Wyniki wyszukiwania

1

The biochemical diversity of heterotrophic bacterial cmmunities in high mountain lakes from Sierra Nevada (Granada, Spain)

100%

Canteras J.-C., Perez L.

Acta Hydrobiologica

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1987

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tom 29

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nr 2

2

Distribution of microbial-selected populations in Lake North Mamry by fluorescent in situ hybridization

100%

Skowronska A.

Polish Journal of Environmental Studies

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2007

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tom 16

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nr 1

Fluorescent in situ hybridization (FISH) with rRNA -targeted oligonucleotide probes has become one of the major techniques in environmental microbiology, allowing rapid and reliable definition of prokaryotes and quantification of population sizes. The aim was to demonstrate the applicability of the FISH method to study bacterioplankton composition in North Mamry Lake, and to follow the dynamics of two populations of common bacteria. We analyzed the phylogenetic composition of free-living bacterioplankton assemblage using oligonucleotide probes specific for Bacteria as well as for β-Proteobacteria and Cytophaga-Flavobacterium groups. Up to 53% of bacteria detected with DA PI could be detected via FISH by applying the universal bacterial probe for domain Bacteria (Eub338). Percentage of Cytophaga-Flavobacterium cluster did not exceed 20%. Members of the β –Proteobacteria appeared to be the most abundant group.

3

Molecular analysis of bacterial community diversity in sequencing batch reactor [SBR] operating in autotrophic conditions

86%

Ciesielski S., Cydzik-Kwiatkowska A., Turek J.

Polish Journal of Microbiology

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2007

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tom 56

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nr 1

In the present study the bacterial community structure in a sequencing batch reactor (SBR) operating in autotrophic conditions was determined in relation to temporal variations of NH₄-N, NO₂-N, NO₃-N levels in the effluent. Bacterial richness and composition were determined by PCR-DGGE (Polymerase Chain Reaction - Denaturing Gradient Gel Electrophoresis) of 16S rRNA and amoA genes, and RISA (Ribosomal Intergenic Spacer Analysis) technique. The applied approaches revealed that the composition of bacterial population in the studied SBR varied in time. A positive correlation between Shannon index of ammonia-oxidizing bacteria (AOB) diversity and ammonia level in the effluent was observed. The variation of total bacterial diversity did not reflect the changes in nitrification efficiency.

4

Bacterial community DNA extracted from soils polluted with heavy metals

72%

Kozdroj J., van Elsas J. D.

Polish Journal of Environmental Studies

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2000

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tom 09

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nr 5

403-407

This paper describes the application of two direct and one indirect methods for the extraction of microbial community DNA from soils polluted with heavy metals. DNA was extracted directly from soil by a gentle method based on the soil incubation at 37°C with proteinase K and SDS or the method was modified by the addition of bead beating step. The indirect approach was based on the RNA/DNA extraction method. The level of soil contamination did not affect on the yields of DNA extracted and PCR amplification of the target DNA. The results indicated that the DNA obtained by the applied protocols was sufficiently pure for further molecular analyses.

5

Protozoan impact on bacterial biofilm formation

72%

Rychert K., Neu T. R.

Biological Letters

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2010

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tom 47

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nr 1

6

Indigenous microflora responses to introduction of cyanogenic strains of Pseudomonas fluorescens into soil

72%

Piotrowska-Seget Z., Kozdroj J.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 1

7

The development of bacterial communities as affected by pH and soil type

72%

Weyman-Kaczmarkowa W., Pedziwilk Z.

Acta Microbiologica Polonica

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1999

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tom 48

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nr 1

8

Molecular analysis of temporal changes of a bacterial community structure in activated sludge using denaturing gradient gel electrophoresis [DGGE] and fluorescent in situ hybridization [FISH]

72%

Ziembinska A., Raszka A., Truu J., Surmacz-Gorska J., Miksch K.

Polish Journal of Microbiology

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2007

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tom 56

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nr 2

Wastewater treatment based on activated sludge is known to be one of the most effective and popular wastewater purification methods. An estimation of microbial community variability in activated sludge allows us to observe the correlation between a particular bacterial group's appearance and the effectiveness of the removal of chemical substances. This research is focused on microbial community temporal changes in membrane bioreactors treating wastes containing a high level of ammonia nitrogen. Samples for this study were collected from two membrane bioreactors with an activated sludge age of 12 and 32 days, respectively. The activated sludge microbial community was adapted for the removal of ammonia nitrogen up to a level of 0.3 g NH₄⁺-N g/VSS/d (VSS - volatile suspended solids). The methods - denaturing gradient gel electrophoresis (DGGE) based on 16S rRNA gene PCR products and fluorescent in situ hybridization (FISH) with 16S rRNA gene probes - revealed significant differences in the microbial community structure in the two bioreactors, caused mainly by a difference in sludge age. According to the results obtained in this study, a bioreactor with a sludge age of 12 days is characterized by a much higher microbial community diversity than a bioreactor with a sludge age of 32 days. Interestingly, the appearance of particular species of nitrifying bacteria was constant throughout the experiment in both bioreactors. Changes occured only in the case of the Nitrosomonas oligotropha lineage bacteria. This study demonstrates that the bacterial community of bioreactors operating with different sludge ages differs in total community structure. Nevertheless, the changeability of the bacterial community structure did not have any influence on the efficiency of nitrification.

9

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Formation of bacterial and fungal communities in the rhizosphere of soybean [Glycine max. [L.] Merrill] and their antagonism towards phytopathogens

72%

Patkowska E.

Journal of Plant Protection Research

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2001

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tom 41

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nr 2

The purpose of the studies conducted in the years 1996-1998 was to determine the quantitative and qualitative composition of bacterial and fungal communities in rhizosphere of soybean cultivated in monoculture and non-rhizosphere soil. Besides, the proportion of bacteria and fungi, which were distinguished by their antagonistic effect towards soil-borne pathogens was established. A microbiological analysis of 1g of dry weight of soil from rhizosphere of soybean resulted in 3.21 x 10⁶ to 8.70 x 10⁶ bacterial colonies and from 70.51 x 10³ to 123.74 x 10³ fungal colonies. In the case of non-rhizosphere soil, 3.50 x 10⁶ to 4.75 x 10⁶ bacterial colonies and 16.16 x 10³ to 51.38 x 10³ fungal colonies were obtained. Besides, soybean cultivation in monoculture had a negative effect on the number of antagonistic isolates of bacteria (Bacillus spp., Pseudomonas spp.) and fungi (Gliocladium spp., Penicillium spp., Trichoderma spp.). Smaller numbers of antagonistic bacteria and fungi in rhizosphere soil of soybean cultivated in monoculture as compared to non-rhizosphere soil, can prove little biological activity, which results in a worse phytosanitaty condition of the soil.

10

Analysis of the bacterial and fungal community profiles in bulk soil and rhizospheres of three mungbean [Vigna radiata (L.) R. Wilczek] genotypes through PCR-DGGE

58%

de los Reyes A. M. M., Ocampo E. T. M., Manuel M. C. C., Mendoza B. C.

International Letters of Natural Sciences

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2020

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tom 77

Each plant species is regarded to substantially influence and thus, select for specific rhizosphere microbial populations. This is considered in the exploitation of soil microbial diversity associated with important crops, which has been of interest in modern agricultural practices for sustainable productivity. This study used PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) in order to obtain an initial assessment of the bacterial and fungal communities associated in bulk soil and rhizospheres of different mungbean genotypes under natural field conditions. Integrated use of multivariate analysis and diversity index showed plant growth stage as the primary driver of community shifts in both microbial groups while rhizosphere effect was found to be less discrete in fungal communities. On the other hand, genotype effect was not discerned but not inferred to be absent due to possible lack of manifestations of differences among genotypes based on tolerance to drought under non-stressed environment, and due to detection limits of DGGE. Sequence analysis of prominent members further revealed that Bacillus and Arthrobacter species were dominant in bacterial communities whereas members of Ascomycota and Basidiomycota were common in fungal communities of mungbean. Overall, fungal communities had higher estimated diversity and composition heterogeneity, and were more dynamic under plant growth influence, rhizosphere effect and natural environmental conditions during mungbean growth in upland field. These primary evaluations are prerequisite to understanding the interactions between plant and rhizosphere microorganisms with the intention of employing their potential use for sustainable crop production.

11

The impact of organic carbon and ammonia load in wastewater on ammonia-oxidizing bacteria community in activated sludge

58%

Cydzik-Kwiatkowska A., Wojnowska-Baryla I.

Polish Journal of Microbiology

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2008

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tom 57

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nr 3

The experiment was carried out in two SBR reactors differing in ammonia load (reactor R1 - ammonia load of 130 mg N-NH₄ x d⁻¹, reactor R2 - ammonia load of 250 mg N-NH₄ x d⁻¹). Feeding conditions in the reactors were switched from favoring autotrophic nitrification through favoring heterotrophic processes and back to autotrophic conditions. Observations of ammonia-oxidizing bacteria (AOB) community changes were based on PCR-RFLP analysis of amplified amoA gene fragments and AOB genetic diversity was evaluated on the base of the number of different amoA gene forms. When only carbonates were introduced with wastewater restriction patterns established about day 23 and 28 at ammonia load of 250 and 130 mg N-NH₄ x d⁻¹, respectively. In both reactors statistically higher number of different amoA gene forms was observed when only carbonates were present in wastewater in comparison to conditions in which sodium acetate was introduced to the reactors. The AOB participation in activated sludge was higher at ammonia load of 250 mg N-NH₄ x d⁻¹ but their genetic diversity was lower in comparison with this observed at ammonia load of 130 mg N-NH₄ x d⁻¹.

Ograniczanie wyników

The biochemical diversity of heterotrophic bacterial cmmunities in high mountain lakes from Sierra Nevada (Granada, Spain)

Distribution of microbial-selected populations in Lake North Mamry by fluorescent in situ hybridization

Molecular analysis of bacterial community diversity in sequencing batch reactor [SBR] operating in autotrophic conditions

Bacterial community DNA extracted from soils polluted with heavy metals

Protozoan impact on bacterial biofilm formation

Indigenous microflora responses to introduction of cyanogenic strains of Pseudomonas fluorescens into soil

The development of bacterial communities as affected by pH and soil type

Molecular analysis of temporal changes of a bacterial community structure in activated sludge using denaturing gradient gel electrophoresis [DGGE] and fluorescent in situ hybridization [FISH]

Formation of bacterial and fungal communities in the rhizosphere of soybean [Glycine max. [L.] Merrill] and their antagonism towards phytopathogens

Analysis of the bacterial and fungal community profiles in bulk soil and rhizospheres of three mungbean [Vigna radiata (L.) R. Wilczek] genotypes through PCR-DGGE

The impact of organic carbon and ammonia load in wastewater on ammonia-oxidizing bacteria community in activated sludge