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New trends in mink vaccinology

100%

Uttenthal A., Christensen J., Hansen M., Dalsgaard K., Casal I., Meloen R., Langeveld J., Henriksen P., Ostergaard J., Alexandersen S.

Zeszyty Naukowe. Przegląd Hodowlany

1996

tom 28

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Reactivity of heat-stable Leptospira antigenic preparation used in enzyme-linked immunosorbent assay for detection of antibodies in swine serum

67%

Wasinski B., Pejsak Z.

Polish Journal of Veterinary Sciences

2012

tom 15

nr 1

Serology plays an important role in laboratory diagnosis of leptospirosis. Apart from the most often used microscopic agglutination test (MAT), enzyme-linked immunosorbent assay (ELISA) seems to be useful especially in screenings of animal herds. The ELISA used for detection of antibodies against selected Leptospira serogroups in swine serum samples was investigated during the study. An essential element of this test is heat-stable antigenic preparation from cultures of Leptospira interrogans serovars Icterohaemorrhagiae, Pomona and L. borgpetersenii serovar Sejroe. The aim of the present study was to identify and analyze ELISA heat-stable antigen fractions playing a role in the reaction with leptospiral antibodies indicated in swine serum. Reactivity of the three-component antigenic preparation was compared in immunoblotting with reactivity of six heat-stable antigenic preparations made from the following single serovars: L. interrogans serovars Icterohaemorrhagiae, Pomona, Canicola, L. borgpetersenii serovars Sejroe, Tarassovi and L. kirshneri serovar Grippotyphosa. All antigenic preparations were submitted to SDS-PAGE and transferred to a nitrocellulose membrane using a semidry system. After the transfer, the membrane was incubated with diluted swine serum containing antibodies specific for one of the six above mentioned Leptospira serovars. For the three-component antigenic preparation and antigens prepared from single serovars the immunoblot revealed reaction of sera with fractions of the 20-26 kDa region and around the 14.5 kDa region. The investigated heat-stable Leptospira antigenic preparation contains fractions demonstrating serogroup- and species-specificity. Fraction 20-26 kDa showed serogroup-specific activity, whereas the fraction around 14.5 kDa showed species-specific activity.

Ograniczanie wyników

1 Polish Journal of Veterinary Sciences

1 Zeszyty Naukowe. Przegląd Hodowlany

1 Alexandersen S.

1 Casal I.

1 Christensen J.

1 Dalsgaard K.

1 Hansen M.

1 Henriksen P.

1 Langeveld J.

1 Meloen R.

1 Ostergaard J.

1 Pejsak Z.

1 Uttenthal A.

1 Wasinski B.

1 2012

1 1996

Wyniki wyszukiwania

New trends in mink vaccinology

Reactivity of heat-stable Leptospira antigenic preparation used in enzyme-linked immunosorbent assay for detection of antibodies in swine serum