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The aim of the work was to examine the health status of 11 highbush blueberry (Vaccinium corymbosum L.) cultivars cropped on the plantations in a south-eastern region of Poland as well as to determine the composition of fungus species colonizing their stems. The performed observations of health status indicated that stems with disease symptoms were found on almost all bushes. Three types of symptoms were found: spots on stems, from which mainly Topospora myrtilli was isolated, widespread stem necrosis inhabited by Phomopsis archeri and necrosis of stem tops caused by Botrytis cinerea. Moreover, the following isolates of fungi were obtained Cytospora, Phoma, Fusarium, Alternaria and Seimatosporium vaccinii, Myxothyrium leptideum and Sordaria fimicola. It was confirmed that the most malignant pathogen of highbush blueberry in the studied region was Topospora myrtilli.
The growth of Topospora myrtilli isolates, obtained in the years 2001-2003, was studied on PDA medium at –10, +2, +6, +12, +18, +22 and +28oC. The growth and sporulation of the isolates were observed at +22oC on the following culture media: PDA, maltose – MA and mineral – MSA, as well as on two versions of oat media, one containing 20 g of oat fl akes per litre (OA-20), and the other containing 50 g of oat fl akes per litre (OA-50). The observations of the linear growth of colonies of the studied strains were carried out during a period of 21 days, whereas the formation of morphological structures until the 52nd day of cultivation. It was found that T. myrtilli can grow and sporulate in a wide range of temperature, even at +2oC. The pathogen growth activates with the increase of temperature, up to the optimal temperature, i.e. from +18oC to +22oC. The increase of temperature to +28oC did not favour the mycelium growth and prevents pathogen sporulation. PDA, OA-20, OA- 50 and MA media can be considered to be the most suitable for the growth and development of T. myrtilli, due to the intensive growth and formation of typical macro- and microscopic features. It is necessary to emphasise the great usefulness of oat medium, especially OA-50, for the cultivation of Topospora myrtilli, in the aspect of sporulation of the fungus. It was found that mineral medium is unsuitable due to the formation of mycelium with an untypical structure and colouration as well as late conidial sporulation.
In presented studies was undertaken the attempt to determine the effectiveness of such preparations as Biochikol 020 PC and Biosept 33 SL in limiting the growth of T myrtilli in vitro. Moreover, the protective effect of these biopreparations and Dithane M45 80WP for highbush blueberry stems was determined. The studies carried out in vitro indicated that Biochikol 020 as well as Biosept 33 SL significantly limited the growth and development of pathogen. Very strong inhibition of hyphae growth and its deep degradation were caused by Biosept 33 SL in concentration 0.3%. Similarly, in experiment on protecting action of selected preparations for stems of highbush blueberry against T. myrtilli, Biochikol 020 and Biosept 33 SL inhibited the growth of pathogen. But its protective effects were weaker in comparison to Dithane M45 80WP because after the application of this preparation the development of disease symptoms caused by T. myrtilli was significantly lower.
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