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Belonging to Class II of transposable elements, En/Spm transposons are widespread in a variety of distantly related plant species. Here, we report on the sequence conservation of the transposase region from sequence analyses of En/Spm-like transposons from Poaceae species, namely Zingeria biebersteiniana, Zingeria trichopoda, Triticum monococcum, Triticum urartu, Hordeum spontaneum, and Aegilops speltoides. The transposase region of En/Spm-like transposons was cloned, sequenced, and compared with equivalent regions of Oryza and Arabidopsis from the gene bank database. Southern blot analysis indicated that the En/Spm transposon was present in low (Hordeum spontaneum, Triticum monococcum, Triticum urartu) through medium (Zingeria bieberstiana, Zingeria trichopoda) to relatively high (Aegilops speltoides) copy numbers in Poaceae species. A cytogenetic analysis of the chromosomal distribution of En/Spm transposons revealed the concurence of the chromosomal localization of the En/Spm clusters with mobile clusters of rDNA. An analysis of En/Spm-like transposase amino acid sequences was carried out to investigate sequence divergence between 5 genera — Triticum, Aegilops, Zingeria, Oryza and Arabidopsis. A distance matrix was generated; apparently, En/Spm-like transposase sequences shared the highest sequence homology intra-generically and, as expected, these sequences were significantly diverged from those of O. sativa and A. thaliana. A sequence comparison of En/Spm-like transposase coding regions defined that the intra-genomic complex of En/Spm-like transposons could be viewed as relatively independent, vertically transmitted, and permanently active systems inside higher plant genomes. The sequence data from this article was deposited in the EMBL/GenBank Data Libraries under the accession nos. AY707995-AY707996-AY707997-AY707998-AY707999-AY708000-AY708001-AY708002-AY708003-AY708004-AY708005-AY708005-AY265312.
In animal feeding soluble dietary fibres have been considered as the main antinutritive factor. High level of soluble pentosan content (SPC) in grains of triticale, which is used greatly as a feeding cereal, decreases feed intake in chickens and piglets, hampers digestion and makes droppings very sticky resulting in inhibition of growth. Variability of SPC was assessed spectrophotometrically within the recombinants of hexaploid triticale with synthetic amphitetraploid Triticum monococcum/Secale cereale to evaluate the possibilities of selection in respect of low soluble pentosan content. Standard triticale lines LT 176/10 and LT 522/6 used in crosses as a gene recipient parents had 125% and 128% of SPC in wheat check cultivars. Surprisingly high variation in SPC of grain was found within the set of introgressive triticale lines resulting from recombination with amphitetraploids. The coefficient of variation (CV%) amounted to 24.7. Some of the studied introgressive lines of triticale had the grain SPC lower than wheat. Theses results make the breeding of low SPC triticale promising.
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