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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

100%

Woloszyn A., Kotlowski R.

Roczniki Państwowego Zakładu Higieny

2017

tom 68

nr 3

Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.

alpha-Amanitin induced apoptosis in primary cultured dog hepatocytes

67%

Magdalan J., Ostrowska A., Piotrowska A., Izykowska I., Nowak M., Gomulkiewicz A., Podhorska-Okolow M., Szelag A., Dziegiel P.

Folia Histochemica et Cytobiologica

2010

tom 48

nr 1

58-62

Ograniczanie wyników

1 Folia Histochemica et Cytobiologica

1 Roczniki Państwowego Zakładu Higieny

1 Dziegiel P.

1 Gomulkiewicz A.

1 Izykowska I.

1 Kotlowski R.

1 Magdalan J.

1 Nowak M.

1 Ostrowska A.

1 Piotrowska A.

1 Podhorska-Okolow M.

1 Szelag A.

1 Woloszyn A.

1 2017

1 2010

Wyniki wyszukiwania

A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

alpha-Amanitin induced apoptosis in primary cultured dog hepatocytes