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This research was conducted to determine the effects of three grafting methods (chip budding, side and splice grafting) and topical applications on graft scion of IBA (0 and 4000 ppm) and three plant growth promoting rhizobacteria (PGPR) strains (Bacillus subtilus-OSU142, Bacillus megatorium-M3 and Burkholderia gladia-BA7) on graft success and plant growth in black mulberry sapling production. The experiment was carried out in a randomized complete block design with three replications in Bolu, Turkey in 2014 and 2015. The black mulberry (Morus nigra L.) were used for grafting scions. Twoyear old white mulberry (Morus alba L.) seedling were used for rootstocks. The results showed that in general all of the bacterial strains and IBA had significant effects on all parameters tested in graft scion compared with the control. Application of 4000 ppm IBA and Bacillus megatorium-M3 increased the success graft take rate (74.44 and 72.22%, respectively), graft sprouting rate (61.11 and 60.00%, respectively), graft shoot diameter (6.21 and 5.70 mm, respectively) and graft shoot length (35.50 and 35.31 cm, respectively). Grafting methods had significant effect for all parameters and grafting methods increased the graft success. The best graft take rates (75.33 and 70.67%, respectively), graft sprouting rates (62.67 and 57.33% respectively), graft shoot diameter (5.56 and 5.88 mm, respectively) and graft shoot length (34.14 and 37.86 cm, respectively) were obtained from splice grafting and chip budding methods. In conclusion, the present study showed that 4000 ppm IBA and PGPR strains (especially M3 and OSU142) increased the graft success of black mulberry graft. The PGPR application may be of benefit for grafting in mulberry cultivars, particularly for organic farming. Besides, splice grafting and chip budding were found to be successful to black mulberry plant production.
Experiments were done to determine the effects of cold stratification (0, 20, 40, 60, 80 or 100 days), application of gibberelic acid (GA3) (0, 250, 500, 1000 or 2000 mg/l) and the combination (GA3 + stratification) on seed germination of black mulberry. Application of 1000 mg/l GA3 proved more effective than any of the other concentrations of GA3 applied. Seeds stratified for 100 days showed 88% germination. The combined treatment of 250 mg/l GA3 and 100 days of stratification yielded 96% germination of seeds. The relationships between GA3 concentration and seed germination (r = 0.93), and between stratification duration and seed germination (r = 0.91) of black mulberry were linear.
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