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1

Real-time PCR and agar plating method to predict Fusarium verticillioides and fumonisin B1 content in Nigerian maize

100%
Adejumo T. O., Hettwer U., Nutz S., Karlovsky P.
Journal of Plant Protection Research
|
2009
|
tom 49
|
nr 4
399-404
Eighty maize grain samples collected in Nigeria were investigated for fumonisin B1 (FB1) content and Fusarium verticillioides colonization. F. verticillioides DNA was quantified by species-specific real-time PCR and living propagules of the fungus were counted by agar-plating method. FB1 was detected in 55 (68.7%) of the total samples (mean: 98.5 μg/kg, range: 10 to 714 μg/kg) at 10 μg/kg detection limit. The mean amount of F. verticillioides DNA determined by real-time PCR was 49.7 μg/kg (range: 10-126.7 μg/kg), while agar plate method showed the presence of F. verticillioides in 45 samples (mean incidence: 21.0%, range: 6.7-60.0%). There was correlation ties between F. verticillioides DNA by real time PCR and fungal colonization by agar plate method (R = 0.71, p = 00001 at 95% confidence level), and means of FB1 and F. verticillioides DNA in the yellow and white maize were significantly different. Despite the high consumption of maize in Nigeria, the amount of FB1 ingested by consumers appears to be low. The estimated daily intake of fumonisins was 0.21 μg/kg body weight per day.
2

Strongyloides stercoralis in patients on corticosteroids therapy using enzyme-linked immunosorbent assay and gelatin particles indirect agglutination tests: a diagnostic approach

100%
Ahmed S. A., El-Moselhy A., El-Moammaly A., El-Shewy K.
Acta Parasitologica
|
2019
|
tom 64
|
nr 2
3

Occurrence of seed-borne Fusarium on triticale seed in relation to seed decay and seedling root rot

84%
Grzelak K.
Hodowla Roślin, Aklimatyzacja i Nasiennictwo
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1993
|
tom 37
|
nr 4
4

Current state of world standardization in the toxicometric methods for testing of wood preservatives

67%
Wazny J.
Drewno. Prace Naukowe. Doniesienia. Komunikaty
|
2007
|
tom 50
|
nr 178
125-136
5

Screening for soil streptomycetes from North Jordan that can produce herbicidal compounds

67%
Bataineh S. M. B., Saadoun I., Hameed K. M., Arabneh Q.
Polish Journal of Microbiology
|
2008
|
tom 57
|
nr 4
A total of 231 different soil Streptomyces isolates were recovered from 16 different locations in North Jordan. They were assessed for their phytotoxic activity on seeds of cucumber (Cucumis sativus L.) and ryegrass (Lolium perenne L.) placed adjacent to a 2 cm wide Streptomyces culture strips grown at 28C° for 3 weeks on starch casein nitrate (SCN) agar. Phytotoxicity was ascertained on the basis of suppressed seed germination, discoloration of the root tip, reduced root and the shoot growth and eventual death of the root. Twenty one of the isolates exhibited adverse effect against growth of germinated cucumber seeds, germination and growth of ryegrass seeds. Using filter paper bioassay method, culture filtrate from the SCN broth of the isolate R9; identified as Streptomyces aburaviensis, significantly inhibited seed germination, radicle and shoot growth of ryegrass, reduced radicle and shoot growth of cucumber and suppressed the shoot growth of milk thistle (Silybum marianum L.). Also, culture filtrate from the glucose-peptone-molasses (GPM) broth diluted (1:1) with sterilized distilled water caused complete inhibition of seed germination of redroot pigweed (Amaranthus retroflexus L.). Dichloromethane extracted fraction of S. aburaviensis (strain R9) culture filtrate from GPM broth completely inhibited seed germination of ryegrass when applied at doses of 3 and 5 mg of dry weight, and the seedling growth of cucumber and milk thistle was severely reduced by the same doses.
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