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The aim of this study was to evaluate the effects of dietary supplementation of bamboo leaf extract (BLE) on the growth performance, antioxidant traits, immune function, and lipid metabolism of weaning piglets. After weaning at 21 days, two hundred and forty healthy piglets (Large White × Landrance) were randomly assigned to 4 treatments with 6 pens and 10 piglets per pen. The control group (Ctr) received a maize-soyabean based diet, and the test groups received the control diet plus 0.5%, 1%, or 2% (w/w) BLE, respectively. The experiment was carried out for 5 weeks. At the end of it, average daily feed intake in the 1% BLE group was decreased (p < 0.05). Plasma concentrations of malondialdehyde were decreased with supplementation of 1% and 2% BLE. Immunoglobulin G concentrations and lysozyme activity in plasma were significantly increased in piglets supplemented with BLE. Diets with 1% and 2% BLE increased (p < 0.05) plasma concentrations of low density lipoprotein-cholesterol and reduced (P < 0.01) high density lipoprotein-cholesterol levels. Higher triglyceride concentrations were observed in the 0.5% (P < 0.01) and 2% (p < 0.05) BLE groups. In conclusion, these novel findings demonstrate that supplementation of BLE to the diet improved the antioxidant activity, immune function, and lipid metabolism of weaning piglets.
To investigate the biological activities of anthocyanins, which are induced by cadmium in A. imbricata, the antioxidant properties of anthocyanins were investigated using various antioxidant assays, namely 1,1 -diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azinobis-(3-ethylbenzthiazoline-6- sulfonic acid) (ABTS) radical scavenging activity, reducing power, and β-carotene bleaching assay. Results showed that anthocyanins exhibited excellent antioxidant activities in all assays and the EC₅₀ values of DPPH radicals scavenging, ABTS radicals scavenging, reducing power and β-carotene bleaching assay were 19.08, 10.69, 40.93, and 44.19 µg∙mL⁻¹, respectively. The Cd²⁺ chelation potency of anthocyanins was also investigated in vitro. Under given conditions, Cd²⁺ chelating ability of anthocyanins increased significantly with increase in contact time, anthocyanins concentration in dialysis tubing and Cd²⁺ concentration in solution. Based on these results, anthocyanins inducibly synthesized by Cd2 treatment was a powerful antioxidant, as well as Cd²⁺ chelator, might play a role in detoxification of Cd in A. imbricata.
Severe burn injury is associated with damage of tissues and organs distant to the area of injury. Although different agents are suggested to play an important role in pathogenesis of the burn disease, disturbed balance between the development of reactive oxygen forms and activity of antioxidants can play a pivotal role. Therefore, the aim of our study was to examine the intensity of lipid peroxidation process in plasma and lung tissues as well as the antioxidant ability of rats subjected to severe burn injury during 48 hrs after the injury. Our results show that severe burn injury causes a significant increase in the level of lipid peroxides in plasma and lung tissues, with a concomitant increase in superoxide dismutase (Cu-Zn SOD) activity in erythrocytes during 48 hrs of the postburn period. Glutathione peroxidase (Se-GPx) activity in whole blood was significantly higher during the first postburn day and then decreased becoming lower than that found in the healthy subjects. Total Antioxidant Status (TAS) and the level of uric acid in plasma also increased. Thus, we conclude that severe burn injury causes the imbalance between the intensity of the lipid peroxidation process and the antioxidant ability of the organism and this can play an important role in the pathophysiology of the burn disease.
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