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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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In silico and in vitro cytotoxic effect of prodigiosin-conjugated silver nanoparticles on liver cancer cells (HepG2)

100%
El-Batal A. I., El-Hendawy H. H., Faraag A. H. I.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2017
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tom 98
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nr 3
2

Celecoxib increases retinoid sensitivity in human colon cancer cell lines

100%
Liu J. P., Wei H.-B., Zheng Z.-H., Guo W.-P., Fang J.-F.
Cellular and Molecular Biology Letters
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2010
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tom 15
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nr 3
440-450
Retinoid resistance has limited the clinical application of retinoids as differentiation-inducing and apoptosis-inducing drugs. This study was designed to investigate whether celecoxib, a selective COX-2 inhibitor, has effects on retinoid sensitivity in human colon cancer cell lines, and to determine the possible mechanism of said effects. Cell viability was measured using the MTT assay. Apoptosis was detected via Annexin-V/PI staining and the flow cytometry assay. PGE2 production was measured with the ELISA assay. The expression of RARβ was assayed via western blotting. The results showed that celecoxib enhanced the inhibitory effect of ATRA in both COX-2 high-expressing HT-29 and COX-2 low-expressing SW480 cell lines. Further study showed the ATRA and celecoxib combination induced greater apoptosis, but that the addition of PGE2 did not affect the enhanced growth-inhibitory and apoptosis-inducing effects of the combination. Moreover, NS398 (another selective COX-2 inhibitor) did not affect the inhibitory effects of ATRA in the two cell lines. Western blotting showed that the expression of RARβ in HT-29 cell lines was increased by celecoxib, but not by NS398, and that the addition of PGE2 did not affect the celecoxib-induced expression of the retinoic acid receptor beta. In conclusion, celecoxib increased the expression of RARβ and the level of cellular ATRA sensitivity through COX-2-independent mechanisms. This finding may provide a potential strategy for combination therapy.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Synthesis and in vitro anticancer activity of N-alkyl phosphoramidate monoesters of 3N-azido-3N-deoxythymidine (AZT)

100%
Czajkowska-Wojciechowska E., Singh A., Trznadel R., Ruszkowski P., Celewicz L.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2019
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tom 100
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nr 1
4
Content available remote

The lactate receptor (HCAR1/GPR81) contributes to doxorubicin chemoresistance via ABCB1 transporter up-regulation in human cervical cancer HeLa cells

100%
Wagner W., Kania K. D., Blauz A., Ciszewski W. M.
Journal of Physiology and Pharmacology
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2017
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tom 68
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nr 4
5

Secretomic analysis of large cell lung cancer cell lines using two-dimensional gel electrophoresis coupled to mass spectrometry

100%
Yousefi Z., Sarvari J., Nakamura K., Kuramitsu Y., Ghaderi A., Mojtahedi Z.
Folia Histochemica et Cytobiologica
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2012
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tom 50
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nr 3
6

The effect of tyrphostins AG494 and AG1478 on the autocrine growth regulation of A549 and DU145 cells

100%
Bojko A., Reichert K., Adamczyk A., Ligeza J., Ligeza J., Klein A.
Folia Histochemica et Cytobiologica
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2012
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tom 50
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nr 2
7
Content available remote

Transcriptomic profile of two canine mammary cancer cell lines with different proliferative and anti-apoptotic potential

100%
Krol M., Pawlowski K. M., Skierski J., Rao N. A. S., Hellmen E., Mol J. A., Motyl T.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
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nr 1
95-106
The aim of the study was to identify the genes responsible for the high growth rate and anti-apoptotic potential in selected canine mammary cancer cells. cDNA canine microarrays were used to compare the transcriptome in simple carcinoma CMT-U27 and spindle-cell tumor CMT-U309 cell lines. In CMT-U27 cell line the growth rate (shorter cell cycle), anti-apoptotic potential (higher expression of Bcl-2) was higher and spontaneous and induced apoptosis was lower. Comparison of transcriptomes revealed 333 genes which expression differed similarly. We focused on genes involved in cell proliferation, adhesion and apoptosis, and selected 29 of them. The high growth rate and anti-apoptotic potential in CMT-U27 cells was associated with enhanced expression of genes (at the level of transcripts) involved in Ca2+ signaling pathway (Calmodulin 1, 2, 3 and SPSB2) and growth hormone cellular pathway. The low-proliferative and pro-apoptotic phenotype of CMT-U309 cells was more dependent on TGFß, neuregulin 1 pathways and adhesion-related molecules.
8

Capsaicin induces apoptosis by generating reactive oxygen species and disrupting mitochondrial transmembrane potential in human colon cancer cell lines

100%
Yang K. M., Pyo J. O., Kim G.-Y., Yu R., Han I. S., Ju S. A., Kim W. H., Kim B.-S.
Cellular and Molecular Biology Letters
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2009
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tom 14
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nr 3
497-510
Although genetic factors are a well-known cause of colorectal cancer, environmental factors contribute more to its development. Despite advances in the fields of surgery, radiotherapy and chemotherapy, the cure rates for colon cancer have not substantially improved over the past few decades. Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), the principal pungent ingredient of hot chili pepper, has exhibited an anti-tumor effect in many cell types. However, the mechanisms responsible for the anti-tumor effect of capsaicin are not yet completely understood. In this study, we investigated whether capsaicin induces apoptosis in colon cancer cell lines. Capsaicin decreased cell viability in a dose-dependent manner in Colo320DM and LoVo cells. In addition, capsaicin produced cell morphology changes and DNA fragmentation, decreased the DNA contents, and induced phosphatidylserine translocation, which is a hallmark of apoptotic cell death. We showed that capsaicin-induced apoptosis is associated with an increase in ROS generation and a disruption of the mitochondrial transmenbrane potential. A possible mechanism of capsaicin-induced apoptosis is the activation of caspase 3, a major apoptosis-executing enzyme. Treatment with capsaicin induced a dramatic increase in caspase 3 activity, as assessed by the cleavage of Ac-DEVD-AMC, a fluorogenic substrate. In conclusion, our results clearly showed that capsaicin induced apoptosis in colon cancer cells. Although the actual mechanisms of capsaicin-induced apoptosis remain uncertain, it may be a beneficial agent for colon cancer treatment and chemoprevention.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

80%
El-Gendy M. M. A. A., Mohamed Z. K., Hekal N. Z., Ali F. M., Yousef A. E. M.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2018
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tom 99
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nr 1
10

The structural and functional analysis of the human HSPA2 gene promoter region

80%
Piglowski W., Nowak R., Krawczyk Z., Scieglinska D.
Acta Biochimica Polonica
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2007
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tom 54
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nr 1
HSPA2 is a human counterpart of the testis-specific rodent Hst70/Hsp70.2 gene. In contrast to the latter, the expression of the human HSPA2 gene is not limited to the testis, and recent data show that human tumor cells can express this gene at significant levels. The characteristics of HSPA2 expression suggests that it can influence the phenotype and survival of cancer cells similarly as overexpression of major members of the HSP70 gene family. Until now, neither the structure of the transcription unit of the human HSPA2 gene has been established nor a functional analysis of its promoter performed. In this study we established that the human HSPA2 gene, in contrast to its rodent counterparts, is intronless and has a single transcription start site. We also show that the same type of HSPA2 transcripts are synthesized in the testes and in cancer cell lines. In order to perform a functional study of the HSPA2 promoter, we used a transient transfection assay and found that the 392 bp fragment upstream of the ATG codon was a minimal region required for efficient transcription, while a 150 bp deletion from the 5' end of this region dramatically reduced the promoter activity. Delineation of the minimal promoter is a basic step toward identifiying the cis and trans elements involved in the regulation of the HSPA2 gene expression in cancer cells.
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