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The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium [HCV29] cell line

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Litynska A., Pochec E., Hoja-Lukowicz D., Kremser E., Laidler P., Amoresano A., Monti C.

Acta Biochimica Polonica

2002

tom 49

nr 2

There is a growing line of evidence that glycosylation of a and β subunits is important for the function of integrins. Integrin a3β1, from human ureter epithelium cell — line HCV29, was isolated by affinity chromatography on laminin GD6 peptide. Characterization of its carbohydrate moieties was carried out using sodium dodecyl sul- fate/polyacrylamide gel electrophoresis followed by Western blotting on Immobilon P and on-blot deglycosylation with peptide .-glycosidase-F. Profiles of N-glycans for each subunit were obtained by matrix-assisted laser desorption/ionization mass spec- trometry. Our findings demonstrated, in both subunits of integrin a3β1, the presence of complex type oligosaccharides with a wide heterogeneity. Bi- tri- and tetra- antennary structures were the most common, while high-mannose type structures were minor. Also the presence of short poly-. -acetyllactosamine entities was shown. These results show that while the predominant oligosaccharides of both subunits are identical, some slight differences between them do exist.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Amoresano A.

1 Hoja-Lukowicz D.

1 Kremser E.

1 Laidler P.

1 Litynska A.

1 Monti C.

1 Pochec E.

1 2002

Wyniki wyszukiwania

The structure of the oligosaccharides of alpha3beta1 integrin from human ureter epithelium [HCV29] cell line