Wyniki wyszukiwania

1

Different beta-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis

100%

Surmacz L., Krawczyk K., Wyroba E.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 4

In Southern blot analysis of Paramecium genomic DNA using β-adrenergic specific oligonucleotides labelled with Digoxigenin-ddUTP, the DNA species of the same molecular size were detected by different molecular probes. The 6.5 kb DNA species hybridized with the probes directed to the adjacent β2-receptor transmembrane domains: to TM3 - including Asp 113 involved in β-agonists and antagonists binding, and to TM4 - the oligonucleotide being one of the "gene-specific universal mammalian sequence-tagged site”. Since a profound physiological effect of beta-agonists and antagonists has been previously observed in Paramecium, such a hybridization pattern may suggest an existence of Paramecium DNA sequences homologous to those of metazoan eukaryotes encoding for a membrane beta-adrenergic receptor.

2

The influence of beta-adrenergic receptors on uterus myoelectric activity in sheep sensitised with stilboestrol

86%

Dejneka J., Zieba D., Rauluszkiewicz S.

Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine

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1998

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tom 01

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nr 1

The research was conducted on 5 inter-breed hybrid sheep, at the age of 10 to 24 months and of the body mass of 30-45 kg, which had bipolar electrodes implanted to uterus horns and shank. The registration of uterus myoelectric activity was performed on sheep 24 and 48 hours after sensitising with Stilboestrol, by the use of Reega Duplex TR XVI electroencephalograph. After recording the output activity, the sheep were applied intravenously in continuous injection: adrenaline 0,12-0,18 μg ˇ kg-1 ˇ min-1, oxytocin 0,25-0,4 i.u., propranolol 1mg ˇ kg-1, isoprenaline 0,4-1,5 μg ˇ kg-1 ˇ min-1, salbutamole 0,4-0,8 μg ˇ kg-1 ˇ min-1. The experiment results are presented in the inclosed drawings. The conducted research revealed that the stimulation of beta-adrenergic receptors causes the decrease or inhibition of uterus myoelectric activity of sheep sensitised with Stilboestrol.

3

Characterization of the crude membrane fraction of cardiomyocytes using enzyme cytochemical markers

86%

Okruhlicova L., Tribulova N.

Folia Histochemica et Cytobiologica

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1993

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tom 31

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nr 3

4

PCR amplification of Paramecium DNA using the beta-adrenergic-specific-primers

86%

Wiejak J., Platek A., Surmacz L., Wyroba E.

Acta Protozoologica

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1998

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tom 37

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nr 4

5

Central beta-adrenergic receptors are involved in the mechanism of porphyrin-induced convulsive effect in mice

72%

Maksym B., Habdas J., Plech A.

Pestycydy

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2005

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nr 4

55-63

6

Beta-adrenergic antagonists influence abdominal aorta contractility by mechanisms not involving beta-adrenergic receptors

72%

Hauzer W., Bujok J., Czerski A., Rusiecka A., Pecka E., Gnus J., Zawadzki W., Witkiewicz W.

Folia Biologica

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2014

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tom 62

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nr 3

7

Pulmonary hypertension modifies responsiveness of sinoatrial cells of rat hearts to adrenomimetics and activators of ATP-sensitive Kplus channels in a gender-dependent way

72%

Kocic I., Sztormowska K.

Journal of Physiology and Pharmacology

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2009

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tom 60

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nr 1

165-169

The aim of this paper was to find out how pulmonary arterial hypertension (PAH) affects heart rate in males and females rats. Additionally, the concentration of tumor necrosis factor alpha (TNF-alpha) was monitored. Male and female rats were treated by monocrotaline (i.p., 60mg/kg B.W.) or by equivalent volume of normal saline solution (n=40) and after 4 weeks right auriculae containing sinoatrial cells have been isolated and examined. We have measured heart rate of spontaneously beating right auriculae and serum level of TNF-alpha. In females with PAH, isoprenaline curve was shifted to the left (pD2 increased from 10,9±3 to 15±3, n=10, P<0,05), but in males with PAH maximal acceleration of beating rate induced by isoprenaline decreased from 175±10 to 146±8 beats/min, P<0.05). Additionally, reduction in heart rate induced by rilmakalim was more significantly decreased in females (from -89±7 to - 49±3 beats/min, n=10, p<0.01) than in males (from - 68±6 to - 46±4 beats/min, P<0.05) with PAH. We did not detect any changes in TNF-alpha in any experimental group. Our results imply that pulmonary hypertension increased sensitivity of sinoatrial cells to isoprenaline and decreased efficacy of rilmakalim to reduce heart rate more significantly in females then in males.

8

RT-PCR and Northern blot analysis in search for a putative Paramecium beta-adrenergic receptor

72%

Platek A., Wiejak J., Wyroba E.

Acta Biochimica Polonica

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1999

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tom 46

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nr 3

RT-PCR and Northern blot analysis were performed in order to search for a putative beta-adrenergic receptor (β-AR) in Paramecium using several β2-adrenergic-specific molecular probes.Under strictly defined RT-PCR conditions DNA species of expected molecular size about 360 bp were generated with the primers corresponding to the universal mammalian β2-AR sequence tagged sites (located within the 4th and the 6th transmembrane regions of the receptor). This RT-PCR product hybridized in Southern blot analysis with the oligonucleotide probe designed to the highly conservative β2-AR region involved in G-proteins interaction and located within the amplified region. Northern hybridization was performed on Paramecium total RNA and mRNA with human β2-AR cDNA and two oligonucleotide probes: the first included Phe 290 involved in agonist binding (Strader et al., 1995) and the second was the backward RT-PCR primer. All these probes revealed the presence of about 2 kb mRNA which is consistent with the size of β2-AR transcripts found in higher eukaryotes.

9

Beta-adrenergic stimulation does not influence the activity of lymphocyte Kv1.3 channels

72%

Teisseyre A., Gawlik A., Krasnowska M.

Cellular and Molecular Biology Letters

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1997

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tom 02

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nr 3

The aim of our study was to clarify the still controversial problem concerning the modulatory effect of β-adrenergic stimulation on the activity of Kv1.3 channels in human T Lymphocytes. Because the expression of β-adrenergic receptors in T Lymphocytes is significantly altered in patients with bronchial asthma we examined the cells taken both from healthy donors and asthmatic patients. We applied the whole-cell patch-clamp technique to study the modulatory effect of β-adrenergic stimulation on the whole-cell potassium conductance, gating and kinetics of Lymphocyte Kv1.3 channels. During the experiments β-adrenergic agonist Isoprenaline was applied at concentrations up to 10-4 M. It was shown that the activity of T lymphocyte Kv1.3 channels remain unchanged upon β-adrenergic stimulation both in cells taken healthy donors and asthmatic patients. Results of our investigations support the notion that β- adrenergic stimulation does not modulate the activity of Kv1.3 channels in human TL. The transient increase in T lymphocyte K+ channel activity upon β-adrenergic stimulation that has been reported in some previous studies is most probably due to an activation of recently identified, voltage-independent cAMP-responsive K+ channels.

10

Characterization of beta-adrenergic receptors in duck cerebral cortex

72%

Zawilska J. B., Zalewska-Kaszubska J., Marczak G., Gorska D., Woldan-Tambor A.

Acta Neurobiologiae Experimentalis

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2000

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tom 60

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nr 3

11

Nonspecific effects of ligands on the beta-adrenergic receptors in rabbit abdominal aorta in vitro

58%

Gnus J., Czerski A., Bujok J., Ferenc S., Zawadzki W., Witkiewicz W., Hauzer W., Rusiecka A., Janeczek M.

Folia Biologica

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2013

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tom 61

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nr 3-4

12

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The neuromodulation aspects of ischaemic myocardium: the importance of cholinergic system

58%

Gajewski M., Moutiris J. A., Maslinski S., Ryzewski J.

Journal of Physiology and Pharmacology

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1995

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tom 46

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nr 2

13

Wplyw agonistow i antagonistow receptorow beta-adrenergicznych na aktywnosc skurczowa miesniowki macicy szczurow w badaniach in vitro

51%

Czerski A., Zawadzki W., Wincewicz E., Zawadzki M., Janeczek M., Wlodarczyk M.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2004

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tom 03

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nr 1

9-18

Ograniczanie wyników

Different beta-adrenergic-specific molecular probes reveal the same DNA species in Paramecium hybridization analysis

The influence of beta-adrenergic receptors on uterus myoelectric activity in sheep sensitised with stilboestrol

Characterization of the crude membrane fraction of cardiomyocytes using enzyme cytochemical markers

PCR amplification of Paramecium DNA using the beta-adrenergic-specific-primers

Central beta-adrenergic receptors are involved in the mechanism of porphyrin-induced convulsive effect in mice

Beta-adrenergic antagonists influence abdominal aorta contractility by mechanisms not involving beta-adrenergic receptors

Pulmonary hypertension modifies responsiveness of sinoatrial cells of rat hearts to adrenomimetics and activators of ATP-sensitive Kplus channels in a gender-dependent way

RT-PCR and Northern blot analysis in search for a putative Paramecium beta-adrenergic receptor

Beta-adrenergic stimulation does not influence the activity of lymphocyte Kv1.3 channels

Characterization of beta-adrenergic receptors in duck cerebral cortex

Nonspecific effects of ligands on the beta-adrenergic receptors in rabbit abdominal aorta in vitro

The neuromodulation aspects of ischaemic myocardium: the importance of cholinergic system

Wplyw agonistow i antagonistow receptorow beta-adrenergicznych na aktywnosc skurczowa miesniowki macicy szczurow w badaniach in vitro