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Detection of bovine foamy virus in milk and saliva of BFV seropositive cattle

100%

Materniak M., Sieradzki Z., Kuzmak J.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 4

The aim of this study was to demonstrate the oceurrence of bovine foamy virus (BFV) and BFV DNA in peripheral blood leukocytes (PBLs), milk cells, and saliva of cattle serologically positive to BFV. The virus was detected by co-cultivation technique with canine thymus cells (Cf2Th) and viral DNA was quantified by the real-time PCR. Out of co-cultures from 23 cattle, BFV was found in 19, 13, and 15 samples of PBLs. milk cells and saliva, respectively, while BFV-DNA was confirmed in DNA extracted from 14 PBLs and 8 milk cells samples. All 23 saliva samples were negative. The number of BFV DNA copies in milk cells was in average over 2.5 times lower than in PBLs. The presence of BFV and viral DNA in milk and saliva samples is discussed in the context of virus spread and possible human exposure to BFV through food products of cattle origin.

Isolation and partial characterization of bovine foamy virus from Polish cattle

100%

Materniak M., Bicka L., Kuzmak J.

Polish Journal of Veterinary Sciences

2006

tom 09

nr 4

The first isolation and partial characterization of bovine foamy virus (BFV), also known as bovine syncytial virus, in Poland is described. This virus was isolated by co-cultivation of peripheral blood leukocytes from infected cattle with permissive Cf2Th cells. The new isolate, called BFV100 was identified using several techniques: electron microscopy, western blotting, PCR and sequencing of a part of the gag and pol/env genes. Based on syncytia induction, antigenic determinants, primer binding sites and sequence analysis, it can be concluded that isolate BFV100 is bovine foamy virus and is related to the known American and German BFV isolates by sequence homology and antigenic relatedness.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

2 Kuzmak J.

2 Materniak M.

1 Bicka L.

1 Sieradzki Z.

1 2010

1 2006

Wyniki wyszukiwania

Detection of bovine foamy virus in milk and saliva of BFV seropositive cattle

Isolation and partial characterization of bovine foamy virus from Polish cattle