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Sunflower downy mildew caused by Plasmopara halstedii is one of the most potentially important diseases. So far, a complete, major gene resistance (Pl) has been used successfully, but with the appearance of eight races in France since 2000, research on more durable resistance was undertaken. In this study, we present new results concerning the evolution of pathogenicity of P. halstedii under conditions of re-enforced infection and different Pl gene selection pressures. Moreover, we imagine the evolution of virulence and aggressiveness of P. halstedii under a mixture model of sunflower inbred lines carrying the two types of resistance which may enhance durable resistance against it. Examples of host-parasite interactions including the influence of plant mixture models against pathogens are equally presented to understand how the pathogen develops its pathogenicicity.
Sunflower downy mildew caused by Plasmopara halstedii is one of the most potentially important diseases. So far, a complete, major gene resistance (Pl) has been used successfully. But, with the appearance of eight races in France since 2000, research on more durable resistance was undertaken. In this study, we presented new results concerning the evolution of pathogenicity in P. halstedii under conditions of re-enforced infection and different Pl gene selection pressure. Moreover, we studied the evolution of virulence and aggressiveness of P. halstedii under a mixture model of sunflower inbred lines carrying the two types of resistance (qualitative and quantitative). This sunflower model may enhance durable resistance against P. halstedii.
A set of 14 aggressive Phythophthora infestans (Mont.) de Bary isolates with a wide range of virulence, was stored for eight years (2002-2010) in liquid nitrogen at -196°C (209 samples) and under paraffin oil at 7°C (70 test tubes). The survival rate of samples stored in liquid nitrogen was scored as 88%, 45 days after thawing. The revived isolates were passaged through potato tissues four times and tested for virulence on a set of 11 Black’s differentials. The mean number of virulence factors per isolate, of these P. infestans isolates evaluated before storage, was 7.4. The isolates stored in liquid nitrogen and under paraffin oil and passaged four times through potato tissues showed a similar mean number of virulence factors per isolate, 7.3 and 6.9, respectively. Isolates stored under paraffin oil showed no expression of factor avr5, but expression of this factor occurred in six isolates after liquid nitrogen treatment. The initial expression of this factor occurred in four isolates. Before storage, the average aggressiveness of the tested isolates was assessed as 1.7, on a 1-9 scale, where 1 means the most aggressive. After storage and after four subsequent passages through potato tissues, the mean aggressiveness of isolates stored in liquid nitrogen and under paraffin oil reached the level of 1.5 and 2.1, respectively. The mean aggressiveness was not significantly different from the level of initial assessment.
Reactions of a Neomys fodiens (Pennant, 1771) parous female [FP] to 4 conspecific and 3 N. anomalus Cabrera, 1907 intruders were studied quantitatively in enclosure (135 x 135 cm) experiments. Total time of observations was 30 hr. FP initiated 5.6 intraspecific conflicts per 1 hr (1.95/hr of these were pouncings and fightings) with adult males, 0.8 conflicts/hr with a juvenile female, and 4.7 conflicts/hr with a N. ano­malus adult male. Reactions of N. fodiens parous female were several times more aggressive than interactions between non-breeding 'residents' and 'immigrants' of both water shrew species tested under the same conditions in earlier studies.
The appearance of cavum septi pellucidi (CSP) has been already connected with developmental disorders or with serious psychiatric diseases (schizophrenia, maniac-depressive disorder; aggressiveness or similar). During our investigation of CSP, group of alcoholics seemed to be interesting for investigation and comparison with the groups of schizophrenics and people with violent behavior: We obtained CSP in 205 out of 670 fixed brains (429 male and 241 female) of persons aged from l4 to 89 (mean ~ SD: 53.28 ~16.57), 85 cava belonged to our group of interest. Prevalence of CSP in aforementioned groups was significantly higher than in the rest of examined population. Only in the group of 20 to 39 years of age all kinds of obtained pathologies were present. Alcoholics with CSP suffered from cardiovascular diseases and died accidentally and aggressive persons were the only drug addicts in our examined group and they had the shortest life span. 34 out of 4l schizophrenics had no data about prior diseases and disorders and they were the ones who committed suicide frequently. Intensive demyelinization of corpus callosum and transformation of potential space between laminae of septum pellucidum to the actual one could explain the CSP appearance among alcoholics. To our opinion, cavum septi pellucidi might be used as an additional marker of organic brain changes in long term alcohol abuse, as a consequence of demyelinization due to lipolytic effect of ethanol, and lower cerebral blood flow.
The aim of this work was 1) to investigate the genetic diversity of H. annosum s. str. populations, 2) to recognize genets ability of their aggressiveness to Scots pine. The genetic similarity among genets varied from 0% to 74%. Totally different genets were found in 17.9% of cases. The lowest similarity occurred among 20.7% of genets and it was calculated on 18%. Six months after inoculations of Scots pine stems by H. annosum s. str. the distance of wood colonization by mycelium was measured. Wood was colonized to a distance varying from 0.52 cm to 2.06 cm. The most aggressive genets were isolated from stumps, and the less aggressive from trees' stems. The infections in stands caused by H. annosum s. str. basidiospores and appearance of a new pathogen's organisms, which might distinguish by high aggressiveness, could increase a damages in these stands. There is still a need to use a biological control against root rot disease to reduce spreading of a new generations of pathogens.
Formica sanguined Latr. occasionally takes wood ants as its slaves. Eleven nests of the former were experimentally provided with various amounts (10,000-250,000) of pupae of F. polyctena Foerst. or F. rufa L., and thus, mixed colonies with different proportions of slaves (29-96%) were obtained. The slaves destroyed the F. sanguinea colonies by eliminating their brood andqueen(s) (with no aggressiveness towards adult workers). The following year, even though F. sanguinea workers were still present, the slaves adopted young queens of their own species. Before their complete emancipation, the slaves fought on the side of the slavers during conflicts with conspecific ants, even against their former nestmates. Emancipation restored mutual comity between the ex-slaves and the unenslaved F. polyctena. At the same time, the ex-slavers lost their aggressiveness towards alien F. polyctena (but not vice versa). The mixed colonies were raided by foreign F. sanguinea. The studies were carried out in the Gorce Mts (the Western Carpathians) from 1987 to 1991.
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