Wyniki wyszukiwania

1

The molecular basis of resistance to apoptosis in calcitriol differentiated cells

100%

Chrobak A.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

2

Ultrastructural changes in the cells of rhizomes of Polypodium vulgare L. after consecutive dehydrations

86%

Bagniewska-Zadworna A., Zenkteler E.

Cellular and Molecular Biology Letters

|

2002

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tom 07

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nr Suppl.

3

Effects of p53 inhibitor on survival and death of cells subjected to oxidative stress

72%

Strosznajder R. P., Jesko H., Banasik M., Tanaka S.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

|

nr 4

215-221

Our previous data indicate that ischemia and amyloid beta peptide (Abeta) cause an oxidative damage to macromolecules. In the present study we investigated the role of p53 protein in cell survival and death after administration of Abeta. The experiments were carried out on pheochromocytoma cells (PC-12) and cortical primary neurons in culture. The cortical neurons were exposed (48 h, 10 µM) to the action of a short Abeta25-35 neurotoxic fragment and the involvement of p53 was evaluated after addition of the p53 inhibitor pifithrin-alpha. Changes in cell morphology were evaluated by 4', 6-diamidino-2-phenylindole staining and the concentration-dependent effect of pifithrin-alpha on cells viability was determined. Additionally, we studied the effect of pifithrin-alpha on neuronal survival in vivo after a 5-min global brain ischemia followed by 7 days' reperfusion in gerbils. We found that Abeta enhanced apoptotic cell death in cortical primary neurons. Pifithrin-alpha, at a 10 µM final concentration, protected the neuronal cells from the apoptotic death. However, at concentrations of 0.1 and 1 mM, the p53 inhibitor decreased PC-12 cells' viability in a dose-dependent manner. In in vivo experiments we did not observe any neuroprotection by pifithrin-alpha in the CA1 hippocampal layer, which suggests that its effects strongly depend on the duration and type of an ischemic insult. Our data indicate that pifithrin-alpha affects neuronal cells in a dual manner. It has a protective effect at a low concentration, but becomes neurotoxic at higher concentrations.

4

The effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells

72%

Limaye V., Vadas M. A., Pitson S. M., Gamble J. R.

Cellular and Molecular Biology Letters

|

2009

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tom 14

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nr 3

411-423

The enzyme sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which is an important survival factor for endothelial cells (EC). Modest increases in intracellular SK1 activity in the EC are known to confer a survival advantage upon the cells. Here, we investigated the effects of more dramatic increases in intracellular SK1 in the EC. We found that these cells show reduced cell survival under conditions of stress, enhanced caspase-3 activity, cell cycle inhibition, and cell-cell junction disruption. We propose that alterations in the phosphorylation state of the enzyme may explain the differential effects on the phenotype with modest versus high levels of enforced expression of SK1. Our results suggest that SK1 activity is subject to control in the EC, and that this control may be lost in conditions involving vascular regression.

5

Study of cell survival in non-adherent conditions

72%

Malecki M., Sroczynska P., Janik P.

Cellular and Molecular Biology Letters

|

2000

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tom 05

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nr 2

6

mTOR and p70 S6K phosphorylation by insulin plus rapamycin in HepG2 cells with and without overexpression of constitutively active Akt-PKB

72%

Varma S., Gupta D., Khandelwal R. L.

Cellular and Molecular Biology Letters

|

2005

|

tom 10

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nr Suppl.2

7

Inhibitor of differentiation 1 [ID1] promotes cell survival and proliferation of prostate epithelial cells

58%

Schmidt M., Asirvatham A. J., Chaudhary J.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 2

Id1 (inhibitor of differentiation 1) is a member of the bHLH protein family. Consistent with its role in promoting proliferation and inhibiting differentiation, Id1 expression is low or negligible in normal prostate epithelial cells but is high in prostate cancer. Ectopic expression of Id1 in normal prostate epithelial cells could therefore provide a model for understanding early events involved in initiation of prostate cancer. Over-expression of Id1 immortalized but did not transform ventral prostate epithelial cells (Id1-RPE). Immortalization was associated with decreased Cdkn2a, Cdkn1a, androgen receptor and increased Tert expression. Gene expression profiling over successive doublings was used to identify transcriptomic changes involved during immortalization (Tieg, Jun, alpha actin, Klf10, Id2) and in maintaining the immortalized phenotype (Igfbp3, Igfbp5, Mmp2, Tgfb3). Network analysis indicated that Id1 promotes cancer/tumor morphology, cell cycle and epithelial to mesenchymal transition by influencing AP1, tnf, tgfβ, PdgfBB and estradiol pathways. During immortalization, the expression of majority of differentially expressed genes reduced over progressive doublings suggesting a decline in transcriptional regulatory mechanisms. The associated molecular/gene expression profile of Id1-RPE cells provides an opportunity to understand the molecular pathways associated with prostate epithelial cell survival and proliferation.

Ograniczanie wyników

6 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology. Supplement

1 Asirvatham A. J.

1 Bagniewska-Zadworna A.

1 Banasik M.

1 Chaudhary J.

1 Chrobak A.

1 Gamble J. R.

1 Gupta D.

1 Janik P.

1 Jesko H.

1 Khandelwal R. L.

1 Limaye V.

1 Malecki M.

1 Pitson S. M.

1 Schmidt M.

1 Sroczynska P.

1 Strosznajder R. P.

1 Tanaka S.

1 Vadas M. A.

1 Varma S.

1 Zenkteler E.

1 2010

1 2009

2 2005

2 2002

1 2000

The molecular basis of resistance to apoptosis in calcitriol differentiated cells

Ultrastructural changes in the cells of rhizomes of Polypodium vulgare L. after consecutive dehydrations

Effects of p53 inhibitor on survival and death of cells subjected to oxidative stress

The effects of markedly raised intracellular sphingosine kinase-1 activity in endothelial cells

Study of cell survival in non-adherent conditions

mTOR and p70 S6K phosphorylation by insulin plus rapamycin in HepG2 cells with and without overexpression of constitutively active Akt-PKB

Inhibitor of differentiation 1 [ID1] promotes cell survival and proliferation of prostate epithelial cells