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The age-dependent induction of apoptosis-inducing factor [AIF] in the human semitendinosus skeletal muscle

100%

Park S. Y., Kim H. Y., Lee J. H., Yoon K. H., Chang M. S., Park S. K.

Cellular and Molecular Biology Letters

2010

tom 15

nr 1

1-12

To assess the dependence on age of the expression of apoptosis regulatory proteins in the human semitendinosus muscle, we measured the expression levels of several apoptosis-related genes, including apoptosis-inducing factor (AIF), Bax, Bcl-2, caspase-3 and heat shock protein 70 (HSP70), using RT-PCR, immunohistochemistry and TUNEL assays. We found that the DNA fragmentation was proportional to the age of the tissues sample donors. The expression levels of AIF were significantly elevated (by 10 to 25%) in semitendinosus tissue samples from older individuals, but the Bax, Bcl-2, caspase-3 and HSP 70 levels remained almost constant. This data suggests that the morphological and functional changes observed in aged human semitendinosus muscle correlates with the apoptosis of muscle cells through the induction of AIF.

Non-Abeta component of Alzheimer's disease amyloid and amyloid beta peptides evoked poly(ADP-ribose) polymerase-dependent release of apoptosis-inducing factor from rat brain mitochondria

100%

Adamczyk A., Czapski G. A., Jesko H., Strosznajder R. P.

Journal of Physiology and Pharmacology. Supplement

2005

tom 56

nr 2

5-13

Amyloid beta peptide (Aß) and non-Aß component of Alzheimer’s disease amyloid (NAC) are involved in pathomechanism of Alzheimer's Disease (AD) and are deposited in the AD brain in the form of senile plaques. However, the mechanism of their neurotoxicity is not fully understood. In this study the sequence of events involved in NAC and Aß peptides evoked toxicity was investigated in brain slices, synaptosomes and in subcellular fractions. Radio-, immunochemical, spectrophotometrical methods and DNA electrophoresis were used in this study. Our data indicated that Aß 1-40 (25 µM) and NAC (10 µM) peptides induced liberation of free radicals and massive DNA damage that lead to activation of DNA bound enzyme poly(ADP-ribose) polymerase-1 (PARP-1). In consequence of these processes apoptosis-inducing factor (AIF) was released from mitochondria and was translocated to nucleus. The inhibitor of PARP, 3-aminobenzamide significantly decreased AIF release from mitochondria and its translocation. Both peptides under the investigated conditions had no effect on caspase-3 activity. Our data indicated that Aß and NAC peptides stimulate AIF-dependent apoptotic pathway that seems to be caspase independent process. The inhibition of PARP-1 may protect the brain against Aß and NAC toxicity.

Current views on apoptosis in theory and medical practice

80%

Szpringer E., Lutnicki K.

Polish Journal of Veterinary Sciences

2003

tom 06

nr 1

Apoptosis is a kind of cell death essential for normal functioning and survival of most multicellular organisms. Apoptosis plays an important role in physiological processes and in pathophysiology of some chronic diseases, including autoimmunity, cancer, lymphoma, AIDS, neurodegeneration and others. The present paper describes the interdisciplinary pathogenesis of programmed cell death, the mechanisms inducing apoptosis and the role of apoptosis in some physiological phenomena and in medical practice.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Journal of Physiology and Pharmacology. Supplement

1 Polish Journal of Veterinary Sciences

1 Adamczyk A.

1 Chang M. S.

1 Czapski G. A.

1 Jesko H.

1 Kim H. Y.

1 Lee J. H.

1 Lutnicki K.

1 Park S. K.

1 Park S. Y.

1 Strosznajder R. P.

1 Szpringer E.

1 Yoon K. H.

1 2010

1 2005

1 2003

Wyniki wyszukiwania

The age-dependent induction of apoptosis-inducing factor [AIF] in the human semitendinosus skeletal muscle

Non-Abeta component of Alzheimer's disease amyloid and amyloid beta peptides evoked poly(ADP-ribose) polymerase-dependent release of apoptosis-inducing factor from rat brain mitochondria

Current views on apoptosis in theory and medical practice