Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: carbohydrate-binding protein

Sortuj według:

Ogranicz wyniki do:

Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities

100%

Lam S. K., Ng T. B.

Acta Biochimica Polonica

2009

tom 56

nr 4

649-654

A hexameric 150-kDa lectin was isolated from dried Hibiscus mutabilis seeds using a chromatographic protocol that involved ion exchange chromatography on SP-Sepharose, and gel filtration on Superdex 75 and Superdex 200. The lectin was not adsorbed on SP-Sepharose and was eluted from the Superdex 75 column in the void volume. It was eluted in the first peak from Superdex 200. It was strongly adsorbed on DEAE-cellulose and Q-Sepharose and could not be easily desorbed. The hemagglutinating activity of the lectin, which was stable at pH 4-7 and up to 50oC, could be inhibited by 25 mM galactonic acid. This is the first report of a galactonic acid-binding lectin. It potently inhibited HIV-1 reverse transcriptase with an IC50 of 0.2 µM. It exhibited weak antiproliferative activity towards both hepatoma HepG2 cells (40% inhibition) and breast cancer MCF-7 cells (50% inhibition) at 100 µM concentration of the lectin. It did not inhibit mycelial growth of a number of fungi tested.

Galectin-3 as a multifunctional protein

80%

Krzeslak A., Lipinska A.

Cellular and Molecular Biology Letters

2004

tom 09

nr 2

Galectin-3 is a 31 kDa member of a growing family of b-galactosidebinding animal lectins. This protein is expressed in a variety of tissues and cell types and is mainly found in the cytoplasm, although, depending on cell type and proliferative state, a significant amount of this lectin can also be detected in the nucleus, on the cell surface or in the extracellular environment. Galectin-3 is secreted from cells by a novel and incompletely understood mechanism that is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. Galectin-3 exhibits pleiotropic biological function, playing a key role in many physiological and pathological processes.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Cellular and Molecular Biology Letters

1 Krzeslak A.

1 Lam S. K.

1 Lipinska A.

1 Ng T. B.

1 2009

1 2004

Wyniki wyszukiwania

Novel galactonic acid-binding hexameric lectin from Hibiscus mutabilis seeds with antiproliferative and potent HIV-1 reverse transcriptase inhibitory activities

Galectin-3 as a multifunctional protein