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1

Nowe gatunki rodzaju Yersinia i ich znaczenie dla czlowieka oraz zwierzat. I. Y.intermedia, Y. frederiksenii, Y. kristensenii, Y. ruckeri

100%
Staroniewicz Z.
Medycyna Weterynaryjna
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1995
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tom 51
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nr 08
446-448
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effects of oral vaccination against Yersinia ruckeri on oxidative stress biomarkers in gills, liver and heart of rainbow trout (Oncorhynchus mykiss Walbaum)

100%
Tkachenko H., Grudniewska J., Pekala A.
Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
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2017
|
tom 21
The aim of this study was to assess the effect of oral vaccination against Yersinia ruckeri based on oxidative stress biomarkers in different tissues of rainbow trout (Oncorhynchus mykiss Walbaum). Vaccine consisted of three Y. ruckeri strains (O1 serotype) that originated from rainbow trout cultured on the different farms, where fish exhibited clinical signs of enteric redmouth disease. Concentrated vaccine was incorporated in the fish food; treatment was delivers three times at one day intervals. One month after immunization, gills, hepatic and cardiac tissues were sampled. The vaccinated trout showed tissue-specific oxidative stress responses in the gills, liver, and heart. The gill tissue was the most sensitive to oxidative damage among the samples. Accumulation of oxidative stress biomarkers in the rainbow trout was tissue-specific with following accumulation: gills > heart > liver. These results suggest that the trout expressed tissue-specific oxidative stress mechanisms due to anti- Yersinia vaccine treatment. There were no statistically significant alterations in the activities of antioxidant defenses instead superoxide dismutase activity in the hepatic and cardiac tissue and glutathione peroxidase activity in the gills of vaccinated trout. Correlative analysis confirmed the role of catalase in the antioxidant defense in vaccinated trout. The oxidative stress biomarkers, i.e. content of oxidative modified proteins in the gills, and liver, and 2-thiobarbituric acid reactive substances level in the gills, and total antioxidant capacity in the liver, were sensitive to vaccination of trout against Y. ruckeri and may potentially be used as biomarkers in evaluating vaccine toxicity in rainbow trout.
3

Application of anti-stress products in aquaculture: influence of propiscin on the effectiveness of an anti-Yersinia ruckeri vaccine in rainbow trout Oncorhynchus mykiss [Wall.]

100%
Siwicki A. K., Morand M., Kazun K., Keck N., Glabski E., Malaczewska J.
Archives of Polish Fisheries
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2002
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tom 10
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nr 2
Inducing protection against bacterial diseases in fish culture through the application of specific vaccines is one of the most important prevention methods. However, some immunization techniques actually used under hatchery conditions are not as effective as they should be. Therefore, current research is focusing on how to improve the potency and efficacy of the antigens and how to optimally activate the immune response. In our preliminary study, we determined the influence of the anaesthetic Propiscin (IFI, Poland) on the effectiveness of a vaccine applied by intraperitoneal injection or immersion to rainbow trout (Oncorhynchus mykiss). The fish were vaccinated via injection or immersion with an anti-Yersinia ruckeri vaccine (Sanofi, France) under Propiscin-induced anaesthesia or without it. On day 21 after vaccination, all the fish groups were challenged with live Y. ruckeri bacteria (0.2 ml of bacteria at 1 x 10⁶ ml⁻¹ ). Control groups (only anaesthetized and injected PBS or immersed) were infected but not immunized by vaccine. The blood and pronephros were separated before vaccination (10 fish), 21 days after vaccination (10 fish) and 21 days after the challenge (10 fish). ELISPOT assays for the quantification of total immunoglobulin secreting cell (ISC) and specific antibody secreting cell levels (ASC) were used. The total Ig levels in the serum were also measured by spectrophotometric assay and the titers of anti-Y. ruckeri antibody were measured by the micro-agglutination method. Mortalities were collected and tabulated and the presence of pathogen was confirmed by isolation from fish kidneys. The results of this study showed that Propiscin applied at vaccination time increased the nonspecific and specific immune response and protective effectiveness of vaccine after the challenge with pathogenic bacteria. The highest level of total ISC and specific ASC in fish vaccinated by immersion and injections under anaesthesia were observed. The highest total Ig levels and titers of specific antibody in fish vaccinated under anaesthesia were also observed. Cumulative mortality rates were lowest in the fish vaccinated by injection (5%) and immersion (10%) under anaesthesia as compared with fish vaccinated by injection (20%) and immersion (35%) without anaesthesia. Cumulative mortality in the control, non-vaccinated group of fish was 80%.
4

Serological variation among Polish isolates of Yersinia ruckeri

80%
Pekala A., Kozinska A., Antychowicz J.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
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nr 3
One hundred and twenty-six isolates of Yersinia ruckeri originating from different species of fish were collected: 122 from rainbow trout (Oncorhynchus mykiss; Walbaum), three from pike (Esox lucius L.), and one from carp (Cyprinus carpio L.). The O- serotyping of the isolates were carried out for the first time in Poland by microplate agglutination assays according to the Davies procedure. Three O-serotypes were determined: O1, O5, and O7. Serotypes O2 and O6 have not been recognised. Almost all isolates were represented by serotype O1, which originated only from rainbow trout showing classical clinical signs of enteric redmouth disease. The strains representing serotype O5 were only collected from pike and serotype O7 from carp and rainbow trout showing no clinical signs of the diseases.
5

Jersinioza ryb lososiowatych - charakterystyka czynnika etiologicznego i metody jego identyfikacji

80%
Pekala A., Antychowicz J.
Medycyna Weterynaryjna
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2010
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tom 66
|
nr 05
299-301
Yersinia ruckeri, a Gram-negative rod belonging to Enterobacteriaceae family, genus Yersinia, is a causative agent of yersiniosis of salmonids. The size of the active growth cells are 0.75 × 1.0 µm - 3 µm. The presence of flagella are connected with motile ability but non motile bacterium are isolated more frequently. The biochemical characteristics of Y. ruckeri strains are rather homogenous. Y. ruckeri rods in in vitro examination are characterized as quite sensitive to medicines. All of the Polish isolates were sensitive to oxytetracycline, flumeqine and enrofloxacin. Classical bacteriological methods are mainly used for the diagnostics of yersinisis. Tryptone soya agar or 5% blood agar are carried out for the isolation of the microorganism. Selective and facultative mediums such as Furones medium, ROD or Waltman-Shotts media are also used. Identification of the isolated strains is performed by the characteristics of their biochemical properties, after which an analysis of the obtained profiles is done. Commercial API 20E kits are used in the examination of biochemical characteristic of the isolates but it is necessary for the results of the identification to be always supplemented by complementary tests. The plate agglutination test and PCR are carried out as a confirmation of identification of Y. ruckeri rods. This last method, on account of its high sensitivity, is useful in the detection of asymptomatic carriers of bacterium Y. ruckeri.
6

Charakterystyka szczepów chorobotwórczych Yersinia ruckeri i ich znaczenie w immunoprofilaktyce pstrąga tęczowego

80%
Pajdak J., Terech-Majewska E., Platt-Samoraj A., Schulz P., Siwicki A. K., Szweda W.
Medycyna Weterynaryjna
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2017
|
tom 73
|
nr 09
Due to the wide range of distribution, tendency to stationary persistence and high susceptibility of salmonid fish, vaccination against yersiniosis is an important part of prophylactic programs in many trout farms. The use of autovaccines prepared on the basis of isolates from a given fish farm is considered to be more effective than the use of commercial preparations. The aim of the study was to analyze cases of the disease in 2015 and to try to explain the failure of implemented prophylactic program based on autovaccination. Y. ruckeri strains isolated from one fish farm, from disease outbreaks in 2006, 2008 and 2015, as well as strains used for vaccine production were analyzed. Biotype evaluation, biochemical properties using API 20E assays, serotyping, and molecular identification were performed. All analyzed strains belonged to biotype 2. Two different profiles were obtained in API 20E tests. The first was obtained for vaccine strains and from disease cases in 2006 and 2008. The second was obtained for the strain isolated from the disease case in 2015. None of the profiles have been interpreted as Y. ruckeri in the APIweb. The identification of bacteria was confirmed by PCR. Disease strains and two vaccine strains belonged to the O1 serotype. Two of the vaccine strains have been qualified as O5 serotype. The obtained results indicate that the disease outbreak in 2015 was due to the emergence of a strain with increased pathogenicity and the use of an autovaccine based on strains belonging to the O5 serotype, considered to be less pathogenic. Immunization with the use of the O5 serotype does not provide complete protection against infection with other serotypes, so that fish remained susceptible to O1 serotype, which caused the disease in 2015. The results suggest the need for extending the diagnostics of strains used for the production of autovaccines.
7

Wystepowanie Yersinia ruckeri u ryb lososiowatych w Polsce i fenotypowa charakterystyka izolatow

80%
Kozinska A., Pekala A.
Medycyna Weterynaryjna
|
2004
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tom 60
|
nr 08
880-882
8

Jersinioza ryb lososiowatych - epizootiologia choroby, metody jej zwalczania

80%
Pekala A., Antychowicz J.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 06
374-377
Yersiniosis has been spreading around the world, and the classical symptoms of the disease are associated with serotype O1 of this bacterium. Y. ruckeri has been isolated from different fish species but the most susceptible to infection are salmonids, especially rainbow trout. Infected fish and asymptomatic carriers are the main source of the infection, spreading bacteria with feces. The pathogenesis mechanisms of yersiniosis are not well known as yet. Gills are regarded as the entry route of Y. ruckeri rods but the likelihood of the disease depends on the virulence of the given strain. Characteristic clinical signs of yersiniosis, such as haemorrhages around the oral cavity, are causes by extracellular products (ECPs) of Y. ruckeri. Haemorrhages are found in internal organs as well. Post mortem examination showed a dysfunction of the swim bladder which consisted in the presence of bloody effusion fluid in its cavity. Reduction of stress factors during the culture and transport of fish as well as the application of vaccines as an immunoprophylactic effectively prevent yersiniosis. A number of medicines have been applied in the therapy of yersiniosis. Sulphonamides and quinolones are among the most frequently used.
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Muscle biochemistry in rainbow trout Oncorhynchus mykiss following Yersinia ruckeri vaccination

80%
Tkachenko H., Grudniewska J., Pekala A.
Baltic Coastal Zone. Journal of Ecology and Protection of the Coastline
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2016
|
tom 20
Vaccination of rainbow trout against Yersiniosis confers a high degree of protection to the fish (Raida et al. 2011). On the other hand, vaccination could alter metabolitic reactions in organisms. Therefore, exploring the effects of vaccination against Y. ruckeri on health condition of trout in general, and oxidative stress biomarkers and biochemical alterations in different tissues specifically, would be valuable. This prompted us to investigate theeffects of vaccination against Y. ruckeri on muscle function, and the oxidative mechanism underlying those effects, by detecting relevant lipid peroxidation (2-thiobarbituric acid reactive substances, TBARS) and protein oxidation biomarkers (aldehydic derivatives and ketonic derivatives) as well as biochemical alterations (aminotransferases and lactate dehydrogenase activity, lactate and pyruvate levels) in rainbow trout Oncorhynchus mykiss following Y. ruckeri vaccination at first month after oral immunization. Concentrated vaccine with inactivated by formalin Y. ruckeri strains was enclosed by fish feed, and was administered three times every other day. Rainbow trout from each group were euthanized 30 days after the immunization, and then muscle tissue were sampled for analysis. The TBARS level in the muscle tissue of vaccinated group was at same level compared to unhandled group. The ketonic derivatives of oxidatively modified proteins in the trout following Y. ruckeri vaccination at first month after immunization were significantly increased compared to the level in the controls, while the aldehydic derivatives of oxidatively modified proteins were non-significantly increased. Pyruvate level was increased by 47% (p = 0.013) in vaccinated trout compared to values of untreated fish. Lactate level, aminotransferases and lactate dehydrogenase activities were nonsignificantly altered in vaccinated trout. Our results suggest that vaccination could promote the activation of the gluconeogenic substrate-providing enzymes, as well as substrates for aerobic metabolism that might in turn contribute to increase of oxidatively modified proteins. The oxidative stress biomarkers, i.e. content of oxidative protein damage, as well as biochemical enzymes and substrates were sensitive to vaccination of trout against Y. ruckeri and may potentially be used as biomarkers in evaluating vaccine toxicity in rainbow trout. From a practical point of view, the results may be useful in relation to studies of infections and the development, administration and uptake of new vaccines applicable for large amounts of fish.
10

Wrażliwość in vitro na chemioterapeutyki izolatów Yersinia ruckeri pochodzących od ryb z ośrodków hodowlanych na terenie Pomorza Środkowego w latach 2013-2016

71%
Lewandowska A., Bogurska A., Nyk J., Chorazy R.
Komunikaty Rybackie
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2018
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nr 2
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