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1

Active immunization against enzootic pneumonia in pig farm with poor housing and environmental conditions

100%
Siugzdaite J., Garlaite K., Siugzda R.
Medycyna Weterynaryjna
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2005
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tom 61
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nr 01
The objective of the present study was to determine the influence of inactivate vaccine against Mycoplasma hyopneumoniae (M. hyopneumoniae) in a pig farm with poor housing and environmental conditions. The vaccine efficacy investigation was carried out at a medium-sized pig farm. A total of 740 piglets were selected for the experiment. The piglets were divided into 2 equal groups: vaccinated and non-vaccinated. Forty-four piglets were allocated in two groups of twenty-two piglets each. The first group of twenty-two piglets was vaccinated and 14 days later it was revaccinated. The control group of twenty-two non-vaccinated piglets was injected by a placebo. Blood samples with respect to antibody formation against M .hyopneumoniae were taken at 7, 21, 35, 49, 63, 91, 119 and 147 days-of-age and investigated by ELISA. Piglet's life weights were measured individually at the age of 7, 51, 81 and 171 days during their transfer to another unit. Back fat thickness, muscle thickness and lean meat content were measured before slaughter. Pulmonary lesions caused by M. hyopneumoniae were scored at slaughter by the Goodwin method. Lungs with gross lesions were investigated microbiologically. The ELISA tests of serum showed that antibodies against M. hyopneumoniae in the vaccinated group formed after 14 days of the second vaccination and remained till the 147'h day. [n the non-vaccinated group seroconversion started on the 91't day. Dai|y weight gain (DWG) showed an increase of 33 grams (g) in vaccinated pigs compared with non-vaccinated ones. The percentage of lean meat was significantly, greater (difference 3.26%) in the vaccinated group. Greater proportion of lung surface with pneumonic lesions was detected in non-vaccinated pigs using all methods. Eight lung samples with lesions typical of M. hyopneumoniae from the non-vaccinated group were investigated microbiological|y and M. hyopneumoniae was cultured from all specimens (100%). The study demonstrates that pigs vaccinated against M. hyopneumoniae with inactivate bacteria under poor housing and environmental conditions achieved higher DWG, lean meat percentage and lower lung lesions than the pigs from the control group.
2

The influence of active immunisation against GnRH on adrenergic innervation of the porcine testis, epididymis and vas deferens. A quantitative and morphological study

100%
Sienkiewicz W., Kaleczyc J., Wasowicz K., Czaja K., Pidsudko Z., Kotwica J., Lakomy M.
Polish Journal of Veterinary Sciences
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2000
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tom 03
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nr 2
This study investigated the influence of active immunisation against Gn-RH on adrenergic innervation of the porcine testis, epididymis and vas deferens. Seven groups of the boars were used including three control groups (n=9) Gl, G2 and G3 consisting of non-immunised animals aged 10,18 and 26 weeks, respectively, and four groups of immunised pigs (7-9 animals in each group). All the experimental boars (G4-G7) were immunised at the age of 10 weeks. Then, the animals from the two groups (G6, G7) were immunised again at age of 18 weeks. The vasa deferentia from the immunised pigs were collected at the age of 18 weeks (G4, immunised once) and at the age of 26 weeks (G5, immunised once, and two groups, G 6 and G7, immunised twice). Immunisation was accomplished by vaccination with GnRH using vaccine obtained from Peptech Animal Health, Australia. Tissue concentrations of noradrenaline (NA) were determined using high performance liquid chromatography (HPLC), and double-immunolabellings for PGP 9.5, SNAP-25 and DßH were also performed. Immunohistochemistry revealed numerous PGP- or SNAP-25-positive (+) nerve fibres supplying the organs. The vast majority of them contained also DßH. The study revealed a gradual age-dependent decrease in NA tissue concentration and a density of PGP 9.5+, SNAP-25+ and DßH+ innervation of the testis and epididymis in the control animals (G1-G3). However, in the effectively immunised animals (especially in the boars of G7 immunised twice), both NA tissue concentration and the density of the innervation were significantly higher than those found in the animals of the corresponding control groups. With regard to the vas deferens, no distinct differences in both NA tissue concentration and in a density of the innervation between the particular animal groups including those consisting of the control and immunised boars were observed. The results obtained strongly confirm previous suggestions on the existence of an inverse relationship between the nervous and hormonal systems at the level of the porcine testis.
3

Innervation of the prostate gland in the pig after hormonal castration

72%
Czaja K., Sienkiewicz W., Kaleczyc J., Wasowicz K., Majewski M., Lakomy M.
Polish Journal of Veterinary Sciences
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2000
|
tom 03
|
nr 3
This study investigated the influence of active immunization against GnRH on the innervation of the prostate gland in pigs by means of immunohistochemical and quantitative methods. Three control groups of the animals including: Gl- 10 week-old, G2- 18 week-old, G3- 26 week-old and four groups of immunized pigs: G4, G5, G6 and G7 (from 7 to 9 animals in each group) were used. Immunization was accomplished by vaccination against GnRH with a vaccine purchased from the Peptech Animal Health, Australia. The pigs of G7 were immunized with the same peptide but suspended in a Freund's adjuvant. The presence of dopamine ß-hydroxylase (DßH), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and protein gene product 9.5 (PGP) within nerve fibres supplying the prostate gland in the intact animals and in boars undergoing the active immunization was studied using single-or double-labelling immunohistochemistry. Moreover, noradrenaline (NA), NPY and VIP concentrations were estimated in the body of the prostate gland with HPLC and RIA methods. Immunohistochemical studies showed that a density of DßH im- munoreactive (-IR) nerve fibres supplying the prostate in the pigs of G7 was significantly higher than that in the remaining groups. The density of PGP- and NPY-IR nerve fibres was higher after immunization in both immature (G4) and mature (G5, 6, 7) pigs than that in the control animals while the density of VIP-IR nerve fibres was very similar. The quantitative studies revealed that NA tissue concentration in the animals of G7 was significantly higher than that in the remaining groups while NPY tissue content was significantly higher in both immature (G4) and mature (G5, 6, 7) animals. The tissue concentration of VIP in all the immunized groups was similar to that observed in the control pigs. The present results show that the most effective immunization was achieved in the animals injected twice according to modified method with Freund's adjuvant (G7) and suggest that in the future, it could be considered as the alternative method of castration.
4

Szczepienie ochronne u ludzi

58%
Nowak T.
Wszechświat
|
1957
|
nr 06
5

Czynniki decydujące o skuteczności szczepień

44%
Pejsak Z.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2017
|
tom 12
|
nr 2
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