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A total of 439 measurements of downward ultraviolet irradiance in North European coastal waters have been analysed,half of which have been taken from other authors. The depths Z(10%) where the irradiance is reduced to 10% of its surface value vary by one order of magnitude in the open coastal waters,b oth at wavelengths of 310 nm (0.3–10.4 m) and 380 nm (1.2–13.0 m). In the fjords and estuaries the depth ranges are reduced to 0.08–6.1 m at 310 nm and 0.18–7.7 m at 380 nm. Mixing with saline ocean waters can increase these light penetration depths to more than 10 m,while river water can reduce them to a few centimetres.
A coastal aquifer has a dynamic seaward boundary at the beach face where physical and ecological processes are influenced by oceanic water level fluctuations. Many basic groundwater concepts and the role of the impact of groundwater seepage on beach ecosystems are still poorly understood. Studies are needed to improve our understanding of the relationships between surface and subsurface flow processes on beaches. This is particularly helpful in clarifying the interaction of the physical processes, biodiversity and productivity of sandy beaches, sediment transport and coastal structure stability and modern beach nourishment techniques. As the estimation of infiltration into beach sand is very difficult to carry out under real sea conditions, a control led large-scale laboratory experiment was carried out in the LargeWave Channel in Hannover (Germany) as part of a project supported by the European Community (contract HPRI-CT-2001-00157). First part of the paper describes the technology applied in the experiment and reports some preliminary results.
Enoyl coenzyme A hydratase short chain 1 (ECHS1) is an important part of the mitochondrial fatty acid β-oxidation pathway. Altered ECHS1 expression has been implicated in cancer cell proliferation. This study assessed ECHS1 expression in human gastric cancer cell lines and investigated the effects of ECHS1 knockdown on gastric cancer cell proliferation and migration. The human gastric cancer cell lines SGC-7901, BGC-823 and MKN-28, and the immortalized human gastric epithelial mucosa GES-1 cell line were analyzed for ECHS1 protein levels using western blot. The effectiveness of ECHS1-RNA interference was also determined using western blot. Proliferation and migration of the siECHS1 cells were respectively measured with the CCK-8 and transwell assays. Phosphorylation of PKB and GSK3β was assessed using western blot. ECHS1 protein levels were significantly higher in poorly differentiated cells than in well-differentiated cells and immortalized gastric epithelial mucosa cells. Stable expression of ECHS1 shRNA was associated with an over 41% reduction in the ECHS1 protein levels of siECHS1 cells. Constitutive knockdown of the ECHS1 gene in siECHS1 cells was associated with significantly inhibited cell proliferation and migration. We also observed decreased levels of PKB and GSK3β phosphorylation in siECHS1 cells. ECHS1 expression is increased in human gastric cancer cells. Increased ECHS1 expression activates PKB and GSK3β by inducing the phosphorylation of the two kinases. ECHS1 may play important roles in gastric cancer cell proliferation and migration through PKB- and GSK3β-related signaling pathways.
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