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Background. For proper construction and functioning of human skeletal system a very important thing is adequate supply of calcium, which content in daily rations, in addition to genetics, degree of physical activity and level of sex steroids, is an essential factor influencing on bone mass. Aim of the work: the aim of the work was to assess mineral status of regular soldiers doing military service in different types of Polish military units. Material and methods. An assessment of bones calcification and protein-energy nutritional status of 1913 men, soldiers doing military service in different types of Polish military units, was made. Body height and body mass were determined by standard methods using a scales and a height-meter. Bone mineral density was measured by DEXA densitometry on forearm bone of non-prevailing arm, using the EXA 3000 apparatus. Results. Results of densitometry showed that 1594 soldiers, that is 83.3% of subjects had standard bone calcification. Bone mineralization characteristic of osteopenia was found among 304 people, that is 15.9% of examined, while 15 subjects (0.8%) revealed changes characteristic of osteoporosis. Conclusions. 1. Bone mineral density of 16.7% of examined soldiers serving in different types of military units indicates presence of abnormalities in bone calcification with varying degrees of severity. 2. It is advisable to take among soldiers an extensive health promotion regarding dietary health education aimed at nutritional prevention of bone mineralization disorders can cause fractures and early elimination soldiers from service.
 Glucocorticoids and β2-adrenergic receptor agonists are the most commonly used drugs in the treatment of asthma. Both therapies are potentially dangerous to the skeletal system. The aim of the present study was to investigate the effects of fenoterol, a β2-receptor agonist, on the development of bone changes induced by glucocorticoid (prednisolone) administration in mature male rats. The experiments were carried out on 24-week-old male Wistar rats. The effects of prednisolone 21-hemisuccinate sodium salt (7 mg/kg s.c. daily) or/and fenoterol hydrobromide (1.4 mg/kg i.p. daily), administered for 4 weeks, on the skeletal system were studied. Bone turnover markers, geometric parameters, mass, mass of bone mineral in the tibia, femur and L-4 vertebra, bone histomorphometric parameters and mechanical properties of tibial metaphysis, femoral diaphysis and femoral neck were determined. Both prednisolone and fenoterol had damaging effects on the skeletal system of mature male rats. However, concurrent administration of fenoterol and prednisolone did not result in the intensification of the deleterious skeletal effect of either drug administered separately.
Genistein, a major phytoestrogen of soy, is considered a potential drug for prevention and treatment of postmenopausal osteoporosis. The aim of the present study was to compare the effects of genistein, estradiol and raloxifene on the skeletal system in vivo and in vitro. Genistein (5 mg/kg), estradiol (0.1 mg/kg) or raloxifene hydrochloride (5 mg/kg) were administered daily by a stomach tube to mature ovariectomized Wistar rats for 4 weeks. Bone mass, mineral and calcium content, macrometric parameters and mechanical properties were examined. Also the effects of genistein, estradiol and raloxifene (10-9-10-7 M) on the formation of osteoclasts from neonatal mouse bone marrow cells and the activity of osteoblasts isolated from neonatal mouse calvariae were compared. In vivo, estrogen deficiency resulted in the impairment of bone mineralization and bone mechanical properties. Raloxifene but not estradiol or genistein improved bone mineralization. Estradiol fully normalized the bone mechanical properties, whereas genistein augmented the deleterious effect of estrogen-deficiency on bone strength. In vitro, genistein, estradiol and raloxifene inhibited osteoclast formation from mouse bone marrow cells, decreasing the ratio of RANKL mRNA to osteoprotegerin mRNA expression in osteoblasts. Genistein, but not estradiol or raloxifene, decreased the ratio of alkaline phosphatase mRNA to ectonucleotide pyrophosphatase phosphodiesterase 1 mRNA expression in osteoblasts. This difference may explain the lack of genistein effect on bone mineralization observed in ovariectomized rats in the in vivo study. Concluding, our experiments demonstrated profound differences between the activities of genistein, estradiol and raloxifene towards the osseous tissue in experimental conditions.
Three phytase preparations were added to broiler rations with considerably reduced levels of phosphorus. Birds were allocated to a positive control group (I), a negative control group (II) and three phytase-supplemented groups (III – Aspergillus niger phytase, IV – Penicillium canescens phytase, V – Pichia phytase). Major components of starter and grower diets were wheat, corn and soybean meals. Starter and grower diets (in meal form) contained 6.65 or 6.01 g of total P/kg and 3.98 or 3.68 g available P/kg in group I, and 5.67 or 5.05 of total P/kg and 2.98 g or 2.70 g available P/kg in groups II – V, respectively. This experiment, performed over a five-week period, involved Ross 308 male chickens kept in battery cages. Each group was divided into 9 subgroups, each of 9 chickens (81 birds per treatment). Phytase efficacy was evaluated based on performance results, carcass quality and bone mineralization. Optimal performance levels were achieved in group I (2.149 g body weight, 1.776 kg feed/kg gain). P reduction in group II decreased weight gains by 9% and increased feed conversion by about 5%. Diet supplementation with phytase in groups III – V compensated for the decrease in performance observed in group II. The effect exerted by three microbial phytases was similar. The difference in carcass weight between group II and the other groups was significant. P reduction in the diets negatively influenced the process of bone mineralization, which was enhanced by phytase supplementation.
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