Wyniki wyszukiwania

1

Beclin-1 and its role as a target for anticancer therapy

100%

Toton E., Lisiak N., Sawicka P., Rybczynska M.

Journal of Physiology and Pharmacology

|

2014

|

tom 65

|

nr 4

2

Molecular structure and biological function of von Willebrand factor

100%

Bykowska E.

Acta Biochimica Polonica

|

2007

|

tom 54

|

nr Supplement 4

3

Quasi-quantum effects in biological systems

100%

Molski M.

Current Topics in Biophysics. Supplement

|

2011

|

tom 34

|

nr A: Plenary lectures

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The correlation of structural features of mature miRNAs with their biological function

100%

Belter A., Naskret-Barciszewska M. Z.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2014

|

tom 95

|

nr 3

5

The role of probiotics in the organism

100%

Siuta A.

Acta Agraria et Silvestria. Series Zootechnica

|

1997

|

tom 35

33-45

6

Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application

100%

Lopatko K. G., Melnichuk M. D., Aftandilyants Y. G., Gonchar E. N., Boretskij V. F., Veklich A. N., Zakharchenko S. N., Tugay T. I., Tugay A. V., Trach V. V.

Annals of Warsaw University of Life Sciences - SGGW. Agriculture

|

2013

|

nr 61 Agric.Forest Eng.

Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application. The process of electrical discharge in the water of the plasma discharge channel was investigated and its diagnostics was performed. The specific features of the energy redistribution were shown, together with the physical model of the dispersed phase. The biological functionality of the metal nanoparticles and the prospects for their use in biotechnology were found.

7

Plant purple acid phosphatases - genes, structures and biological function

100%

Olczak M., Morawiecka B., Watorek W.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 4

The properties of plant purple acid phosphatases (PAPs), metallophosphoesterases present in some bacteria, plants and animals are reviewed. All members of this group contain a characteristic set of seven amino-acid residues involved in metal li- gation. Animal PAPs contain a binuclear metallic center composed of two irons, whereas in plant PAPs one iron ion is joined by zinc or manganese ion. Among plant PAPs two groups can be distinguished: small PAPs, monomeric proteins with molecular mass around 35 kDa, structurally close to mammalian PAPs, and large PAPs, homodimeric proteins with a single polypeptide of about 55 kDa. Large plant PAPs exhibit two types of structural organization. One type comprises enzymes with subunits bound by a disulfide bridge formed by cysteines located in the C-terminal region around position 350. In the second type no cysteines are located in this position and no disulfide bridges are formed between subunits. Differences in structural organisation are reflected in substrate preferences. Recent data reveal in plants the occurrence of metallophosphoesterases structurally different from small or large PAPs but with metal-ligating sequences characteristic for PAPs and expressing pronounced specificity towards phytate or diphosphate nucleosides and inorganic pyrophosphate.

8

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

84%

Luo Y., Tang Y., Xia Q., Liu J.

Cellular and Molecular Biology Letters

|

2007

|

tom 12

|

nr 4

595-603

Endothelin (ET), originally characterized as a vasoconstrictive peptide, has been found to have many different biological functions, including acting as a local hormonal regulator of pressure, fluid, ions and neurotransmitters in the inner ear. The objective of this study was to examine and quantify the mRNA expression of the endothelin type A and B receptors (ETAR and ETBR) in the strial vascularies (StV) and non-strial tissues (NSt) of the cochlear lateral wall using the real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) technique. The mouse tissue samples were harvested and RNA was extracted. RT was performed to obtain cDNA, and then the mRNA expression of each gene was measured via real-time PCR. We found that both receptor subtypes were expressed in the cochlear lateral wall, with a predominance of ETAR over ETBR. We showed that the mRNA expression of the two receptor subtypes was higher in the StV with a 1.8 times higher level of ETAR and an 8.1 times higher level of ETBR mRNAs than in the adjacent NSt of the lateral wall tissue. This study shows the existence and the quantity of ET receptor subtypes in the StV and NSt of the mouse cochlea. Our results suggest that an endothelin-mediated response via two different receptors, ETAR and ETBR, may play an important role in the physiological functions of the cochlear lateral wall by maintaining the homeostatic environment of the cochlea.

9

Regulation of lncRNA expression

84%

Wu Z., Liu X., Liu L., Deng H., Zhang J., Xu Q., Cen B., Ji A.

Cellular and Molecular Biology Letters

|

2014

|

tom 19

|

nr 4

Long non-coding RNAs (lncRNAs) are series of transcripts with important biological functions. Various diseases have been associated with aberrant expression of lncRNAs and the related dysregulation of mRNAs. In this review, we highlight the mechanisms of dynamic lncRNA expression. The chromatin state contributes to the low and specific expression of lncRNAs. The transcription of non-coding RNA genes is regulated by many core transcription factors applied to protein-coding genes. However, specific DNA sequences may allow their unsynchronized transcription with their location-associated mRNAs. Additionally, there are multiple mechanisms involved in the post-transcriptional regulation of lncRNAs. Among these, microRNAs might have indispensible regulatory effects on lncRNAs, based on recent discoveries.

10

H3K4 histone methylation in oral squamous cell carcinoma

84%

Mancuso M., Matassa D. S., Conte M., Colella G., Rana G., Fucci L., Piscopo M.

Acta Biochimica Polonica

|

2009

|

tom 56

|

nr 3

405-410

Methylation of specific lysine residues in histone tails has been proposed to function as a stable epigenetic marker that directs biological functions altering chromatin structure. Recent findings have implicated alteration in heterochromatin formation as a contributing factor in cancer development. In order to verify whether changes in the overall level of H3K4 histone methylation could be involved in oral squamous carcinoma, the levels of H3K4me1, me2 and me3 were measured in oral squamous carcinoma, leukoplakias and normal tissues. The levels of H3K4me2 and me3 were significantly different in oral squamous cell carcinoma in comparison with normal tissue: the level of H3K4me2 was increased while that of H3K4me3 decreased. No significant differences could be found between the two types of tissues in the level of H3K4me1. A similar trend was found in the leukoplakias that appeared more like the pathological than normal tissue. These results support the idea that alteration of chromatin structure could contribute to oncogenic potential

11

The role of carotenoids in fish

84%

Wozniak M.

Acta Academiae Agriculturae ac Technicae Olstenensis. Protectio Aquarum et Piscatoria

|

1996

|

tom 22

65-74

12

Regulation of bacterial protease activity

84%

Wladyka B., Pustelny K.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 2

212-229

Proteases, also referred to as peptidases, are the enzymes that catalyse the hydrolysis of peptide bonds in polipeptides. A variety of biological functions and processes depend on their activity. Regardless of the organism’s complexity, peptidases are essential at every stage of life of every individual cell, since all protein molecules produced must be proteolytically processed and eventually recycled. Protease inhibitors play a crucial role in the required strict and multilevel control of the activity of proteases involved in processes conditioning both the physiological and pathophysiological functioning of an organism, as well as in host-pathogen interactions. This review describes the regulation of activity of bacterial proteases produced by dangerous human pathogens, focusing on the Staphylococcus genus.

13

The tumor supressor function of STGC3 and its reduced expression in nasopharyngeal carcinoma

84%

He X. S., Deng M., Yang S., Xiao Z.-Q., Luo Q., He Z.-M., Hu B., Chen Z.-C.

Cellular and Molecular Biology Letters

|

2008

|

tom 13

|

nr 3

339-352

STGC3 is a novel candidate tumor suppressor gene that was found to be associated with nasopharyngeal carcinoma (NPC) via the cDNA cloning and RACE processes. The biological function of the STGC3 protein and its expression level in nasopharyngeal carcinoma remain unknown. This study aimed to evaluate the STGC3 protein expression level in NPC and to investigate the inhibitory function of STGC3 as a candidate tumor suppressor gene. We assessed the expression of the STGC3 protein in NPC biopsies and normal control specimens via Western blot and immunohistochemical analysis. The expression of STGC3 as induced by doxycycline (Dox) via a tetracycline (Tet)-regulated system in human nasopharyngeal carcinoma cell line CNE2 was also established, and the effect of STGC3 restoration on the biological behavior of CNE2 was observed. A reduced level of STGC3 expression (0.978 ± 0.213 versus 0.324 ± 0.185, P < 0.05) was detected in NPC versus normal nasopharyngeal tissue by Western blot assay. Immunohistochemical assays for STGC3 detected positive staining in the nuclei and cytoplasm of epithelial cells, and the positive expression rate in NPC, 8 of 21 (38%), was lower than that in normal nasopharynx samples, 16 of 22 (72%). After STGC3 expression was restored, the growth capacity and clone formation potential of CNE2 cells in soft agar were significantly suppressed, and the cell percentage in G0/G1 phase increased, while the percentage of cells entering the S and G2 phases decreased. This indicates that an abnormality in STGC3 expression is associated with nasopharyngeal carcinogenesis and that it may play an important role in controlling cell growth and regulating the cell cycle.

14

Docking for drug interface residues of modelled VPS33B of human with PtpA of Mycobacterium tuberculosis CDC1551

84%

Reddy K. M., Pattathil M. D., Jayachandra S. Y., Parameshwar A., Sulochana M. B.

International Letters of Natural Sciences

|

2014

|

tom 11

|

nr 2

VPS33B, a human Vacuolar Protein Sorting (VPS) protein which mediates the phagolysosomal fusion in macrophage of the eukaryotic organisms. This protein has a great role during the mycobacterial infections, which binds with the Mycobacterium protein tyrosine phosphatase A (PtpA). A single functional domain of PtpA has been identified using SMART domain databases, followed by finding the antigenicity of PtpA using CLC main workbench tool. The protein-protein interaction network predicts the interface of biological functions of proteins, built by using Cytoscape 2.8.3 version tool for manual literature survey of protein sets. According to the literature the specific interactivity of PtpA with VPS33B of human lead to pathogenesis, and provided a good platform to find the structure of VPS33B as it lacks the 3 dimensional structure in PDB. Homology Modelling of VPS33B provides a significant properties to design a specific drug through screening the drug databases (eDrug3D). The modelled protein has been validated through SAVES server maintained by NIH and UCLA with the standard Ramachandran plot with accuracy of 90.7 %. From our findings the interface residues are very crucial points which has been found through docking the modelled protein and Mycobacterium protein and interface residues were selected manually using PyMol software.

15

Developmental biology of Arabidopsis thaliana At1g67580 mutant

84%

Kuta E., Malec P., Pilarska M., Slomka A., Rataj K., Ilnicki T.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2008

|

tom 50

|

nr 1

16

Human diadenosine triphosphate hydrolase: preliminary characterization and comparison with the human tumor suppressor Fhit protein

84%

Rotllan P., Asensio A. C., Oaknin S.

Cellular and Molecular Biology Letters

|

1999

|

tom 04

|

nr 3

17

Thyroid hormone receptor alpha1: a switch to cardiac cell 'metamorphosis'?

84%

Pantos C., Xinaris C., Mourouzis I., Perimenis P., Politi E., Spanou D., Cokkinos D. V.

Journal of Physiology and Pharmacology

|

2008

|

tom 59

|

nr 2

Thyroid hormone receptor alphalpha1 (TRalpha1) is predominantly expressed in the myocardium but its biological function under physiological or pathological conditions remains largely unknown. The present study investigated possible interactions between alpha1 adrenergic and thyroid hormone signaling at the level of TRalpha1, potential underlying mechanisms and physiological consequences, as well as the role of TRalpha1 in cell differentiation. This may be of physiological relevance since both thyroid hormone and adrenergic signalling are implicated in the pathophysiology of cardiac remodelling. Neonatal cardiomyocytes obtained from newborn rats (2-3 days) were exposed to phenylephrine (PE, an alpha1 adrenergic agonist) for 5 days, in the absence or excess of T3 in the culture medium. PE, in the absence of T3, resulted in 5.0 fold increase in TRalpha1 expression in nucleus and 2.0 fold decrease in TRalpha1 expression in cytosol, P<0.05. As a result, a fetal pattern of myosin isoform expression with marked expression of ß–MHC was observed in PE treated vs the untreated cells, P<0.05. PD98059 (an ERK signalling inhibitor) abrogated this response. In the presence of T3 in the culture medium, TRalpha1 expression was increased 1.6 fold in nucleus and 2.0 fold in cytosol in PE-T3 vs PE treated cells, P<0.05, and the fetal pattern of myosin isoform expression was prevented. Parallel studies with H9c2 myoblasts showed that reduction of T3 binding to TRalpha1 receptor delayed cardiac myoblasts differentiation without affecting proliferation. In conclusion, in neonatal cardiomyocytes, nuclear TRalpha1 is overexpressed after prolonged activation of the alpha1- adrenergic signalling by PE. This response seems to be an ERK kinase dependent process. Over-expression of TRalpha1 may lead to fetal cardiac phenotype in the absence of thyroid hormone availability. Furthermore, TRalpha1 seems to be critical in cardiac myoblast differentiation.

18

Study on the biological function of Nicotiana tabacum osmotic stress activated protein kinase [NTOSAK]

84%

Pekala I., Kelner A., Dobrowolska G.

Biological Letters

|

2005

|

tom 42

|

nr 2 Spec.Vol.

19

Aleksander Koj's 50 years of academic achievement

84%

Bereta J., Dubin A.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 3

20

Physical properties of lipid bilayer membranes: relevance to membrane biological functions

84%

Subczynski W. K., Wisniewska A.

Acta Biochimica Polonica

|

2000

|

tom 47

|

nr 3

Over the last 25 years one of us (WKS) has been investigating physical properties of lipid bilayer membranes. In 1991 a group led by WKS was organized into the Laboratory of Structure and Dynamics of Biological Membranes, the effective member of which is AW. Using mainly the electron paramagnetic resonance (EPR) spin-labeling method, we obtained unexpected results, which are significant for the better understanding of the functioning of biological membranes. We have developed a new pulse EPR spin-labeling method for the detection of membrane domains and evaluation of lipid exchange rates. This review will be focused on our main results which can be summarized as follows: (1) Unsaturation of alkyl chains greatly reduces the ordering and rigidifying effects of cholesterol although the unsaturation alone gives only minor fluidizing effects, as observed by order and reorientational motion, and rather significant rigidifying effects, as observed by translational motion of probe molecules; (2) Fluid-phase model membranes and cell plasma membranes are not barriers to oxygen and nitric oxide transport; (3) Polar carotenoids can regulate membrane fluidity in a way similar to cholesterol; (4) Formation of effective hydrophobic barriers to the permeation of small polar molecules across membranes requires alkyl chain unsaturation and/or the presence of cholesterol; (5) Fluid-phase micro-immiscibility takes place in cis-unsaturated phosphatidylcholine-cholesterol membranes and induces the formation of cholesterol-rich domains; (6) In membranes containing high concentrations of transmembrane proteins a new lipid domain is formed, with lipids trapped within aggregates of proteins, in which the lipid dynamics is diminished to the level of gel-phase.

Ograniczanie wyników

Beclin-1 and its role as a target for anticancer therapy

Molecular structure and biological function of von Willebrand factor

Quasi-quantum effects in biological systems

The correlation of structural features of mature miRNAs with their biological function

The role of probiotics in the organism

Obtaining of metallic nanoparticles by plasma-erosion electrical discharges in liquid mediums for biological application

Plant purple acid phosphatases - genes, structures and biological function

The expression of endothelin type A and B receptors in the lateral wall of the mouse cochlea

Regulation of lncRNA expression

H3K4 histone methylation in oral squamous cell carcinoma

The role of carotenoids in fish

Regulation of bacterial protease activity

The tumor supressor function of STGC3 and its reduced expression in nasopharyngeal carcinoma