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1

Production and characterization of amphiploids of Aegilops kotschyi and Ae. biuncialis with Secale cereale, and of backcross hybrids of Ae. biuncialis x S. cereale amphiploids with 2x and 4x S. cereale

100%
Wojciechowska B., Pudelska H.
Journal of Applied Genetics
|
2005
|
tom 46
|
nr 2
Amphiploids (2n = 6x = 42) of Ae. kotschyi and Ae. biuncialis with self-compatible S. cereale were produced from F₁ sterile hybrids (2n = 3x = 21) through colchicine treatment and callus tissue regeneration. The amphiploids resembled the F₁ plants in overall morphology, but were larger in all respects and self-fertile. The spikelets consisted mostly of 3 well-developed florets. Selfed seeds were obtained from some colchicine-doubled sectors and callus regenerates. Most of the produced seeds were well developed. Backcrosses between amphiploids and rye (2x and 4x) resulted in obtaining (Ae. biuncialis x S. cereale amphiploid) x S. cereale hybrids via embryo culture. The BC₁ plants (2n = 4x = 28 and 2n = 5x = 35, respectively) were phenotypically intermediate between the parents and vigorous in vegetative growth. Some seeds were obtained only from the 35-chromosome BC₁ hybrids.
2

Isozymes in Aegilops kotschyi and Ae.biuncialis x Secale cereale hybrids and Ae.kostchyi x S.cereale amphiploids in relation to their parents

100%
Kalinowski A., Wojciechowska B., Borzyszkowska E.
Journal of Applied Genetics
|
2003
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tom 44
|
nr 1
3

rRNA gene expression and location in triticale assayed by silver staining and in situ hybridisation techniques

100%
Lima-Brito J., Carvalho A., Matos C., Heslop-Harrison P., Guedes-Pinto H.
Plant Breeding and Seed Science
|
2005
|
tom 51
4

Pollen morphology and two-dimensional patterns of pollen coat and protoplast proteins in Aegilops kotschyi x Secale cereale amphiploids

100%
Kalinowski A., Klimko M., Wojciechowska B.
Acta Biologica Cracoviensia. Series Botanica
|
2005
|
tom 47
|
nr 2
Amphiploid pollen of Aegilops kotschyi x Secale cereale was compared to the parental form by SEM, and comparatively measured using light microscopy. Pollen grains of amphiploids were larger and more variable in total and pore diameter than the parents. Amphiploid pollen was prolate, subprolate and prolate-spheroidal in shape. The exine of Ae. kotschyi AK-2 and AK-3 had a delicate verrucate surface, whereas Secale cereale S14 exine had a verrucate surface. Amphiploid pollen grain surfaces were more or less similar to those of the parents: delicate verrucate, verrucate and well verrucate. The sculpture of parental and amphiploid pollen grains showed conspicuous granulation. All amphiploids produced pollen with one pore with an operculum, surrounded by a well-defined annulus. Sporophytically produced peptides from the pollen coat and gametophytically produced peptides from the protoplast were analyzed separately by two-dimensional gel electrophoresis. Two accessions ofAe. kotschyi (AK-2, AK-3) showed differences in the 2-D patterns of peptides from both the pollen coat and the protoplast. The majority of pollen coat and protoplast peptides of the parents were detected in the amphiploids, but a number of parental peptides were absent. All the amphiploids possessed peptides in their pollen coat and protoplast not detected in the corresponding pollen fractions of the parents. No relation between colchicine and in vitro amphiploid production and its 2-D patterns was observed. The results of pollen morphology and pollen protein analysis are convergent.
5
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Epidermal features of glumes and florets in Aegilops geniculata Roth and Aegilops peregrina (Hack.) Maire et Weiller x Secale cereale L. hybrids, amphiploids and parental forms

80%
Klimko M., Wysakowska I.
Steciana
|
2015
|
tom 19
|
nr 1
Micromorphological features of glumes, lemmas and paleas were examined under light and scanning electron microscopes in amphiploids of Aegilops geniculata, Ae. peregrina × Secale cereale and their parental species. The principal features include the size and shape of silica bodies, crown cells, prickles, macrohairs, stomata and the morphology of long cells. The SEM observations of glumes separate two parental forms of Aegilops by the type of prickles and types of wax on palea surface. The abaxial surfaces of lemmas in the parental species and amphiploids show the most taxonomical features. Amphiploids in many features exhibit an extended range of variation in comparison to their parental species, particularly in stomatal length. Epidermal features in amphiploids are inherited after Ae. geniculata and Ae. peregrina. Results of this study provided data on new quantitative and qualitative traits of the glumes, lemmas and paleas in the studied taxa.
6

Two-dimensional leaf isozyme patterns of Aegilops kotschyi x Secale cereale amphiploids

80%
Kalinowski A., Wojciechowska B.
Acta Physiologiae Plantarum
|
2004
|
tom 26
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nr 1
7

Aegilops-rye amphiploids and substitution rye used for introgression of genetic material into rye (Secale cereale L.)

71%
Apolinarska B., Wisniewska H., Wojciechowska B.
Journal of Applied Genetics
|
2010
|
tom 51
|
nr 4
The valuable genes of Aegilops biuncialis, Ae. ovala, Ae. kotschyi, and Ae. variabilis were transferred to rye, by crossing Aegilops-rye amphiploids with tetraploid and diploid substitution rye. The C-banded karyotype of the BC₁ and BC₂ generations of amphiploids with 4x substitution rye and BC₁ with 2x substitution rye showed great variation in chromosome number and composition. In the BC₁ generation of amphiploids with 4x and 2x substitution rye, seed set success rate and germination rate varied depending on origin. However, plant sterility in all cross combinations of amphiploids with 4x and 2x substitution rye resulted in their elimination from further experiments in the BC₃ and BC₂ generations, respectively. In backcrosses of 4x substitution rye with amphiploids Ae. variabilis x rye 4x, fertile 4x rye plants containing Aegilops chromatin were produced in the BC₂ generation.
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