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Herba Polonica
|
2009
|
tom 55
|
nr 2
59-68
In present study the essential oils composition and antioxidant activities of the essential oils as well as methanolic extracts of leaves, fruits and roots of Biebersteinia multifida were evaluated. The GC-MS analysis of the essential oils resulted in the identification of 36 compounds. Thymol (16.5–38.4%), β-caryophyllene (9.8–15.5%), 1,8-cineol (5.8–18.4%), α-pinene (0.9–14.3%) and β-pinene (2.3–12.4%) were the main components. The samples were subjected to screening for their possible antioxidant activity by using DPPH and β-carotene-linoleic acid assay. In the first case, the radical scavenging activity of the essential oil and methanolic extract of fruits were superior to all other essential oils and extracts (IC50=16.7). In the case of the linoleic acid system, oxidation of the linoleic acid was effectively inhibited by essential oils and extracts of different parts. The fruit extracts showed 95.4±2.15% inhibition, which is comparible to synthetic antioxidant BHT, curcumine and ascorbic acid.
Methanol and ethanol extract showed antibacterial activity in range of 0-11 at 10 mg/mL at against all tested bacterial species. Methanol extract with amoxicillin exhibited effective inhibition E. coli. This combination showed antagonistic effect against B. subtilis and S. aureus. Interaction of ethanol extract with amoxicillin also showed effective inhibition against E. coli and decreased activity against B. subtilis and S. aureus. Methanol extract did not show inhibition against P. aeruginosa but it enhanced activity of ciprofloxacin on combination. Ethanol extract also exhibited effective inhibition against P. aeruginosa on combination with ciprofloxacin. But antagonist effect was observed on interaction of both extract with ciprofloxacin against other bacterial species. Interaction of methanol and ceftazidime showed mostly decreased or indifferent effect against all bacterial species. Ethanol extract with ceftazidime resulted in higher inhibition against E. coli. Combination of methanol extract with erythromycin showed higher inhibition against E. coli and also very effective inhibition against S. aureus. This combination resulted in decreased activity against B. subtilis. Ethanol extract with erythromycin also showed synergistic effect against S. aureus and effective inhibition against E. coli. These results indicate that both methanol and ethanol extract has potential to enhance the combination effect with erythromycin against S. aureus and E. coli.
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