Symptoms of shoot dieback and leaf yellowing followed by leaf chlorosis were observed in naturally infected roses 'Frisco' and 'Suela', cultivated in a commercial greenhouse in Poland. The presence of phytoplasma was demonstrated in affected plants by nested polymerase chain reaction (PCR) with R16Fl/RO and Pl/p7 primer pairs in the first round followed by a second one with RI6F2n/R2, fNrA, Pc399/p1694, R16(1)Fl/Rl and PllfArev primer pairs. Restriction fragment length polymorphism (RFLP) analysis of PCR products (primed with primers R16F2n/R2) was done using enzymes Alul, Msel, Rsal and HpaII. Restriction profiles obtained with these enzymes were identical to those of reference strain AYl belonging to aster yellows phytoplasma group, subgroup l-B (16SrI-B). Nested PCR products from roses 'Frisco' and 'Suela' were sequenced. Analysis of sequences confirmed that the phytoplasma infecting those roses could be classified to aster yellows phytoplasma group, subgroup B.
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