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Genetic diversity of North African Thymus algeriensis in Tunisia: Population structure and implication for conservation

100%

Ben El Hadj Ali I., Guetat A., Boussaid M.

Dendrobiology

2012

tom 67

The genetic diversity within and among nine natural populations of Thymus hirtus Willd. subsp. algeriensis (Boiss. et Reut.) Murb. from different geographical and bioclimatic zones were assessed using Random Amplified Polymorphic DNA data. A total of 154 bands were generated from seven selected primers. 141 bands were polymorphic (P=91.56%). The genetic diversity within a population based on Shannon's index which was high (H'pop=0.307) and varied according to bioclimatic zones. A high genetic differentiation among populations (GST=0.335 and FST=0.296) was revealed, suggesting a population isolation and a low level of gene flow among them. The major proportion of the variation was attributable to individual differences within populations. The genetic structure is in accordance with geography distances. The Neighbour- joining tree based on Nei's and Li's genetic distance among individuals showed that individuals from each population clustered together. The UPGMA dendrogram based on FST values revealed three clusters each of them includes populations closuring to their geographical origin. The high genetic structure of populations resulted from genetic drift caused both by the habitat fragmentation and the low size of most populations. The in situ conservation should interest all populations. The ex situ conservation should be based on the collection of seeds rather within than among populations because of the maximum of variation was revealed within populations.

Zroznicowanie chemiczne dziko rosnacych populacji Thymus pulegioides L., Thymus alpestris TAUSCH. ex A.KERN. oraz Thymus kotschyanus BOISS. and HOHEN.

80%

Osinska E., Szymczak P.

Zeszyty Problemowe Postępów Nauk Rolniczych

2007

tom 517

nr 2

539-545

The experiment was conducted in 2003-2004. The aim of the research was to investigate the chemical variability of three thyme species (Thymus pulegioides L., Thymus alpestris Tausch., Thymus kotschyanus Boiss. & Hohen) under „in situ” conditions. Six localities were selected and fixed by GPS. Samples of raw materials from the stands were collected, dried and chemically analysed. The content of essential oil and its chemical composition as well the content of flavonoids, tannins, polyphenolic acids and bitters were determined. The obtained results indicated significantly chemical differences between the investigated populations of Thymus species. The content of essential oil in the dry raw material ranged from 0.46 ml·100 g⁻¹ DM to 0.93 ml·100 g⁻¹ DM. The content of tannins was similar for T. pulegioides (5.44% on the average). However the content of tannins for T. alpestris ranged from 2.56% to 7.12%. The highest content of polyphenolic acids was determined in the herb of T. pulegioides (2.61%) from „Jaworzec”.

Ograniczanie wyników

1 Dendrobiology

1 Zeszyty Problemowe Postępów Nauk Rolniczych

1 Ben El Hadj Ali I.

1 Boussaid M.

1 Guetat A.

1 Osinska E.

1 Szymczak P.

1 2012

1 2007

Wyniki wyszukiwania

Genetic diversity of North African Thymus algeriensis in Tunisia: Population structure and implication for conservation

Zroznicowanie chemiczne dziko rosnacych populacji Thymus pulegioides L., Thymus alpestris TAUSCH. ex A.KERN. oraz Thymus kotschyanus BOISS. and HOHEN.