubate at 24 hrs. After 24 hrs Pseudomonas fluorescence and Bacillus subtilis was observed in the medium of KB and NA medium. Both the culture was sub cultured and maintain in the same for the further work. CMCase medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria and incubates for 48h after incubation period a clear halo zone was produced by these bacteria among these bacteria Pseudomonas fluorescence are able to produce high amount of cellulose compare to Bacillus subtilis. Pectin agar medium was prepared and sterilized by autoclave for 121 ºC for 15 minutes after sterilization these medium contain petriplate was streaked by bacteria incubates for 48h after incubation period a clear halo zone was produced by these bacteria, among these bacteria Pseudomonas fluorescence are able to produce high amount of Pectinase compare to Bacillus subtilis. Plant growth promoting rhizobacteria (PGPR) are beneficial bacteria that colonize plant roots and enhance plant growth by a wide variety of mechanisms.

3

Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials

75%

Rajasekharan S. K., Ramesh S.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

Burkholderia cepacia is an opportunistic pathogen causing infections in patients with cystic fibrosis. Patients with implanted devices are prone to B. cepacia infections due to its ability to grow as biofilms. Knowing the importance of polysaccharides in a biofilm, enzymes that degrade them were targeted as a possible candidate for antibiofilm agents. In this study, the antibiofilm potential of cellulase against B. cepacia biofilms formed on various prosthetic materials was tested. Cellulase exhibited significant antibiofilm activity against B. cepacia without having much action on its growth, thus ruling out the chance of selection pressure and subsequent development resistance.

4

Immunocytochemical localisation of cellulases in tomoto roots infected with potato cyst nematodes

75%

Kurek W., Lichocka M., Golinowski W.

Polish Journal of Natural Sciences. Supplement

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2003

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tom 01

5

New inducers for cellulases production by Trichoderma reesei M-7

75%

Janas P., Targonski Z., Mleko S.

Electronic Journal of Polish Agricultural Universities. Series Food Science and Technology

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2002

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tom 05

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nr 1

The aim of work was to examine the influence of lactobionic acid, gluconic acid, delta-gluconolactone, lactulose and sorbitol on the production of cellulases by mutant strain Trichoderma reesei M-7 during batch and continuous cultivation. Positive results were achieved in the presence of lactobionic acid and lactulose as inducers. In both cases the highest inductive effect of cellulase production was observed in the presence of 1% mixtures of above mentioned substrates at the ratio 1:1 (0.5% : 0.5%) with lactose. (about 20% higher than that observed in the presence of 1% of lactose) The induction mechanism of lactobionic acid and lactulose may be attributed by slow rate of their utilisation (slower than lactose). Explanation of the inductive mechanism of lactose, lactobionic acid and lactulose on cellulases production by Trichoderma reesei demands further investigations among other with using a purified beta-galactosidase enzyme.

6

Expression of cellulases and expansins during induction and maintainance of syncytia induced by potato cyst nematode [Globodera rostochiensis] in roots of susceptible tomato

75%

Sobczak M., Fudali S., Janakowski S., Karczmarek A., Golinowski W.

Polish Journal of Natural Sciences. Supplement

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2003

|

tom 01

7

Effect of cellulase and xylanase on in vitro TMR digestibility and the performance of dairy cows

63%

Wylegala S., Nowak W., Kruczynska H.

Polish Journal of Natural Sciences. Supplement

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2006

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tom 03

265-269

8

Production of extracellular enzymes by Trichoderma reesei M-7 on mixtures of lactose and glucose at different temperatures

63%

Janas P., Targonski Z., Udeh K. O., Wasko A.

Polish Journal of Food and Nutrition Sciences

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2002

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tom 11/52

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nr 1

Continuous cultivation of Trichodcnna reesei M-7 mutant was performed at different temperatures (26, 30 and 34°C) with 1% lactose or glucose alone or a total of 1% mixtures of both sugars at different ratio. The secretion of individual enzymes was affected by the ratio of cellulase inducer (lactose) to the repressor (glucose). The ratio of enzyme secretions was additionally modified by the temperature of cultivations. An insignificant increase of nonspecific activity of cellulases (FPU) and significant increase of aryl-ß-glucosidase activity were observed at 26°C with lactose/glucose ratio of 3:1 and 1:1. However, when cultivated both at 30 or 34°C, the cellulolytic activities (FPU) increased significantly in the presence of 1% glucose alone. The activity of xylanases increased significantly (about 8-fold) with lactose/glucose ratio of 1:1 at 30°C. The increased synthesis of lytic enzymes (proteases, ß-1,3-glucanases) correlated with increased glucose concentration in the feeding medium and this effect potentiated at 34°C of cultivation.

9

Production of extracellular enzymes by low-protease mutants of Trichoderma reesei

63%

Janas P.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2003

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tom 02

|

nr 2

Cultivation has been performed of isolated earlier low-protease mutants and parental strain in the presence of novel inducers of cellulases production - lactulose and lactobionic acid mixed with lactose in the ratio 1:1. Nine among ten tested during batch cultivation low-protease mutants exhibited lower ability for the production of proteases than parental strain M-7. Higher enzymatic activities have been estimated (cellulolytic, xylanolytic and beta - galaktosidase) of culture filtrates during cultivation in the presence of mixtures of lactobionic acid and lactose in comparison to lactulose and lactose. Tested, during continuous cultivation, low-protease mutant T. reesei Mp5 exhibited high resistance for the temperature shifting. FPU activities of culture filtrates were stable in the high range of temperature of cultivation (26-34°C). In addition current knowledge about correlation between protease and other enzymes of culture fluids of selected filamentous fungi has been reviewed. The ways of preventing homologous and heterologous protein produced by fungi and bacteria from enzymatic cleavage by proteases have been also described. This work is an introduction for future studies on correlation between proteases and other enzymes in culture filtrates of Trichoderma reesei.

10

Production of cellulases during citric acid biosynthesis by solid-state fermentation

63%

Gasiorek E., Lesniak W.

Polish Journal of Food and Nutrition Sciences

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2002

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tom 11/52

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nr 1

The studies on optimisation of citric acid biosynthesis by solid-state fermentation of sugar-beet pulp as the major material have shown that not only mono- and disaccharides initially present in the material but also the products of cellulose and hemicellulose hydrolyses were the carbon sources. Therefore, in parallel with optimisation of citric acid production, the studies were undertaken on the activity of ccllulases and dynamics of their synthesis by Aspergillus niger, the strain used in the process. The fermentations were carried out by non-mixed system using 1 L beakers and by mixed system using 4.5 L rotating drum bioreactor. The activity of CMC-ase and xylanases for the non-mixed system increased to about 22.0 IU/g and that of FP-ase stayed on the level of about 4 IU/g dry matter, whereas for the mixed system the CMC-ase activity increased to about 35 IU/g dry matter on day five and that of FP-ase stayed on a similar level of about 5 IU/g dry matter. The content of reducing compounds increased by almost four times (to 390 g/kg dry matter) on the second day of fermentation and decreased to about 140 g/kg dry matter on the fifth day of fermentation. Glucose was utilised most rapidly among monosaccharides, being followed by arabinose and in part by mannose.

11

Cellulolytic and pectolytic activity of bacilli isolated from the root-free soil and the rhizosphere of different forest trees

63%

Gierasimiuk J., Strzelczyk E.

Folia Forestalia Polonica. Series A . Forestry

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2003

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tom 45

12

Geotrichum hydrolytic activity in milled malt and barley medium

51%

Piegza M., Witkowska D., Stempniewicz R., Rywinska A.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2005

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tom 08

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nr 1

13

Biosynteza fitaz, fosfataz oraz celulaz i ksylanaz w hodowlach grzybów strzępkowych w podłożu stałym

51%

Rywinska A., Witkowska D., Piegza M., Jarosz M., Salamon J.

Acta Scientiarum Polonorum. Biotechnologia

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2007

|

tom 06

|

nr 2

14

Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris

51%

Golinska P., Dahm H.

Dendrobiology

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2011

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tom 65

The enzymatic activity (chitinolytic, proteolytic, pectolytic andcellulolytic) of twenty strains of Streptomyces isolated from soil and rhizosphere of Scots pine was analyzed. Most strains produced chitinases, catalyzing the degradation of chitin, the main component of fungal cell walls including fungi pathogenic for plants. This activity was about 4 times higher in the presence of Fusariumoxysporum than Rhizoctonia solani mycelium or chitin flakes. The number of the proteolytic strains was also significant. In general, rhizosphere and soil organisms synthesizedcomparable amounts of these enzymes. Over half of the analyzed Streptomyces strains produced pectolytic enzymes (polygalacturonase, pectin lyase and pectate lyase). This property was more common among rhizosphere than among soil strains. The Streptomyces strains also showedcellulolytic activity (endocellulases, exocellulases) – enzymes decomposing basic components of cell walls of plant and lower fungi (cellulose). The cellulolytic activity was differentiated and depended on the Streptomyces strain. Conclusion of our studies is that Streptomyces are the microorganisms more chitinolytic andproteolytic than pectolytic and cellulolytic.

15

Immunocytochemiczna lokalizacja celulazy w korzeniach rzepaku porazonych matwikiem burakowym [Heterodera schachtii]

51%

Gorecka M., Kurek W., Golinowski W.

Rośliny Oleiste - Oilseed Crops

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2003

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tom 24

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nr 2

489-500

16

Capability of Geotrichum candidum yeasts for cellulases and xylanases biosynthesis

51%

Witkowska D., Piegza M.

Electronic Journal of Polish Agricultural Universities. Series: Biotechnology

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2006

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tom 09

|

nr 4

17

Wpływ enzymów wspomagających na skład chemiczny i lepkość zacierów z pszenżyta

51%

Sapinska E., Balcerek M.

Acta Agrophysica

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2013

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tom 20

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nr 1

W pracy oceniano wpływ dodatku preparatów enzymów wspomagających (ksylanazy, pullulanazy, celulazy i celobiazy) do zacierów z pszenżyta, przygotowanych metodą bezciśnieniowego uwalniania skrobi (BUS) na ich skład fizyko-chemiczny, lepkość oraz wskaźniki fermentacji. Fermentację prowadzono systemem trzydobowym w temperaturze 28-30°C, z udziałem suszonych drożdży gorzelniczych Saccharomyces cerevisiae, rasy As4. Na podstawie otrzymanych wyników stwierdzono wpływ dodatku enzymów wspomagających na własności reologiczne i skład fizyko-chemiczny, zarówno zacierów słodkich (ekstrakt, cukry redukujące, dekstryny, lepkość), jak i odfermentowanych (ekstrakt pozorny, ekstrakt rzeczywisty, alkohol, cukry redukujące, dekstryny) oraz przebieg fermentacji. W próbach fermentacyjnych, zawierających ksylanazę, pullulanazę oraz celulazę i celobiazę zaobserwowano poprawę dynamiki procesu fermentacji – szybsze zafermentowanie i krótszy czas trwania procesu w porównaniu z próbą kontrolną (bez dodatku enzymów wspomagających). W zależności od rodzaju zastosowanych enzymów pomocniczych, wydajność fermentacji mieściła się w granicach od 88,10% (zacier z ksylanazą) do 94,92% wydajności teoretycznej (zacier z celulazą i celobiazą) i była znacząco wyższa niż w próbie kontrolnej (65,60%).

18

Polysaccharase activities of Chaetomium murorum and C.bostrychodes grown on groundnut shells

51%

El-Gindy A. A., Saad R. R.

Acta Microbiologica Polonica

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1992

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tom 41

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nr 1-2

19

Enzymatic activities of Aspergillus fumigatus strains isolated from the air at waste landfills

51%

Krikstaponis A., Lugauskas A., Krysinska-Traczyk E., Prazmo Z., Dutkiewicz J.

Annals of Agricultural and Environmental Medicine

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2001

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tom 08

|

nr 2

20

Effect of pH on biosynthesis of cellulase, xylanase and lytic enzymes by Trichoderma reesei M-7 in fed-batch culture

51%

Targonski Z., Pielecki J.

Acta Microbiologica Polonica

|

1993

|

tom 42

|

nr 3-4

Ograniczanie wyników

Purification and some properties of exo-1,4-beta-glucanase from Chaetomium olivaceum

Screening of cellulase and pectinase by using Pseudomonas fluorescens and Bacillus subtilis

Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials

Immunocytochemical localisation of cellulases in tomoto roots infected with potato cyst nematodes

New inducers for cellulases production by Trichoderma reesei M-7

Expression of cellulases and expansins during induction and maintainance of syncytia induced by potato cyst nematode [Globodera rostochiensis] in roots of susceptible tomato

Effect of cellulase and xylanase on in vitro TMR digestibility and the performance of dairy cows

Production of extracellular enzymes by Trichoderma reesei M-7 on mixtures of lactose and glucose at different temperatures

Production of extracellular enzymes by low-protease mutants of Trichoderma reesei

Production of cellulases during citric acid biosynthesis by solid-state fermentation

Cellulolytic and pectolytic activity of bacilli isolated from the root-free soil and the rhizosphere of different forest trees

Geotrichum hydrolytic activity in milled malt and barley medium

Biosynteza fitaz, fosfataz oraz celulaz i ksylanaz w hodowlach grzybów strzępkowych w podłożu stałym

Enzymatic activity of actinomycetes from the genus Streptomyces isolated from the bulk soil and rhizosphere of the Pinus sylvestris

Immunocytochemiczna lokalizacja celulazy w korzeniach rzepaku porazonych matwikiem burakowym [Heterodera schachtii]

Capability of Geotrichum candidum yeasts for cellulases and xylanases biosynthesis

Wpływ enzymów wspomagających na skład chemiczny i lepkość zacierów z pszenżyta

Polysaccharase activities of Chaetomium murorum and C.bostrychodes grown on groundnut shells

Enzymatic activities of Aspergillus fumigatus strains isolated from the air at waste landfills

Effect of pH on biosynthesis of cellulase, xylanase and lytic enzymes by Trichoderma reesei M-7 in fed-batch culture