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DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

100%

Takahashi H., Yamaguchi T., Koga M., Kageura H., Terada S.

Cellular and Molecular Biology Letters

2004

tom 09

nr 3

Previously, we demonstrated that when mouse erythroleukemia cells are exposed to a pressure of 80 MPa, the cell-cycle progression of S-phase cells is retarded. To examine the effects of high pressure on DNA replication, we used a Xenopus cell-free system. From cell-cycle progression of sperm nuclei, it was found that sperm nuclei are stable to a pressure of 80 MPa, whereas egg extracts are susceptible to high pressure. Similarly, biotin-16-dUTP was incorporated into 80 MPa-treated sperm nuclei in pressure-untreated extracts, but not into naive sperm nuclei in 80 MPa-treated extracts. These results indicate that DNA replication in Xenopus cell-free system is suppressed by the susceptibility of the extracts to a pressure of 80 MPa.

DNA damage and apoptosis induction in L1210 cells by cis-diamminedichloroplatinum[II] and its new aminoflavone analogue

72%

Ciesielska E., Studzian K., Zyner E., Ochocki J., Szmigiero L.

Cellular and Molecular Biology Letters

2000

tom 05

nr 2

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells

58%

Trapp-Fragnet L., Marie-Egyptienne D. T., Fakhoury J., Rasschaert D., Autexier C.

Cellular and Molecular Biology Letters

2012

tom 17

nr 4

The minimal vertebrate telomerase enzyme is composed of a protein component (telomerase reverse transcriptase, TERT) and an RNA component (telomerase RNA, TR). Expression of these two subunits is sufficient to reconstitute telomerase activity in vitro, while the formation of a holoenzyme comprising telomerase-associated proteins is necessary for proper telomere length maintenance. Previous reports demonstrated the high processivity of the human telomerase complex and the interspecies compatibility of human TERT (hTERT). In this study, we tested the function of the only known viral telomerase RNA subunit (vTR) in association with human telomerase, both in a cell-free system and in human cells. When vTR is assembled with hTERT in a cell-free environment, it is able to interact with hTERT and to reconstitute telomerase activity. However, in human cells, vTR does not reconstitute telomerase activity and could not be detected in the human telomerase complex, suggesting that vTR is not able to interact properly with the proteins constituting the human telomerase holoenzyme.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

1 Autexier C.

1 Ciesielska E.

1 Fakhoury J.

1 Kageura H.

1 Koga M.

1 Marie-Egyptienne D. T.

1 Ochocki J.

1 Rasschaert D.

1 Studzian K.

1 Szmigiero L.

1 Takahashi H.

1 Terada S.

1 Trapp-Fragnet L.

1 Yamaguchi T.

1 Zyner E.

1 2012

1 2004

1 2000

Wyniki wyszukiwania

DNA replication reaction in Xenopus cell-free system is suppressed by high pressure

DNA damage and apoptosis induction in L1210 cells by cis-diamminedichloroplatinum[II] and its new aminoflavone analogue

The human telomerase catalytic subunit and viral telomerase RNA reconstitute a functional telomerase complex in a cell-free system, but not in human cells