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Immunospecific nuclear proteins of colorectal tumors

100%

Szymczyk P., Krajewska W. M., Jakubik J., Berner A., Berner J., Brocki M., Rajczyk B., Kilianska Z.

Cellular and Molecular Biology Letters

1996

tom 01

nr 4

Electrophoretically specific nuclear proteins of human colon adenocarcinoma with mol. wt of 35-40 kDa were used as immunogen to produce rabbit antiserum. Expression of cancer-specific antigens was investigated by Western blot technique among nuclear proteins from normal and cancerous mucosa. Obtained antiserum crossreacted mainly with 36 kDa polypeptide in 23 of 26 (88,5%) colorectal tumor nuclear fractions but not with any of normal ones. It was also observed that this antiserum recognized 36 kDa antigen in 10 of 12 and 6 of 7 nuclear fractions from other cancers, ie. gastric and lung, respectively. In part of studied tumors antiserum crossreacted also with the antigens of 38 and 32-33 kDa. Expression of 36 and 32-33 kDa components seems to be correlated with colorectal cancer progression from A to B stage of disease according to the classification of Dukes. Immunoblot analysis revealed that cancer-specific 36 kDa polypeptide, mainly associated with nuclear compartment, can be also detected within 10P and 100P fractions of colorectal tumors.

The protective role of astroglia in the early period of experimental lead toxicity in the rat

67%

Struzynska L.

Acta Neurobiologiae Experimentalis

2000

tom 60

nr 2

Although the clinical manifestations of lead (Pb) neurotoxicity are documented, the subcellular mechanisms of its action are still an open question. The purpose of this study was to assess the function of nerve ending particles after acute lead exposure and to investigate whether it exerts a toxic effect on astroglial functions. The studies were performed using the rodent model of acute lead toxicity. Cellular fractions were used in biochemical measurements - synaptosomes and glial plasmalemmal vesicles (GPV). Since a procedure for the isolation of the fraction of astroglial origin has been developed, it becomes possible to investigate lead-astroglia interactions after in vivo exposure. It is of importance because most of the studies concerning lead toxicity were performed using astroglial culture systems. It was found that the uptake of glutamate (Glu) to the synaptosomes was lowered and KCl-dependent release was increased, suggesting the impairment of glutamatergic transmission leading to the elevation of extracellular amino acid concentration. In contrast, glutamate uptake to the GPV fraction was significantly elevated. The activity of the marker enzyme - glutamine synthetase (GS) was also significantly increased in the GPV fraction. The activation of glial functions suggest a regulatory role for these cells in the early period of acute lead toxicity.

Ograniczanie wyników

1 Acta Neurobiologiae Experimentalis

1 Cellular and Molecular Biology Letters

1 Berner A.

1 Berner J.

1 Brocki M.

1 Jakubik J.

1 Kilianska Z.

1 Krajewska W. M.

1 Rajczyk B.

1 Struzynska L.

1 Szymczyk P.

1 2000

1 1996

Wyniki wyszukiwania

Immunospecific nuclear proteins of colorectal tumors

The protective role of astroglia in the early period of experimental lead toxicity in the rat