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1

Autolysis of Listeria monocytogenes strains isolated from food and clinical specimens

100%
Cibik R., Cetinkaya F., Gunes N., Ozakin C., Soyutemiz G. E.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 11
1242-1244
Autolysis of Listeria monocytogenes strains isolated from clinical and food specimens were tested under starvation conditions. Late exponential phase harvested bacterial cells were transferred in potassium phosphate buffer (100 mM, pH 7) and incubated at 37°C to induce lysis. At the end of the 48 h incubation period a heterogeneous autolysis profile was observed among the tested strains. All the clinical strains exhibited high autolysis i.e. above 75%. Meanwhile, the extent of food isolates was not as high as the clinical ones but demonstrated a great variability - ranging between 22 to 88%. An increase in the amount of intracellular markers upon lysis was also measured in the higher autolytic clinical strain - UVF172, compared to low autolytic food strain - UVF114. The results obtained from the study provided evidence that autolysis in L. monocytogenes is strain dependent and revealed that tested clinical isolates had a higher level of autolysis than food isolates.
2

Brewers’ yeast autolysate - new, functional food component

100%
Swiderski F., Podpora B., Szterk A., Waszkiewicz-Robak B.
Polish Journal of Food and Nutrition Sciences
|
2011
|
tom 61
|
nr Suppl.1
3
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Shelf life extension of shrimp (white) using modified atmosphere packaging

100%
Kalleda R. K., Han I. Y., Toler J. E., Chen F., Kim H. J., Dawson P. L.
Polish Journal of Food and Nutrition Sciences
|
2013
|
tom 63
|
nr 2
Wild caught shrimp can have a shortened shelf life compared to farm raised shrimp due to handling and on-ship limitations. The loss of freshness in shrimp is partly due to autolytic reactions caused by endogenous enzymes such as polyphenol oxidase. The objective of this study was to determine the effect of sulfi tes combined with Modified atmosphere packaging (MAP) on the shelf life of non-frozen shrimp. Fresh South Atlantic white shrimp were subjected to one of four treatments, no bisulfite rinse-air packaged, 1.25% bisulfite rinse-air packaged, 1.25% bisulfite rinse-MAP (60% CO2, 18% O2, 22% N2) and 1.25% bisulfite rinse-MAP (36% CO2, 64%N2). The quality and freshness of shrimp was measured by determining total aerobic bacterial populations, package gas headspace analysis, shrimp volatiles (GC-MS), meat pH, nucleotide degradation, and visual analysis. Fresh non-frozen shrimp treated with a combination of sulfites and MAP maintained the shelf life of fresh shrimp up to 10 days while shrimp in non-MAP without sulfite and non-MAP with sulfite developed black spots within 2 and 6 days, respectively. Both MAP treatments slowed bacterial growth while the MAP with high CO2 and with O2 was more effective in preventing off odors and nucleotide degeneration.
4

Susceptibility of Listeria monocytogenes strains isolated from dairy products and frozen vegetables to antibiotics inhibiting murein synthesis and to disinfectants

100%
Popowska M., Olszak M., Markiewicz Z.
Polish Journal of Microbiology
|
2006
|
tom 55
|
nr 4
The susceptibility of 96 strains of Listeria monocytogenes isolated from food to antibiotics and disinfectants currently used in human therapy, veterinary, medicine and food industry was determined by a standard operating procedure - broth dilution method. Antimicrobial agents included the β-lactams ampicillin and penicillin, the lantibiotic nisin, and the disinfectants benzalkonium chloride and chlorhexidine gluconate. Among the studied strains we found 13 strains with 8-fold, 7 strains with 16-fold and 2 strains with 32-fold decreased susceptibility to ampicillin, as determined by MIC, compared to wild type reference strain. Interestingly, the mentioned strains were isolated from frozen vegetables and soups, none of the isolates from dairy products showed any elevated resistance to the studied antimicrobial agents. The occurrence in food products of strains with increased resistance to ampicillin is disquieting, especially since β-lactams are the most frequent antibiotic of choice in the therapy of infections caused by the pathogen.
5

Autolysis of Listeria monocytogenes

80%
Popowska M., Kloszewska M., Gorecka S., Markiewicz Z.
Acta Microbiologica Polonica
|
1999
|
tom 48
|
nr 2
6

Activation of the baker's yeast autolysis

70%
Stecka K., Komorowska A., Mrowka E., Grzybowski R.
Polish Journal of Food and Nutrition Sciences
|
1999
|
tom 08
|
nr 2
The activation effect of baker's yeast Saccharomyces cerevisiae autolysis was examined under standard conditions with addition of certain compounds: organic acids, vitamins, sugars, inorganic salts, ethanol, and their mixtures. The efficiency of autolysis in the presence of mixed activators (glucose/ethanol/sodium chloride in the concentration of 0.5/1.0/1% by wt.), increased by 50%, or by 80% when 0.15% chitosan solution was applied. These figures are related to the amount of protein expelled in spontaneous autolysis. The highest increase of protein liberated from yeast cells, above 100%, was obtained when the process was activated with organic acids at the concentration of 0.5%.
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