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Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

100%

Rohilla M. S., Tiwari P. K.

Journal of Applied Genetics

2008

tom 49

nr 2

167-182

DNA-based identification of species for phylogenetic analysis as well as forensic identification is widely being carried out with the help of polymerase chain reaction (PCR). In this study, a successful effort has been made to identify 5 species of Indian freshwater turtles, including 3 hard-shell turtles (Geoemydidae), i.e. Kachuga dhongoka, K. kachuga and Geoclemys hamiltoni, and 2 species of soft-shell turtles (Trionychidae), i.e. Aspideretes gangeticus and Lissemys punctata punctata, by using a well-optimized PCR-RFLP method. The analysis of nucleotide sequence variations in the PCR-amplified mitochondrial cyt-b genes (encoding cytochrome b) from the 5 species revealed its usefulness in the taxonomic differentiation of these species. On the basis of cyt-b sequence data and the PCR-RFLP pattern, a phylogeny was developed to resolve the genetic relationships between these species, living in the same habitat type. In comparison, the PCR-RFLP of mitochondrial 16S rDNA genes appeared less decisive in analysing phylogenetic relationships or even in species differentiation. Further, the molecular method (PCR-RFLP) developed here is simple, rapid, reliable and reproducible; hence it can be routinely applied for species identification, essential for conservation and management of endangered chelonian species.

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A new soft-shelled trionychid turtle of the genus Khunnuchelys from the Upper Cretaceous Bostobe Formation of Kazakhstan

80%

Danilov I. G., Vitek N. S., Averianov A. O., Glinskiy V. N.

Acta Palaeontologica Polonica

2015

tom 60

nr 1

Previously unpublished trionychid turtle material from the Upper Cretaceous (Santonian–lower Campanian) Bostobe Formation from the Baybishe and Baykhozha localities in Kazakhstan is described. The material represents a new species of Khunnuchelys, a large, skull-based clade of Cretaceous Asian trionychids. Concordant with other partial skulls and fragmentary specimens described previously, Khunnuchelys lophorhothon sp. nov. has the unusual features of a beaklike maxilla and a vaulted, expanded triturating surface. In addition, the specimens reveal novel features including a constricted skull roof. Although estimates of the length of the carapace differ depending on estimation method, the skull belonged to a turtle of comparable size to the shell-based species “Trionyx” kansaiensis from the same formation. It is likely that K. lophorhothon and “T.” kansaiensis are synonymous, but this can be proved only by a find of associated skull and shell material.

Ograniczanie wyników

1 Acta Palaeontologica Polonica

1 Journal of Applied Genetics

1 Averianov A. O.

1 Danilov I. G.

1 Glinskiy V. N.

1 Rohilla M. S.

1 Tiwari P. K.

1 Vitek N. S.

1 2015

1 2008

Wyniki wyszukiwania

Restriction fragment length polymorphism of mitochondrial DNA and phylogenetic relationships among five species of Indian freshwater turtles

A new soft-shelled trionychid turtle of the genus Khunnuchelys from the Upper Cretaceous Bostobe Formation of Kazakhstan