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Activation of blood neutrophils in horses with the use of different doses of endotoxin

100%

Krumrych W., Wisniewski E., Dabrowska J., Danek J.

Bulletin of the Veterinary Institute in Puławy

1997

tom 41

nr 1

The aim of the study was to determine the influence of various doses of lipopolysaccharides (LPS) on the phagocytic activity of neutrophils in 12 clinically healthy mares. Three mares were regarded as the controls; the experimental groups consisted of the remaining animals divided into 3 groups of 3 mares each. All experimental animals were treated with intravenous infusion at doses of 0.02, 0.05, and 0.2 μg LPS/kg b.w. Clinical examinations and blood collecting were performed directly before endotoxin injection and hourly for the first 8 h and 24, 48, 72, and 96 h thereafter. The results of clinical and haematological examinations of the experimental animals were typical for endotoxemic horses. The range of clinical value alterations was dependent on the LPS doses. An increase in the percentage of phagocytic cells, phagocytic indexes and NBT reduction was found in all experimental horses. The increases remained unchanged from a few to 96 h with respect to the doses used. The highest phagocytic activity of neutrophils was revealed in horses injected with a dose of 0.05 μg LPS/ kg b.w.

Changes in the phagocytic cells in children treated with continuous ambulatory peritoneal dialysis

100%

Wasik M., Blaim M., Kolewska D., Janota-Krawczyk E., Tomaszewska-Panczyk M., Sieniawska M.

Archivum Immunologiae et Therapiae Experimentalis

1997

tom 45

nr 2-3

Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide

80%

Wojcik R.

Polish Journal of Veterinary Sciences

2010

tom 13

nr 1

181-188

The objective of this study was to determine the effect of ß-l,3/l,6-D-glucan (Biolex-Beta HP) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in rats intoxicated by cyclophosphamide. The experimental material comprised 40 adult Wistar rats aged 14 weeks, divided into two equal groups, a control group and an experimental group, each of 20 adult rats, including 10 males and 10 females. In the course of 3 successive days, 20 rats from the experimental group were administered cyclophosphamide intramuscularly at a dose of 50 mg/kg body weight/day. On the 8th day of the experiment, 10 control group (K) rats and 10 experimental group (C) rats were sacrificed. Arterial blood samples were collected and diluted with heparin to determine and compare the phagocytic activity (Phatogest) and oxidative metabolism (Bursttest) of peripheral blood granulocytes and monocytes by flow cytometry. Starting on the 8lh day of the experiment, the feed of the remaining 10 rats from the experimental group (C+G) and 10 rats from the control group (G) was supplemented with Biolex-Beta HP at a dose of 50 mg/kg body weight/day for 14 consecutive days. On day 22, arterial blood samples were collected from all (C+G) and (C) group rats, diluted with heparin to determine and compare the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes by flow cytometry. The results showed that Biolex-Beta HP modulated the phagocytic activity and oxidative metabolism of blood neutrophils and monocytes suppressed by cyclophosphamide in rats. The immunorestoring activity of Biolex-Beta HP was observed in this study.

Ograniczanie wyników

1 Archivum Immunologiae et Therapiae Experimentalis

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

1 Blaim M.

1 Dabrowska J.

1 Danek J.

1 Janota-Krawczyk E.

1 Kolewska D.

1 Krumrych W.

1 Sieniawska M.

1 Tomaszewska-Panczyk M.

1 Wasik M.

1 Wisniewski E.

1 Wojcik R.

1 2010

2 1997

Wyniki wyszukiwania

Activation of blood neutrophils in horses with the use of different doses of endotoxin

Changes in the phagocytic cells in children treated with continuous ambulatory peritoneal dialysis

Effect of Biolex Beta-HP on phagocytic activity and oxidative metabolism of peripherial blood granulocytes and monocytes in rats intoxicated by cyclophosphamide