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Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

100%

Kurnatowska A., Bialasiewicz D.

Wiadomości Parazytologiczne

2001

tom 47

nr 4

Hydrolase activity of (enzymograms, biotypes) in Geotrichum candidum, one of the poorly described pathogenic fungi, was studied 81 strains were isolated from oral cavity and faeces of patients with gastrointestinal tract disorders. Axenic strains were differentiated with API 20C Aux and API ZYM tests. Then, enzymograms and biotypes were determined for all strains based on the activity of 19 hydrolases. High variability of enzymograms (17 different types) was found. The highest activity was noted in case of: e2-alkaline phosphatase, e6-lecucine arylamidase, e11-acid phosphatase. E5-lipase. e7-valine arylamidase, e12 - naphtol-AS-BI-phosphohydrolase and e17-β-glucosidase were used for biotyping procedures. Our own system of biotyping of 81 strains of G. candidum was based on the mathematical binominal distribution formula (1 : 4 : 6 : 4: 1) – all; ,,+”;one „-”, three „+”; two „-”,two „+”; three ,,-”,one ,,+”; all „-”. We have found: A (11.1 ± 3.5%), B1 (6.17 ± 2.67%). B2 (1.23 ± 1.22%), B4 (4.94 ± 2.41%), C1 (1.23 ±1.22%), C3 (63.0 ± 5.4%), D2 (9.88 ±3.31%), D3 (2.47 ± 1.72%). Among all strains from 8 various biotypes of G. candidum.

Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs

72%

Bancerz-Kisiel A., Szczerba-Turek A., Platt-Samoraj A., Szweda W.

Bulletin of the Veterinary Institute in Puławy

2011

tom 55

nr 1

The study has been taken up to collect more data on Yersinia enterocolitica isolated from pigs as the main reservoir and source of infection with strains pathogenic for humans. Bacteriological examinations, bio- and serotyping were conducted on 616 rectal swabs, taken from 308 fattening pigs. Two samples were taken from each animal to determine the ability of Y. enterocolitica to grow under different temperature conditions (warm and cold culture). It has been proven that low temperature constitutes a suitable culture condition. 138 Yersinia enterocolitica strains were isolated (22.40%), most of which (65.22%) were obtained in cold culture and 99.28% included in biotype 3 (one strain belonged to biotype 2). Serotyping yielded a positive result in 107 strains with the diagnostic serum for antigen O:3, in 18 - with the serum for antigen O:9, and 13 strains were determined to be non-typable. The results indicated that asymptomatic infections with Y. enterocolitica strains of the biotypes and serotypes pathogenic for humans are common in pig population.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Wiadomości Parazytologiczne

1 Bancerz-Kisiel A.

1 Bialasiewicz D.

1 Kurnatowska A.

1 Platt-Samoraj A.

1 Szczerba-Turek A.

1 Szweda W.

1 2011

1 2001

Wyniki wyszukiwania

Geotrichum candidum Link 1809: hydrolases activity and own methoof digestive tract strains biotyping

Isolation, biotyping, and serotyping of Yersinia enterocolitica strains from fattening pigs