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1

Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from 2000 to 2002 in Poland

100%
Paciorek J.
Polish Journal of Microbiology
|
2004
|
tom 53
|
nr 4
Minimal inhibitory concentration (MIC) of ampicillin, penicillin, gentamicin, erythromycin, clarithromycin, sulfisoxazole and trimethoprim for 73 Listeria monocytogenes isolates from clinical material samples, food and environment was determined using broth microdilution method. With exception of the sulfonamide all antibacterial agents were active against all tested strains. Resistance to sulfisoxazole (MIC≥512 μg/ml) was detected in case of 30.1% of isolates including 5 of 14 human strains.
2

Antimicrobial susceptibility patterns of Enterobacteriaceae isolated from domesticated animals and the environment in Lake Victoria, Kenya

75%
Wawire S. A., Miruka D. O., Nelson N., Ofulla A.
International Journal of Ecohydrology and Hydrobiology
|
2013
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tom 13
|
nr 4
3
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Phytochemistry and antimicrobial property of fruits of Chrysophyllum albidum against selected clinical isolates

75%
Adeleye S. A., Orji C. C., Braide W., Akaluka C. K.
International Letters of Natural Sciences
|
2016
|
tom 55
The antimicrobial activity of methanolic and aqueous extracts of Chrysophyllum albidum fruits was investigated against clinical isolates(Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and Candida albicans). Qualitative phytochemistry of the plant indicated that the plant contained Flavonoids, Steroids, Alkaloids, Tannin, Anthraquinone and Cardiac glycosides while Saponins were reported absent. The maximum activity of the aqueous extracts in the test isolates was observed on Staphylococcus aureus, which showed clear zones with diameters of 24.0mm, 20.0mm and 16.5mm at concentrations of 100mg/ml, 50mg/ml and 25mg/ml respectively while it had low activity on Klebsiella pneumonia, with clear zones of inhibition of 15.0mm, 12.0mm and 10.5mm at same concentrations. On the other hand, Methanolic extracts activity on Staphylococcus aureus produced clear zones of 21.0mm, 17.5mm and 12.0mm at concentrations of 200mg/ml, 100mg/ml and 50mg/ml respectively as its best activity while the it had least observed activity on Klebsiella pneumonia with clear zones of 14.0mm, 11.5mm and 10.5mm at same concentrations. The aqueous extracts had greater activity than the methanolic extracts at same concentrations. Therefore, the fruit of the plant can be a good source of remedy in phytomedicine.
4

Identification and antimicrobial susceptibility of microbial agents of otitis externa in dogs

63%
Kahya Demirbilek S., Yilmaz O.
Medycyna Weterynaryjna
|
2019
|
tom 75
|
nr 02
In this study, a total of 277 unmedicated dogs with otitis externa were used. Overall, 413 agents were isolated from 277 ear swab samples; 52.7% of the cases were mono-infections (146 cases), and 37.1% of the cases were poly-infections (103 cases). In 10.1% (28) of the cases, neither bacteria nor yeasts were isolated. Coagulase-positive Staphylococcus spp. were the most frequently isolated bacteria and were found in 90 (21.8%) of the samples. Fifty-eight samples, (14%) were positive for Staphylococcus aureus, 51 (12.3%) for Pseudomonas aeruginosa, 27 (6.5%) for Proteus mirabilis, 27 (6.5%) for Malassezia pachydermatis, 21 (5%) for Corynebacterium spp., 21 (5%) for β-haemolytic Streptococcus spp., 15 (3.6%) for Staphylococcus pseudointermedius, 12 (2.9%) for Proteus spp., 12 (2.9%) for Escherichia coli, 9 (2.1%) for Acinetobacter calcoaceticus, 7 (1.6%) for Trichophyton mentagrophytes, 5 (1.2%) for Staphylococcus auricularis, and 46 (11.1%) for different bacteria and yeasts. A total of 14 different bacteria and yeasts were isolated and identified. Kirby-Bauer antibiotic susceptibility testing was carried out for 10 different antibiotics. The bacterial isolates were found to be resistant to amoxicillin-clavulanic acid (45%), gentamycin (28%), ampicillin/cloxacillin (69%), tobramycin (28%), amikacin (23%), enrofloxacin (47%), chloramphenicol (58%), doxycycline (65%), lincomycin/spectinomycin (58%) and polymyxin B (62%). In conclusion, it is important to test the antimicrobial sensitivity of aetiological agents of otitis externa before treatment so as to prevent the development of antibiotic resistance in bacteria and yeasts.
5

Antimicrobial susceptibility of metallo-beta-lactamase positive and negative Klebsiella pneumoniae strains isolated from intensive care unit patients

63%
Sekowska A., Hryniewicz W., Gniadkowski M., Deptula A., Kusza K., Gospodarek E.
Polish Journal of Microbiology
|
2010
|
tom 59
|
nr 1
67-69
Carbapenem resistance in Gram-negative bacteria is a worldwide increasing and one of the most disturbing problems, given these antibiotics are drugs of choice in the treatment of infections caused by extended-spectrum-beta-lactamase producing strains. In this study the antibiotic susceptibility of metallo-beta-lactamase-positive and negative Klebsiella pneumoniae strains isolated from intensive care unit (ICU) patients was evaluated. The presence of genes encoding MBLs was determined with a commercial kit hyplex® MBL ID (BAG HEALTH CARE). The MBL-producing isolates were the first K.pneumoniae isolates of this kind identified in Poland. It seems that methods for detecting MBLs in Enterobacteriaceae should be included in contemporary standards of microbiological diagnostics in the country.
6

The antimicrobial susceptibility of Helicobacter pylori strains isolated from children and adults with primary infection in the Lower Silesia region, Poland

63%
Gosciniak G., Biernat M., Grabinska J., Binkowska A., Poniewierka E., Iwanczak B.
Polish Journal of Microbiology
|
2014
|
tom 63
|
nr 1
The resistance of microorganisms to antibiotics has become a serious issue in recent years in the therapy of bacterial infections. This problem also concerns the treatment of infections caused by Helicobacter pylori strains. The aim of this study was to evaluate the frequency of primary resistance of H. pylori strains isolated from children and adults. The subject of the research was 105 strains of H. pylori isolated from children and 60 strains from adults in the Lower Silesia Region in the years 2008–2011. Antimicrobial susceptibility to the following antibiotics was assessed: amoxicillin (AC), clarithromycin (CH), metronidazole (MZ), tetracycline (TC), levofloxacin (LEV) and rifabutin (RB). Among the strains isolated from children, 33.3% were resistant to CH, 44.8% to MZ whereas 1.9% of strains were resistant simultaneously to CH, MZ and LEV. Among 60 strains isolated from adults, 23.3% were resistant to CH, 66.7% to MZ, and 6.7% to LEV. Moreover, 16 multidrug resistant strains were isolated from adults, including 12 resistant to CH and MZ, 3 to MZ and LEV, and 1 to CH, MZ and LEV. All examined strains were susceptible to AC, TC and RB. The high incidence of resistance to CH and MZ suggests that standard triple therapies may not be useful as first-line treatment in Poland without earlier susceptibility testing.
7

Antibiotic resistance patterns and occurrence of mecA gene in Staphylococcus intermedius strains of canine origin

63%
Kizerwetter-Swida M., Chrobak D., Rzewuska M., Binek M.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 1
We have evaluated 102 Staphylococcus intermedius isolates of canine origin for susceptibility to antimicrobial primary agents, i.e. penicillin, amoxicillin, amoxicillin with clavulanic acid, cefuroxime, trimethoprim/sulfonamides, neomycin, streptomycin, gentamicin, norfloxacin, tetracycline, vancomycin, erythromycin and secondary agents, i.e. chloramphenicol, ciprofloxacin, lincomycin, teicoplanin, rifampicin, imipenem, mupirocin. Antimicrobial sensitivity was examined using the disk diffusion method and performed according to NCCLS quidelines. Methicillin resistance was detected using the disk diffusion method with oxacillin, and the occurrence of mecA gene was detected by PCR. Resistance to streptomycin, penicillin, amoxicillin, neomycin, followed by tetracycline was predominant. From 14 mecA-positive strains, 12 were multidrug-resitant, and the remaining two showed atypical susceptibility. One strain resistant to oxacillin in the disc diffusion method was mecA-negative, suggesting a different mechanism of resistance. Our results indicate that the emergence of S. intermedius resistance to methicillin may be underestimated. In case of clinical multidrug-resitant S. intermedius isolates, resistance to methicillin should be considered.
8

Antimicrobial susceptibility of staphylococci isolated from affected with mastitis cows

63%
Malinowski E., Klossowska A., Kaczmarowski M., Lassa H., Kuzma K.
Bulletin of the Veterinary Institute in Puławy
|
2002
|
tom 46
|
nr 2
9

Survey of antimicrobial susceptibility among some pathogenic and commensal bacteria isolated from pigs in Lithuania

51%
Ruzauskas M., Klimiene I., Zienius D.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 04
397-400
Antimicrobial susceptibility of isolated enterotoxigenic E. coli, Salmonella enterica serovar Choleraesuis, Pasteurella multocida and Streptococcus suis from pigs was tested in the Veterinary Institute of the Lithuanian Veterinary Academy. Commensal E. coli and Enterococcus faecalis were also included in the testing as commensal bacteria. Clinical and pathological material was investigated from various regions of the country. Isolation and identification of bacteria was done using common methods. The agar diffusion method according to NCCLS guidelines was applied for antimicrobial susceptibility testing. Enterotoxigenic E. coli showed the highest resistance to tetracycline (67%), ampicillin (52%) and sulfamethoxazole-trimethoprim (43%). Not less than 90% of these bacteria were susceptible to colistin, florfenicol and ceftiofur. Salmonella Choleraesuis demonstrated the highest resistance to tetracycline (53%). Florfenicol, ceftiofur and enrofloxacin were effective against most strains of salmonella. Pasteurella multocida in most cases were susceptible to all the tested antimicrobials, however 20% of the isolates were resistant to sulfamethoxazole-trimethoprim. Streptococcus suis demonstrated the highest resistance to tetracycline (43%), lincomycin (40%), sulfamethoxazole- -trimethoprim (40%), and erythromycin (30%). Ceftiofur was the most effective against S. suis. Commensal E. coli showed less resistance than enterotoxigenic E. coli, however not less than 25% of isolates were resistant to tetracycline, sulfamethoxazole-trimethoprim and ampicillin. All the tested Enterococcus faecalis were susceptible to vancomycin and ceftiofur and 80% of enterococci were resistant to tetracycline.
10

Occurrence of the aacA4 gene among multidrug resistant strains of Pseudomonas aeruginosa isolated from bronchial secretions obtained from the Intensive Therapy Unit at University Hospital in Bialystok, Poland

51%
Sacha P., Jaworowska J., Ojdana D., Wieczorek P., Czaban S., Tryniszewska E.
Folia Histochemica et Cytobiologica
|
2012
|
tom 50
|
nr 2
11

Prevalence and drug resistance of Salmonella in dogs and cats in Xuzhou, China

51%
Wei L., Yang C., Shao W., Sun T., Wang J., Zhou Z., Chen C., Zhu A., Pan Z.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 2
12

Primary and secondary bacteremia caused by Proteus spp.: epidemiology, strains susceptibility and biofilm formation

51%
Kwiecinska-Pirog J., Skowron K., Gospodarek-Komkowska E.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 4
Proteus spp. is an etiological factor of urinary tract and bloodstream infections. The aim of this study was the retrospective analysis of susceptibility of Proteus spp. strains isolated from bloodstream infections (BSIs) as well as similarity evaluation of the strains isolated from different clinical samples. Proteus spp. strains were isolated in 2009–2017 from hospital patients. Identification was based on the colony’s morphology and biochemical or MALDI-TOF MS analyzes. The antibiotic susceptibility test was done using the diffusion method. Biofilm formation was evaluated with microplate method using TTC. Bacteremia caused by Proteus spp. was found in 97 patients, mainly secondary to urinary tract infection. Most of the strains were susceptible to piperacillin with tazobactam (95.9%) and amikacin (86.7%). Elderly patients have a higher risk of mortality after BSIs caused by Proteus spp. A detailed analysis was made for randomly chosen 26 strains isolated from 11 patients with Proteus mirabilis bacteremia. Using PFGE, we found that 10 (90.9%) isolates, collected from different clinical specimens of the same patient, were genetically identical.
13

First isolation of Clostridium difficile PCR-ribotype 027-toxinotype III in Poland

51%
Pituch H., Bakker D., Kuijper E., Obuch-Woszczatynski P., Wultanska D., Nurzynska G., Bielec A., Bar-Andziak E., Luczak M.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 3
Of 175 Clostridium difficile strains isolated from patient hospitalized in one academic hospital in Warsaw between 2005-2006 one isolate belonged to PCR-ribotype 027/toxinotype III. This isolate had tcdA, tcdB, binary toxin genes (cdtA and cdtB), a 18-bp deletion and a 1 bp deletion at 117 position in the tcdC gene. Antimicrobial susceptibility tests revealed high level resistance to erythromycin, moxifloxacin and gatifloxacin. This is a first report of the 027 strain of C. difficile in Poland.
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